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The EQ group scored higher in nerve conduction studies, compared to vehicle-treated db\/db group. Morphology studies yielded similar results. To investigate the potential role of mitochondrial DNA (mtDNA) deletions in the observed effects of EQ, we measured total mtDNA deletion burden in the distal sciatic nerve. We observed an increase in total mtDNA deletion burden in vehicle-treated db\/db mice compared to db\/+\u2009mice that was partially prevented in db\/db-EQ treated animals. These results suggest that EQ treatment may exert a neuroprotective effect in diabetic neuropathy. The prevention of diabetes-induced mtDNA deletions may be a potential mechanism of the neuroprotective effects of EQ in diabetic neuropathy.<\/jats:p>","DOI":"10.1038\/s41598-021-89781-5","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T10:02:53Z","timestamp":1621850573000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ethoxyquin is neuroprotective and partially prevents somatic and autonomic neuropathy in db\/db mouse model of type 2 diabetes"],"prefix":"10.1038","volume":"11","author":[{"given":"Ying","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osefame","family":"Ewaleifoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josh","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruifa","family":"Mi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Hoke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Polydefkis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,24]]},"reference":[{"key":"89781_CR1","doi-asserted-by":"crossref","unstructured":"Dyck, P.J.,\u00a0Kratz, K.M.,\u00a0Karnes, J.L.,\u00a0Litchy, W.J.,\u00a0Klein, R.,\u00a0Pach, J.M.,\u00a0Wilson, D.M.,\u00a0O'Brien, P.C.,\u00a0Melton, L.J. 3rd,\u00a0Service, F.J. 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Dr. Hoke also is a founder and serves as the Chair of AxoProtego Therapeutics LLC's Scientific Advisory Board. This arrangement has been reviewed and approved by the Johns Hopkins University in accordance with its conflict-of-interest policies. No other competing interests exist for Y. L., M. P., Y. S., O. E., J. W., RF. M. or J. Z.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"10749"}}