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In recent years, dyes have become a major water pollutant used in the manufacture of paints and other production purposes. In this research, the potential of duckweed (<jats:italic>Lemna gibba<\/jats:italic>) plant was investigated spectrophotometrically as an obvious bioagent for the biological decolorization of the organic dye C.I. Basic Green 4 (Malachite Green, BG4). Photosynthetic efficiency analysis showed that the photosynthetic apparatus of <jats:italic>L. gibba<\/jats:italic> is very tolerant to BG4. Significant induction of reactive oxygen species (ROS) scavenging enzymes was observed after 24h of biodecolorization process in <jats:italic>L. gibba<\/jats:italic> treated with 15 and 30 mg\/l BG4. The experimental results showed that <jats:italic>L. gibba<\/jats:italic> has a strong ability to extract BG4 from contaminated water and the best results were obtained at 25\u201330\u00b0C and pH 8.0. 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