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The aim of this study was to evaluate the effectiveness of short-range UV-LED irradiation in postmenopausal osteoporosis using ovariectomized mice. Preliminary experiments identified the time of onset of osteoporosis after ovariectomy (8\u00a0weeks) in our model. We have set up a total of 4 groups (n\u2009=\u20098\/group); vitamin D-repletion with UV irradiation (Vit.D+UV+), vitamin D-repletion without UV irradiation (Vit.D+UV\u2212), vitamin D-deficiency with UV irradiation (Vit.D-UV+), vitamin D-deficiency without UV irradiation (Vit.D-UV\u2212), and. From 8\u00a0weeks after ovariectomy, UV was irradiated for 24\u00a0weeks. At the time of 16 and 24\u00a0weeks\u2019 irradiation, serum Vit.D levels, various markers of bone metabolism, bone mineral density, and bone strength were evaluated, and histological analyses were performed. In addition, muscle strength was analyzed. Serum 25-hydroxyvitamin D [25 (OH) D] levels at 40 and 48\u00a0weeks of age were increased in the Vit.D-UV+\u2009group compared to the Vit.D-UV\u2212group. Cortical thickness evaluated with micro-CT and strength of bone were significantly higher in Vit.D-UV+\u2009group than those in Vit.D-UV\u2212\u2009group. There was no difference in muscle strength between Vit.D-UV+\u2009group and Vit.D-UV\u2212\u2009group. No obvious adverse effects were observed in UV-irradiated mice including skin findings. Short-range UV irradiation may ameliorate postmenopausal osteoporosis associated with a state of vitamin D deficiency.<\/jats:p>","DOI":"10.1038\/s41598-021-86730-0","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T10:09:07Z","timestamp":1618222147000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Short-range UV-LED irradiation in postmenopausal osteoporosis using ovariectomized mice"],"prefix":"10.1038","volume":"11","author":[{"given":"Satoshi","family":"Ochiai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Nishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitoshi","family":"Higuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daigo","family":"Morita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuya","family":"Makida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisuke","family":"Seki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihiro","family":"Ikuta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Imagama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,12]]},"reference":[{"key":"86730_CR1","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1007\/s00774-018-0916-1","volume":"37","author":"S Fujiwara","year":"2019","unstructured":"Fujiwara, S., Zhao, X., Teoh, C., Jaffe, D. 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