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Although anti-<jats:italic>C. albicans<\/jats:italic> mannan antibody levels are monitored for diagnostic purposes nothing is known about the qualitative distribution of these antibodies in terms of epitope specificity. We addressed this question using a bank of previously synthesized biotin sulfone tagged oligomannosides (BSTOs) of \u03b1 and \u03b2 anomery complemented with a synthetic \u03b2-mannotriose described as a protective epitope. The reactivity of these BSTOs was analyzed with IgM isotype monoclonal antibodies (MAbs) of known specificity, polyclonal sera from patients colonized or infected with <jats:italic>C. albicans<\/jats:italic>, and mannose binding lectin (MBL). Surface plasmon resonance (SPR) and multiple analyte profiling (MAP) were used. Both methods confirmed the usual reactivity of MAbs against either \u03b1 or \u03b2 linkages, excepted for MAb B6.1 (protective epitope) reacting with \u03b2-Man whereas the corresponding BSTO reacted with anti-\u03b1-Man. These results were confirmed in western blots with native <jats:italic>C. albicans<\/jats:italic> antigens. Using patients\u2019 sera in MAP, a significant correlation was observed between the detection of anti-mannan antibodies recognizing \u03b2- and \u03b1-Man epitopes and detection of antibodies against \u03b2-linked mannotriose suggesting that this epitope also reacts with human polyclonal antibodies of both specificities. By contrast, the reactivity of human sera with other \u03b1- and \u03b2-linked BSTOs clearly differed according to their colonized or infected status. In these cases, the establishment of an \u03b1\/\u03b2 ratio was extremely discriminant. Finally SPR with MBL, an important lectin of innate immunity to <jats:italic>C. albicans<\/jats:italic>, classically known to interact with \u03b1-mannose, also interacted in an unexpected way with the protective epitope. These cumulative data suggest that structure\/activity investigations of the finely tuned <jats:italic>C. albicans<\/jats:italic> anti-mannose immune response are worthwhile to increase our basic knowledge and for translation in medicine.<\/jats:p>","DOI":"10.1038\/s41598-021-90402-4","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T10:03:09Z","timestamp":1621850589000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Dissection of the anti-Candida albicans mannan immune response using synthetic oligomannosides reveals unique properties of \u03b2-1,2 mannotriose protective epitopes"],"prefix":"10.1038","volume":"11","author":[{"given":"Boualem","family":"Sendid","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Lecointe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayeul","family":"Collot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Marie","family":"Danz\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Damiens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Drucbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Fradin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Vilcot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Grenouillet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faustine","family":"Dubar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"de Ruyck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir","family":"Jawhara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Maurice","family":"Mallet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Poulain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,24]]},"reference":[{"key":"90402_CR1","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/S1471-4906(01)01890-7","volume":"22","author":"J Axford","year":"2001","unstructured":"Axford, J. 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