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We examined comprehensive profiles of senescent BECs and its contribution to the pathogenesis of PBC taking advantage of microarray analysis. cDNA microarray analysis revealed that 1841 genes including <jats:italic>CCL2<\/jats:italic>, <jats:italic>IFIT3, CPQ<\/jats:italic> were commonly up-regulated in senescent BECs cultured in serum depleted media or media with glycochenodeoxycholic acid<jats:italic>.<\/jats:italic> Knockdown of <jats:italic>IFIT3<\/jats:italic> significantly suppressed cellular senescence (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) and significantly increased apoptosis (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) in BECs treated with serum depletion or glycochenodeoxycholic acid. Significantly increased expression of IFIT3 was seen in senescent BECs in small bile ducts showing cholangitis and in ductular reactions in PBC, compared to control livers (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). An inadequate response to UDCA was inversely correlated to the increased expression of IFIT3 in small bile duct in PBC (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). In conclusion, the expression of various genes related to immunity and inflammation including SASPs were increased in senescent BECs. Upregulated IFIT3 in senescent BECs may be associated with the pathogenesis of PBC and may be a possible therapeutic target in PBC.<\/jats:p>","DOI":"10.1038\/s41598-021-91016-6","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T15:32:54Z","timestamp":1622561574000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Interferon-induced protein with tetratricopeptide repeats 3 may be a key factor in primary biliary cholangitis"],"prefix":"10.1038","volume":"11","author":[{"given":"Motoko","family":"Sasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasunori","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuni","family":"Nakanuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,1]]},"reference":[{"key":"91016_CR1","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1056\/NEJMra043898","volume":"353","author":"M Kaplan","year":"2005","unstructured":"Kaplan, M. & Gershwin, M. 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