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Here, the biological characterization of these peptides was performed to engineer multifunctional peptides. Bioactive peptides were produced from mackerel byproducts via successive enzymatic hydrolysis reactions using subtilisin A and actinidin as microbial and herbal proteases. The antibacterial activity against both gram-negative and -positive food-borne pathogens, including<jats:italic>Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus<\/jats:italic>, and<jats:italic>Klebsiella pneumoniae<\/jats:italic>, as well as the inhibitory potential of angiotensin-converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV), was accessed in vitro. The synthesized peptides demonstrated multifunctional properties, which were further confirmed by in silico protocols. The ACE and DPP-IV inhibitory (IC<jats:sub>50<\/jats:sub>) values of P1, P2, and P3 were 0.92 and 0.87, 0.51 and 0.93, 0.78 and 1.16\u00a0mg\u00a0mL<jats:sup>\u22121<\/jats:sup>, respectively. 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