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The role of oral sex, rimming, and kissing on <jats:italic>M. genitalium<\/jats:italic> transmission in MSM is unclear. We created four deterministic susceptible-infectious-susceptible epidemic models to examine the role that different sexual behaviours play in transmitting <jats:italic>M. genitalium<\/jats:italic> at the oropharynx, urethra anorectum among men who have sex with men in Australia. Our results suggest that oral and anal sex without other sexual practices (model 1) replicate well single site infection at the oropharynx, urethra and anorectum and also multi-site infection. If kissing or rimming are added to model 1 (i.e., model 2\u20134) no substantial improvements in the calibration of the models occur. Model 1 estimates that 3.4% of infections occur at the oropharynx, 34.8% at the urethra and 61.8% at the anorectum. Model 1 also estimates that the proportion of incident <jats:italic>M. genitalium<\/jats:italic> transmitted by anal sex was 82.4%, and by oral sex was about 17.6%. Our findings could provide an enhanced understanding of <jats:italic>M. genitalium<\/jats:italic> transmission in MSM, thus providing insights into what sexual practices contribute most to transmission.<\/jats:p>","DOI":"10.1038\/s41598-021-90627-3","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T10:12:23Z","timestamp":1622110343000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Modelling the multiple anatomical site transmission of Mycoplasma genitalium among men who have sex with men in Australia"],"prefix":"10.1038","volume":"11","author":[{"given":"Xianglong","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona S.","family":"Bradshaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric P. F.","family":"Chow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason J.","family":"Ong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane S.","family":"Hocking","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher K.","family":"Fairley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,27]]},"reference":[{"key":"90627_CR1","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2019-054310","author":"RL Latimer","year":"2020","unstructured":"Latimer, R. L. et al. Prevalence of Mycoplasma genitalium by anatomical site in men who have sex with men: A systematic review and meta-analysis. Sex Transm. Infect. https:\/\/doi.org\/10.1136\/sextrans-2019-054310 (2020).","journal-title":"Sex Transm. Infect."},{"key":"90627_CR2","doi-asserted-by":"publisher","DOI":"10.1016\/s1473-3099(20)30154-7","author":"DA Machalek","year":"2020","unstructured":"Machalek, D. A. et al. Prevalence of mutations associated with resistance to macrolides and fluoroquinolones in Mycoplasma genitalium: A systematic review and meta-analysis. Lancet Infect. Dis. https:\/\/doi.org\/10.1016\/s1473-3099(20)30154-7 (2020).","journal-title":"Lancet Infect. Dis."},{"key":"90627_CR3","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1038\/nrurol.2016.268","volume":"14","author":"M Unemo","year":"2017","unstructured":"Unemo, M. & Jensen, J. S. Antimicrobial-resistant sexually transmitted infections: Gonorrhoea and Mycoplasma genitalium. Nat. Rev. Urol. 14, 139\u2013152. https:\/\/doi.org\/10.1038\/nrurol.2016.268 (2017).","journal-title":"Nat. Rev. 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