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In this study, we isolated and genetically characterized\n                    <jats:italic>T. gondii<\/jats:italic>\n                    strains from domestic animals in Southern and coastal area of Tunisia. Blood, hearts and\/or brains were collected from 766 domestic animals (630 sheep and 136 free-range chickens). Strain isolation from these samples was performed using mouse bioassay and genotyping was carried out with a multiplex PCR technique using 15 microsatellite markers. Thirty viable strains of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    were successfully isolated from tissues of sheep (19\/142) and chickens (11\/33). In addition, 3 strains could be successfully genotyped from animal tissues for which mouse bioassay was unsuccessful. A large predominance of type II strains (n\u2009=\u200929) was found in the sampled regions, followed by type III (n\u2009=\u20093) and, for the first time in Tunisia, a single isolate of Africa 4 lineage from a sheep. Analyses of population genetics showed the presence of a divergent population of type II lineage in Tunisia, supporting limited recent migrations of strains between Tunisia and other countries of the world.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-88751-1","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T06:05:37Z","timestamp":1619676337000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["First isolation and genotyping of Toxoplasma gondii strains from domestic animals in Tunisia"],"prefix":"10.1038","volume":"11","author":[{"given":"Arwa","family":"Lachkhem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lokman","family":"Galal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibtissem","family":"Lahmar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Passebosc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Homayoun","family":"Riahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Plault","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Laure","family":"Dard\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lien","family":"Mercier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamouda","family":"Babba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"88751_CR1","first-page":"996E","volume":"13","author":"M Opsteegh","year":"2016","unstructured":"Opsteegh, M., Maas, M., Schares, G. & van der Giessen, J. 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