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The <jats:italic>TACC2<\/jats:italic> gene was known to be associated with tumors but the control of its expression is unclear. We have reported that activity of the intronic promoter p10 of <jats:italic>TACC2<\/jats:italic> in primary lesion of endometrial cancer is indicative of lymph node metastasis among a low-risk patient group. Here, we analyze the intronic promoter derived isoforms in JHUEM-1 endometrial cancer cells, and primary tissues of endometrial cancers and normal endometrium. Full-length cDNA amplicons are produced by long-range PCR and subjected to nanopore sequencing followed by computational error correction. We identify 16 stable, 4 variable, and 9 rare exons including 3 novel exons validated independently. All variable and rare exons reside N-terminally of the TACC domain and contribute to isoform variety. We found 240 isoforms as high-confidence, supported by more than 20 reads. The large number of isoforms produced from one minor promoter indicates the post-transcriptional complexity coupled with transcription at the <jats:italic>TACC2<\/jats:italic> locus in cancer and normal cells.<\/jats:p>","DOI":"10.1038\/s41598-021-88018-9","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T10:04:45Z","timestamp":1619777085000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Nanopore sequencing reveals TACC2 locus complexity and diversity of isoforms transcribed from an intronic promoter"],"prefix":"10.1038","volume":"11","author":[{"given":"Yosuke","family":"Ito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhisa","family":"Terao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Noma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michihira","family":"Tagami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiko","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihide","family":"Hayashizaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideya","family":"Kawaji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"key":"88018_CR1","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/0968-0004(96)10050-5","volume":"21","author":"RG Roeder","year":"1996","unstructured":"Roeder, R. 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