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Serological testing for antibodies is promising for surveillance; determining useful thresholds will require collection of serological data from settings with different prevalence of the indicator trachomatous inflammation\u2014follicular (TF). Dried blood spots were collected during trachoma mapping in two districts each of Togo and Democratic Republic of the Congo. Anti-Ct antibodies were detected by multiplex bead assay (MBA) and three different lateral flow assays (LFA) and seroprevalence and seroconversion rate (SCR) were determined. By most tests, the district with\u2009&gt;\u20095% TF (the elimination threshold) had five\u2013sixfold higher seroprevalence and tenfold higher SCR than districts with\u2009&lt;\u20095% TF. The agreement between LFA and MBA was improved using a black latex developing reagent. 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