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Carcinogenic foodborne trematodes <jats:italic>Opisthorchis viverrini<\/jats:italic>, <jats:italic>Clonorchis sinensis,<\/jats:italic> and <jats:italic>O<\/jats:italic>. <jats:italic>felineus<\/jats:italic> might promote transmission and spread of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> infection in the definitive mammalian host, which in turn might contribute to the liver fluke-associated malignancy. Our objectives were to find out whether liver flukes <jats:italic>O<\/jats:italic>. <jats:italic>felineus<\/jats:italic>, <jats:italic>O<\/jats:italic>. <jats:italic>viverrini<\/jats:italic>, and <jats:italic>C<\/jats:italic>. <jats:italic>sinensis<\/jats:italic> are carriers of <jats:italic>Helicobacter pylori<\/jats:italic> and to determine whether <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> is present in feces, bile, and stomach samples from the experimentally infected hamsters. We found that liver flukes are not reservoirs of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>. Nevertheless, the prevalence of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> and the <jats:italic>H<\/jats:italic>. <jats:italic>pylori ureA<\/jats:italic> gene copy number were significantly elevated after the infection. Overall, although the liver flukes <jats:italic>O<\/jats:italic>. <jats:italic>felineus<\/jats:italic>, <jats:italic>C<\/jats:italic>. <jats:italic>sinensis<\/jats:italic>, and <jats:italic>O<\/jats:italic>. <jats:italic>viverrini<\/jats:italic> are not reservoirs of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>, the infection with the liver flukes significantly modifies the biliary and gut microbiota by increasing <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> abundance. This may be a feature of any liver fluke pathogenesis that have not previously been taken into account. Our findings appear to be novel in terms of comparative assessment of the host microbiota and <jats:italic>Helicobacter<\/jats:italic> abundance during epidemiologically important liver fluke infections.<\/jats:p>","DOI":"10.1038\/s41598-021-87446-x","type":"journal-article","created":{"date-parts":[[2021,4,8]],"date-time":"2021-04-08T10:13:19Z","timestamp":1617876799000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A comparative study of Helicobacter pylori infection in hamsters experimentally infected with liver flukes Opisthorchis felineus, Opisthorchis viverrini, or Clonorchis sinensis"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1054-2068","authenticated-orcid":false,"given":"Maria Y.","family":"Pakharukova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oxana","family":"Zaparina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung-Jong","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Banchob","family":"Sripa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viatcheslav A.","family":"Mordvinov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,4,8]]},"reference":[{"key":"87446_CR1","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1016\/j.ijpara.2013.07.007","volume":"43","author":"TN Petney","year":"2013","unstructured":"Petney, T. 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