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Different concentrations of methanolic extract (3.125 to 200\u00a0mg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) inhibited fungal biomass by 39\u201345%. Isolated\n                    <jats:italic>n-<\/jats:italic>\n                    hexane, chloroform and ethyl acetate fractions suppressed fungal biomass by 32\u201352%, 29\u201350% and 29\u201335%, respectively. Triterpenes lupeol and lupeol acetate\n                    <jats:bold>(1<\/jats:bold>\n                    ,\n                    <jats:bold>2<\/jats:bold>\n                    ) were isolated from n-hexane while betulin, \u03b2-sitosterol, \u03b2-amyrin, oleanolic acid (\n                    <jats:bold>3\u20136<\/jats:bold>\n                    ) were isolated from chloroform fraction. Vanillic acid, protocatechuic acid\n                    <jats:bold>,<\/jats:bold>\n                    kaempferol and quercetin\n                    <jats:bold>(7\u201310)<\/jats:bold>\n                    were isolated from the ethyl acetate fraction and identified using various spectroscopic techniques namely mass spectroscopy and NMR. Antifungal activity of different concentrations (0.0312 to 2\u00a0mg\u00a0mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) of the isolated compounds was evaluated and compared with the activity of a broad spectrum fungicide mancozeb. Different concentrations of mencozeb reduced fungal biomass by 83\u201385%. Among the isolated compounds lupeol acetate\n                    <jats:bold>(2<\/jats:bold>\n                    ) was found the highest antifungal against\n                    <jats:italic>M.<\/jats:italic>\n                    <jats:italic>phaseolina<\/jats:italic>\n                    followed by betulin\n                    <jats:bold>(3)<\/jats:bold>\n                    , vanillic acid (\n                    <jats:bold>7<\/jats:bold>\n                    ), protocatechuic acid\n                    <jats:bold>(8),<\/jats:bold>\n                    \u03b2-amyrin (\n                    <jats:bold>5<\/jats:bold>\n                    ) and oleanolic acid\n                    <jats:bold>(6)<\/jats:bold>\n                    resulting in 79\u201381%, 77\u201379%, 74\u201379%, 67\u201372%, 68\u201371% and 68\u201371%, respectively. Rest of the compounds also showed considerable antifungal activity and reduced\n                    <jats:italic>M.<\/jats:italic>\n                    <jats:italic>phaseolina<\/jats:italic>\n                    biomass by 41\u201364%.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-87725-7","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T06:10:06Z","timestamp":1618812606000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Lupeol acetate as a potent antifungal compound against opportunistic human and phytopathogenic mold Macrophomina phaseolina"],"prefix":"10.1038","volume":"11","author":[{"given":"Shabnam","family":"Javed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaid","family":"Mahmood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalid Mohammed","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satyajit D.","family":"Sarker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshad","family":"Javaid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iqra Haider","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7062-2823","authenticated-orcid":false,"given":"Amna","family":"Shoaib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"key":"87725_CR1","doi-asserted-by":"crossref","unstructured":"Shifa, H., Gopalakrishnan, C. & Velazhahan, R. 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