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In this study, we have investigated a panel of six host cell methylation markers (<jats:italic>GHSR, SST, ZIC1, ASCL1, LHX8, ST6GALNAC5<\/jats:italic>) in cervicovaginal secretions collected within the first part of the urine void (FVU) from a referral population. Cytology, histology, and HPV DNA genotyping results on paired FVU and cervical samples were available. Urinary median methylation levels from HR-HPV (n\u2009=\u200993) positive women were found to increase for all markers with severity of underlying disease. Significantly elevated levels were observed for <jats:italic>GHSR<\/jats:italic> and <jats:italic>LHX8<\/jats:italic> in relation to high-grade cervical intraepithelial neoplasia (CIN2\u2009+; n\u2009=\u200933), with area under de curve values of 0.80 (95% Confidence Interval (CI) 0.59\u20130.92) and 0.76 (95% CI 0.58\u20130.89), respectively. These findings are the first to support the assertion that methylation analysis of host cell genes is feasible in FVU and holds promise as molecular, triage strategy to discern low- from high-grade cervical disease in HR-HPV positive women. Molecular testing on FVU may serve to increase cervical cancer screening attendance in hard-to-reach populations whilst reducing loss to follow-up and await further optimization and validation studies.<\/jats:p>","DOI":"10.1038\/s41598-021-87329-1","type":"journal-article","created":{"date-parts":[[2021,4,12]],"date-time":"2021-04-12T10:09:07Z","timestamp":1618222147000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Triage of human papillomavirus infected women by methylation analysis in first-void urine"],"prefix":"10.1038","volume":"11","author":[{"given":"Severien","family":"Van Keer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annina P.","family":"van Splunter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade","family":"Pattyn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemie","family":"De Smet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sereina A.","family":"Herzog","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xaveer","family":"Van Ostade","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiebren A. 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Van Damme and A. Vorsters are co-founders of Novosanis (Belgium), a spin-off company of the University of Antwerp, and were minority shareholder until January 2019. R. D. M. Steenbergen has a minority stake in Self-screen B.V. (The Netherlands), a spin-off company of VU University Medical Center Amsterdam, which owns patents related to this work. The other authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"7862"}}