{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:06:53Z","timestamp":1778717213255,"version":"3.51.4"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T00:00:00Z","timestamp":1619049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the nutricosmetic effect of <jats:italic>Asparagus officinalis<\/jats:italic> extracts. The tip and spear of <jats:italic>A. officinalis<\/jats:italic> were successively extracted with 95% ethanol. The rutin, phenolic, and flavonoid contents of <jats:italic>A. officinalis<\/jats:italic> extracts were investigated. The antioxidant activities were determined by 2,2-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) and a ferric reducing antioxidant power assay. Matrix metalloproteinase-1 (MMP-1), elastase, and hyaluronidase inhibition were determined by in vitro enzyme reaction assay. The cytotoxicity was analyzed on peripheral blood mononuclear cellss. Findings revealed that drying temperature and drying duration had significant effects on the chemical composition and biological activity of <jats:italic>A. officinalis<\/jats:italic> extract. <jats:italic>A. officinalis<\/jats:italic> tips dried at 50\u00a0\u00b0C for 24\u00a0h contained the (significantly) highest flavonoid and rutin content. The most potent extract was from <jats:italic>A. officinalis<\/jats:italic> spears since it possessed the (significantly) highest MMP-1, elastase, and hyaluronidase inhibition rates of 83.4\u2009\u00b1\u20091.5%, 70.4\u2009\u00b1\u20094.1%, and 75.2\u2009\u00b1\u20091.0%, respectively. Interestingly, at the same concentration, the <jats:italic>A. officinalis<\/jats:italic> spear extract was more potent in MMP-1 inhibition than oleanolic acid and epigallocatechin gallate, the well-known natural MMP-1 inhibitors. The results show that <jats:italic>A. officinalis<\/jats:italic> extract is an attractive source of natural anti-skin-wrinkle ingredients.<\/jats:p>","DOI":"10.1038\/s41598-021-88340-2","type":"journal-article","created":{"date-parts":[[2021,4,22]],"date-time":"2021-04-22T10:10:11Z","timestamp":1619086211000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Nutricosmetic effects of Asparagus officinalis: a potent matrix metalloproteinase-1 inhibitor"],"prefix":"10.1038","volume":"11","author":[{"given":"Suwannee","family":"Sriyab","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nachtharinee","family":"Laosirisathian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanun","family":"Punyoyai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Songyot","family":"Anuchapreeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Singkome","family":"Tima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sawitree","family":"Chiampanichayakul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wantida","family":"Chaiyana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,4,22]]},"reference":[{"key":"88340_CR1","doi-asserted-by":"publisher","first-page":"03727","DOI":"10.1016\/j.jff.2019.103727","volume":"65","author":"N Wang","year":"2019","unstructured":"Wang, N., Liu, H., Li, Z., Lu, L. & Wang, C. The bioactive compounds and biological functions of Asparagus officinalis L.\u2014a review. J. Funct. Foods. 65, 03727. https:\/\/doi.org\/10.1016\/j.jff.2019.103727 (2019).","journal-title":"J. Funct. Foods."},{"key":"88340_CR2","doi-asserted-by":"publisher","unstructured":"Ntsoelinyane, P.M.A. & Mashele, S. Medicinal Properties of Selected Asparagus Species: A Review. https:\/\/doi.org\/10.5772\/intechopen.87048 (2019)","DOI":"10.5772\/intechopen.87048"},{"key":"88340_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/07373937.2020.1766484","volume":"38","author":"B Chitrakar","year":"2020","unstructured":"Chitrakar, B., Zhang, M. & Zhang, X. The determination of drying end-point for asparagus by-products with the use of LF-NMR spectra. Dry. Technol. 38, 1\u20137. https:\/\/doi.org\/10.1080\/07373937.2020.1766484 (2020).","journal-title":"Dry. Technol."},{"key":"88340_CR4","doi-asserted-by":"publisher","first-page":"326","DOI":"10.1016\/j.jfca.2010.10.008","volume":"24","author":"L Ferrara","year":"2011","unstructured":"Ferrara, L. et al. Nutritional values, metabolic profile and radical scavenging capacities of wild asparagus (A. acutifolius L.). J. Food Compos. Anal. 24, 326\u2013333. https:\/\/doi.org\/10.1016\/j.jfca.2010.10.008 (2011).","journal-title":"J. Food Compos. Anal."},{"key":"88340_CR5","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.foodchem.2005.03.033","volume":"97","author":"W Peschel","year":"2006","unstructured":"Peschel, W. et al. An industrial approach in the search of natural antioxidants from vegetable and fruit wastes. Food Chem. 97, 137\u2013150. https:\/\/doi.org\/10.1016\/j.foodchem.2005.03.033 (2006).","journal-title":"Food Chem."},{"key":"88340_CR6","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1016\/j.indcrop.2018.06.029","volume":"122","author":"I Chiocchio","year":"2018","unstructured":"Chiocchio, I. et al. Screening of a hundred plant extracts as tyrosinase and elastase inhibitors, two enzymatic targets of cosmetic interest. Ind. Crops. Prod. 122, 498\u2013505. https:\/\/doi.org\/10.1016\/j.indcrop.2018.06.029 (2018).","journal-title":"Ind. Crops. Prod."},{"key":"88340_CR7","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1111\/jfbc.12084","volume":"38","author":"W Zhang","year":"2014","unstructured":"Zhang, W., Wu, W., Wang, Q., Chen, Y. & Yue, G. The juice of asparagus by-product exerts hypoglycemic activity in streptozotocin-induced diabetic rats. J. Food Biochem. 38, 509\u2013517. https:\/\/doi.org\/10.1111\/jfbc.12084 (2014).","journal-title":"J. Food Biochem."},{"issue":"5","key":"88340_CR8","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.1021\/acs.jafc.8b05993","volume":"67","author":"Q Yu","year":"2019","unstructured":"Yu, Q., Li, J. & Fan, L. Effect of drying methods on the microstructure, bioactivity substances, and antityrosinase activity of Asparagus stems. J. Agric. Food Chem. 67(5), 1537\u20131545. https:\/\/doi.org\/10.1021\/acs.jafc.8b05993 (2019).","journal-title":"J. Agric. Food Chem."},{"issue":"5","key":"88340_CR9","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/S0023-6438(03)00046-X","volume":"36","author":"C Nindo","year":"2003","unstructured":"Nindo, C., Sun, T., Wang, S. W., Tang, J. & Powers, J. R. Evaluation of drying technologies for retention of physical quality and antioxidants in asparagus (Asparagus officinalis, L.). LWT Food Sci. Technol. 36(5), 507\u2013516. https:\/\/doi.org\/10.1016\/S0023-6438(03)00046-X (2003).","journal-title":"LWT Food Sci. Technol."},{"issue":"3","key":"88340_CR10","doi-asserted-by":"publisher","first-page":"171","DOI":"10.17221\/149\/2008-CJFS","volume":"27","author":"S Joki\u0107","year":"2009","unstructured":"Joki\u0107, S. et al. Influence of drying procedure on colour and rehydration characteristic of wild asparagus. Czech J. Food Sci. 27(3), 171\u2013177. https:\/\/doi.org\/10.17221\/149\/2008-CJFS (2009).","journal-title":"Czech J. Food Sci."},{"key":"88340_CR11","doi-asserted-by":"publisher","first-page":"392","DOI":"10.1016\/j.carbpol.2011.07.068","volume":"87","author":"Q Zhao","year":"2012","unstructured":"Zhao, Q. et al. In vitro antioxidant and antitumor activities of polysaccharides extracted from Asparagus officinalis. Carbohydr. Polym. 87, 392\u2013396. https:\/\/doi.org\/10.1016\/j.carbpol.2011.07.068 (2012).","journal-title":"Carbohydr. Polym."},{"key":"88340_CR12","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.foodchem.2010.12.102","volume":"127","author":"BS Wang","year":"2011","unstructured":"Wang, B. S. et al. Antioxidant and antityrosinase activity of aqueous extracts of green asparagus. Food Chem. 127, 141\u2013146. https:\/\/doi.org\/10.1016\/j.foodchem.2010.12.102 (2011).","journal-title":"Food Chem."},{"key":"88340_CR13","first-page":"373","volume":"45","author":"A Esmaeili","year":"2016","unstructured":"Esmaeili, A., Taha, R., Mohajer, S. & Banisalam, B. In vitro regeneration and comparison of phenolic content, antioxidant and antityrosinase activity of in vivo and in vitro grown Asparagus officinalis. Sains Malays. 45, 373\u2013381 (2016).","journal-title":"Sains Malays."},{"key":"88340_CR14","doi-asserted-by":"publisher","first-page":"108604","DOI":"10.1016\/j.lwt.2019.108604","volume":"117","author":"R Kaur","year":"2019","unstructured":"Kaur, R., Kaur, K. & Ahluwalia, P. Effect of drying temperatures and storage on chemical and bioactive attributes of dried tomato and sweet pepper. LWT Food Sci. Technol. 117, 108604. https:\/\/doi.org\/10.1016\/j.lwt.2019.108604 (2019).","journal-title":"LWT Food Sci. Technol."},{"key":"88340_CR15","doi-asserted-by":"publisher","first-page":"2995","DOI":"10.1021\/jf903655a","volume":"58","author":"H Lu","year":"2010","unstructured":"Lu, H. et al. Using neural networks to estimate the losses of ascorbic acid, total phenols, flavonoid, and antioxidant activity in asparagus during thermal treatments. J. Agric. Food Chem. 58, 2995\u20133001. https:\/\/doi.org\/10.1021\/jf903655a (2010).","journal-title":"J. Agric. Food Chem."},{"key":"88340_CR16","doi-asserted-by":"publisher","first-page":"127429","DOI":"10.1016\/j.foodchem.2020.127429","volume":"332","author":"M Li","year":"2020","unstructured":"Li, M. et al. Effect of thermal processing on free and bound phenolic compounds and antioxidant activities of hawthorn. Food Chem. 332, 127429. https:\/\/doi.org\/10.1016\/j.foodchem.2020.127429 (2020).","journal-title":"Food Chem."},{"key":"88340_CR17","doi-asserted-by":"publisher","first-page":"108830","DOI":"10.1016\/j.lwt.2019.108830","volume":"118","author":"S Kayacan","year":"2019","unstructured":"Kayacan, S. et al. Effect of different drying methods on total bioactive compounds, phenolic profile, in vitro bioaccessibility of phenolic and HMF formation of persimmon. LWT 118, 108830. https:\/\/doi.org\/10.1016\/j.lwt.2019.108830 (2019).","journal-title":"LWT"},{"key":"88340_CR18","doi-asserted-by":"publisher","first-page":"77","DOI":"10.17221\/168\/2019-CJFS(2020)","volume":"38","author":"M Zhang","year":"2019","unstructured":"Zhang, M. et al. A targeted analysis of flavonoids in asparagus using the UPLC-MS technique. Czech J. Food Sci. 38, 77\u201383. https:\/\/doi.org\/10.17221\/168\/2019-CJFS(2020) (2019).","journal-title":"Czech J. Food Sci."},{"key":"88340_CR19","doi-asserted-by":"publisher","DOI":"10.1007\/s12161-017-1074-0","author":"Y Ku","year":"2017","unstructured":"Ku, Y. et al. Detection of bioactive compounds in organically and conventionally grown asparagus spears. Food Anal. Methods https:\/\/doi.org\/10.1007\/s12161-017-1074-0 (2017).","journal-title":"Food Anal. Methods"},{"key":"88340_CR20","doi-asserted-by":"publisher","first-page":"215","DOI":"10.4103\/0973-7847.70921","volume":"4","author":"JS Negi","year":"2010","unstructured":"Negi, J. S., Singh, P., Joshi, G. P., Rawat, M. S. & Bisht, V. K. Chemical constituents of asparagus. Pharmacogn. Rev. 4, 215\u2013220. https:\/\/doi.org\/10.4103\/0973-7847.70921 (2010).","journal-title":"Pharmacogn. Rev."},{"key":"88340_CR21","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.foodchem.2007.03.048","volume":"105","author":"T Sun","year":"2007","unstructured":"Sun, T., Powers, J. R. & Tang, J. Evaluation of the antioxidant activity of asparagus, broccoli and their juices. Food Chem. 105, 101\u2013106. https:\/\/doi.org\/10.1016\/j.foodchem.2007.03.048 (2007).","journal-title":"Food Chem."},{"key":"88340_CR22","doi-asserted-by":"publisher","first-page":"103727","DOI":"10.1016\/j.jff.2019.103727","volume":"65","author":"Q Guo","year":"2020","unstructured":"Guo, Q. et al. The bioactive compounds and biological functions of Asparagus officinalis L.\u2014a review. J. Funct. Foods 65, 103727. https:\/\/doi.org\/10.1016\/j.jff.2019.103727 (2020).","journal-title":"J. Funct. Foods"},{"key":"88340_CR23","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1080\/003655102317475498","volume":"62","author":"A Janaszewska","year":"2002","unstructured":"Janaszewska, A. & Bartosz, G. Assay of total antioxidant capacity: Comparison of four methods as applied to human blood plasma. Scand. J. Clin. Lab Investig. 62, 231\u2013236. https:\/\/doi.org\/10.1080\/003655102317475498 (2002).","journal-title":"Scand. J. Clin. Lab Investig."},{"key":"88340_CR24","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/j.supflu.2015.02.014","volume":"100","author":"M Solana","year":"2015","unstructured":"Solana, M., Boschiero, I., Dall\u2019Acqua, S. & Bertucco, A. A comparison between supercritical fluid and pressurized liquid extraction methods for obtaining phenolic compounds from Asparagus officinalis L.. J. Supercrit. Fluids 100, 201\u2013208. https:\/\/doi.org\/10.1016\/j.supflu.2015.02.014 (2015).","journal-title":"J. Supercrit. Fluids"},{"key":"88340_CR25","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1080\/10286020.2016.1206529","volume":"19","author":"XM Li","year":"2017","unstructured":"Li, X. M. et al. Two new phenolic compounds and antitumor activities of asparinin A from Asparagus officinalis. J. Asian Nat. Prod. Res. 19, 164\u2013171. https:\/\/doi.org\/10.1080\/10286020.2016.1206529 (2017).","journal-title":"J. Asian Nat. Prod. Res."},{"key":"88340_CR26","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1021\/jf803537k","volume":"57","author":"JK Moon","year":"2009","unstructured":"Moon, J. K. & Shibamoto, T. Antioxidant assays for plant and food components. J. Agric. Food Chem. 57, 1655\u20131666. https:\/\/doi.org\/10.1021\/jf803537k (2009).","journal-title":"J. Agric. Food Chem."},{"key":"88340_CR27","doi-asserted-by":"publisher","first-page":"767","DOI":"10.1124\/jpet.103.059220","volume":"308","author":"S Ahmed","year":"2004","unstructured":"Ahmed, S., Wang, N., Lalonde, M., Goldberg, V. M. & Haqqi, T. M. Green tea polyphenol epigallocatechin-3-gallate (EGCG) differentially inhibits interleukin-1\u03b2-induced expression of matrix metalloproteinase-1 and -13 in human chondrocytes. J. Pharmacol. Exp. Ther. 308, 767\u2013773. https:\/\/doi.org\/10.1124\/jpet.103.059220 (2004).","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"88340_CR28","doi-asserted-by":"publisher","first-page":"197","DOI":"10.4196\/kjpp.2017.21.2.197","volume":"21","author":"DG Kang","year":"2017","unstructured":"Kang, D. G. et al. Effect of oleanolic acid on the activity, secretion and gene expression of matrix metalloproteinase-3 in articular chondrocytes in vitro and the production of matrix metalloproteinase-3 in vivo. Korean J. Physiol. Pharmacol. 21, 197. https:\/\/doi.org\/10.4196\/kjpp.2017.21.2.197 (2017).","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"88340_CR29","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.jgr.2017.11.003","volume":"42","author":"SY Lee","year":"2018","unstructured":"Lee, S. Y. Synergistic effect of maclurin on ginsenoside compound K induced inhibition of the transcriptional expression of matrix metalloproteinase-1 in HaCaT human keratinocyte cells. J. Ginseng Res. 42, 229. https:\/\/doi.org\/10.1016\/j.jgr.2017.11.003 (2018).","journal-title":"J. Ginseng Res."},{"key":"88340_CR30","unstructured":"Organizaci\u00f3n Mundial de la Salud, World Health Organization & \u015awiatowa Organizacja Zdrowia. WHO guidelines on good agricultural and collection practices [GACP] for medicinal plants. World Health Organization (2003)."},{"key":"88340_CR31","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1111\/j.1365-2621.1999.tb15876.x","volume":"64","author":"T Fuleki","year":"1999","unstructured":"Fuleki, T. Rutin, the main component of surface deposits on pickled green asparagus. J. Food Sci. 64, 252\u2013254. https:\/\/doi.org\/10.1111\/j.1365-2621.1999.tb15876.x (1999).","journal-title":"J. Food Sci."},{"key":"88340_CR32","doi-asserted-by":"publisher","first-page":"C703","DOI":"10.1111\/j.1750-3841.2010.01824.x","volume":"75","author":"E Lee","year":"2010","unstructured":"Lee, E., Yoo, K. & Patil, B. Development of a rapid HPLC-UV method for simultaneous quantification of protodioscin and rutin in white and green asparagus spears. J. Food Sci. 75, C703-709. https:\/\/doi.org\/10.1111\/j.1750-3841.2010.01824.x (2010).","journal-title":"J. Food Sci."},{"key":"88340_CR33","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.3390\/nu9101105","volume":"9","author":"W Chaiyana","year":"2017","unstructured":"Chaiyana, W. et al. Inhibition of 5\u03b1-reductase, IL-6 secretion, and oxidation process of equisetum debile Roxb ex vaucher extract as functional food and nutraceuticals ingredients. Nutrients 9, 1105. https:\/\/doi.org\/10.3390\/nu9101105 (2017).","journal-title":"Nutrients"},{"key":"88340_CR34","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/s0076-6879(99)99017-1","volume":"299","author":"VL Singleton","year":"1999","unstructured":"Singleton, V. L., Orthofer, R. & Lamuela-Ravent\u00f3s, R. M. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 299, 152\u2013178. https:\/\/doi.org\/10.1016\/s0076-6879(99)99017-1 (1999).","journal-title":"Methods Enzymol."},{"key":"88340_CR35","doi-asserted-by":"publisher","first-page":"1923","DOI":"10.3390\/molecules25081923","volume":"25","author":"W Chaiyana","year":"2020","unstructured":"Chaiyana, W. et al. Chemical constituents, antioxidant, anti-MMPs, and anti-hyaluronidase activities of Thunbergia laurifolia Lindl. leaf extracts for skin aging and skin damage prevention. Molecules 25, 1923. https:\/\/doi.org\/10.3390\/molecules25081923 (2020).","journal-title":"Molecules"},{"key":"88340_CR36","doi-asserted-by":"publisher","first-page":"178","DOI":"10.38212\/2224-6614.2748","volume":"10","author":"CC Chang","year":"2002","unstructured":"Chang, C. C., Yang, M. H., Wen, H. M. & Chern, J. C. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal. 10, 178\u2013182. https:\/\/doi.org\/10.38212\/2224-6614.2748 (2002).","journal-title":"J. Food Drug Anal."},{"key":"88340_CR37","first-page":"465","volume":"56","author":"RD Petry","year":"2001","unstructured":"Petry, R. D., Ortega, G. G. & Silva, W. B. Flavonoid content assay: influence of the reagent concentration and reaction time on the spectrophotometric behavior of the aluminium chloride\u2013flavonoid complex. Pharmazie 56, 465\u2013470 (2001).","journal-title":"Pharmazie"},{"key":"88340_CR38","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.indcrop.2018.10.081","volume":"127","author":"W Chaiyana","year":"2019","unstructured":"Chaiyana, W. et al. Ocimum sanctum Linn. as a natural source of skin anti-ageing compounds. Ind. Crops. Prod. 127, 217\u2013224. https:\/\/doi.org\/10.1016\/j.indcrop.2018.10.081 (2019).","journal-title":"Ind. Crops. Prod."},{"key":"88340_CR39","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1016\/j.foodchem.2006.07.034","volume":"103","author":"S Tachakittirungrod","year":"2007","unstructured":"Tachakittirungrod, S., Okonogi, S. & Chowwanapoonpohn, S. Study on antioxidant activity of certain plants in Thailand: mechanism of antioxidant action of guava leaf extract. Food Chem. 103, 381\u2013388. https:\/\/doi.org\/10.1016\/j.foodchem.2006.07.034 (2007).","journal-title":"Food Chem."},{"key":"88340_CR40","doi-asserted-by":"publisher","first-page":"144","DOI":"10.5582\/ddt.2011.v5.3.144","volume":"5","author":"K Saeio","year":"2011","unstructured":"Saeio, K., Chaiyana, W. & Okonogi, S. Antityrosinase and antioxidant activities of essential oils of edible Thai plants. Drug Discov. Ther. 5, 144\u2013149. https:\/\/doi.org\/10.5582\/ddt.2011.v5.3.144 (2011).","journal-title":"Drug Discov. Ther."},{"key":"88340_CR41","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1186\/1472-6882-9-27","volume":"9","author":"TSA Thring","year":"2009","unstructured":"Thring, T. S. A., Hili, P. & Naughton, D. P. Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complement Altern. Med. 9, 27. https:\/\/doi.org\/10.1186\/1472-6882-9-27 (2009).","journal-title":"BMC Complement Altern. Med."},{"key":"88340_CR42","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.3390\/molecules25051101","volume":"25","author":"N Laothaweerungsawat","year":"2020","unstructured":"Laothaweerungsawat, N., Sirithunyalug, J. & Chaiyana, W. Chemical compositions and anti-skin-ageing activities of Origanum vulgare L. essential oil from tropical and mediterranean region. Molecules 25, 1101. https:\/\/doi.org\/10.3390\/molecules25051101 (2020).","journal-title":"Molecules"},{"key":"88340_CR43","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/0142-9612(93)90055-7","volume":"14","author":"G Ciapetti","year":"1993","unstructured":"Ciapetti, G., Cenni, E., Pratelli, L. & Pizzoferrato, A. In vitro evaluation of cell\/biomaterial interaction by MTT assay. Biomaterials 14, 359\u2013364. https:\/\/doi.org\/10.1016\/0142-9612(93)90055-7 (1993).","journal-title":"Biomaterials"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-88340-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-88340-2","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-88340-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T07:17:15Z","timestamp":1670051835000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-88340-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,22]]},"references-count":43,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["88340"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-88340-2","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,22]]},"assertion":[{"value":"13 January 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 April 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 April 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"8772"}}