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Ejaculates (n\u2009=\u200982) collected from whitespotted bamboo sharks\n                    <jats:italic>Chiloscyllium plagiosum<\/jats:italic>\n                    (n\u2009=\u200919) were assessed and cold-stored raw or extended at 4\u00a0\u00b0C. Females (n\u2009=\u200920) were inseminated with fresh or 24\u201348\u00a0h cold-stored raw or extended semen and paternity of offspring determined with microsatellite markers. Insemination of females with fresh semen (n\u2009=\u200910) resulted in 80 hatchlings and 27.6% fertility. Insemination of females with semen cold-stored 24\u00a0h (n\u2009=\u20094) and 48\u00a0h (n\u2009=\u20091) semen resulted in 17 hatchlings and fertilization rates of 28.1% and 7.1% respectively. Two females inseminated with fresh\u00a0or cold-stored semen laid eggs that hatched from fertilization and parthenogenesis within the same clutch. Parthenogenesis rate for inseminated females was 0.71%. Results demonstrate artificial insemination with cold-stored semen can provide a strategy for transport of male genetics nationally and internationally, precluding the need to transport sharks. Production of parthenotes in the same clutch as sexually\u00a0fertilized eggs highlights the prevalence of parthenogenesis in whitespotted bamboo sharks and poses important considerations for population management.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-88568-y","type":"journal-article","created":{"date-parts":[[2021,5,12]],"date-time":"2021-05-12T19:04:01Z","timestamp":1620846241000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Artificial insemination and parthenogenesis in the whitespotted bamboo shark Chiloscyllium plagiosum"],"prefix":"10.1038","volume":"11","author":[{"given":"Jennifer T.","family":"Wyffels","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lance M.","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Bulman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ari","family":"Fustukjian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Hyatt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Feldheim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Penfold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,13]]},"reference":[{"key":"88568_CR1","doi-asserted-by":"publisher","unstructured":"Kyne, P.M. & Burgess, G. 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