{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T02:06:24Z","timestamp":1790388384189,"version":"4.1.0"},"reference-count":46,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T00:00:00Z","timestamp":1623369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Viral interference is a common occurrence that has been reported in cell culture in many cases. In the present study, viral interference between two capripox viruses (sheeppox SPPV and lumpy skin disease virus LSDV in cattle) with Rift Valley fever virus (RVFV) was investigated in vitro and in their natural hosts, sheep and cattle. A combination of SPPV\/RVFV and LSDV\/RVFV was used to co-infect susceptible cells and animals to detect potential competition. In-vitro interference was evaluated by estimating viral infectivity and copies of viral RNA by a qPCR during three serial passages in cell cultures, whereas in-vivo interference was assessed through antibody responses to vaccination. When lamb testis primary cells were infected with the mixture of capripox and RVFV, the replication of both SPPV and LSDV was inhibited by RVFV. In animals, SPPV\/RVFV or LSDV\/RVFV combinations inhibited the replication SPPV and LSDV and the antibody response following vaccination. The combined SPPV\/RVFV did not protect sheep after challenging with the virulent strain of SPPV and the LSDV\/RVFV did not induce interferon Gamma to LSDV, while immunological response to RVFV remain unaffected. Our goal was to assess this interference response to RVFV\/capripoxviruses\u2019 coinfection in order to develop effective combined live-attenuated vaccines as a control strategy for RVF and SPP\/LSD diseases. Our findings indicated that this approach was not suitable for developing a combined SPPV\/LSDV\/RVFV vaccine candidate because of interference of replication and the immune response among these viruses.<\/jats:p>","DOI":"10.1038\/s41598-021-91926-5","type":"journal-article","created":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T10:05:00Z","timestamp":1623405900000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In-vitro and in-vivo study of the interference between Rift Valley fever virus (clone 13) and Sheeppox\/Lumpy Skin disease viruses"],"prefix":"10.1038","volume":"11","author":[{"given":"N.","family":"Safini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Bamouh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Hamdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Jazouli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. O.","family":"Tadlaoui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"El Harrak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,11]]},"reference":[{"issue":"6","key":"91926_CR1","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1051\/vetres\/2010033","volume":"41","author":"M Pepin","year":"2010","unstructured":"Pepin, M., Bouloy, M., Bird, B. H., Kemp, A. & Paweska, J. Rift Valley fever virus (Bunyaviridae: Phlebovirus): An update on pathogenesis, molecular epidemiology, vectors, diagnostics and prevention. Vet. Res. 41(6), 61 (2010).","journal-title":"Vet. Res."},{"key":"91926_CR2","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1099\/0022-1317-71-3-501","volume":"71","author":"RM Elliott","year":"1990","unstructured":"Elliott, R. M. Molecular biology of the Bunyaviridae. J. Gen. Virol. 71, 501\u2013522 (1990).","journal-title":"J. Gen. Virol."},{"key":"91926_CR3","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.antiviral.2014.06.009","volume":"109","author":"ESM Tuppurainen","year":"2014","unstructured":"Tuppurainen, E. S. M. et al. Characterization of sheep pox virus vaccine for cattle against lumpy skin disease virus. Antiviral Res. 109, 1\u20136 (2014).","journal-title":"Antiviral Res."},{"key":"91926_CR4","doi-asserted-by":"publisher","first-page":"a010199","DOI":"10.1101\/cshperspect.a010199","volume":"5","author":"B Moss","year":"2013","unstructured":"Moss, B. Poxvirus DNA replication. Cold Spring Harb. Perspect. Biol. 5, a010199 (2013).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"91926_CR5","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7643-7557-7_8","volume-title":"Poxviruses. Birkh\u00e4user Advances in Infectious Diseases","author":"A Diallo","year":"2007","unstructured":"Diallo, A. & Viljoen, G. J. Genus Capripoxvirus. In Poxviruses. Birkh\u00e4user Advances in Infectious Diseases (eds Mercer, A. A., Schmidt, A. & Weber, O.) (Birkh\u00e4user, Basel, 2007). https:\/\/doi.org\/10.1007\/978-3-7643-7557-7_8."},{"key":"91926_CR6","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.prevetmed.2017.09.003","volume":"147","author":"W Molla","year":"2017","unstructured":"Molla, W., de Jong, M. C. M., Gari, G. & Frankena, K. Economic impact of lumpy skin disease and cost effectiveness of vaccination for the control of outbreaks in Ethiopia. Prev. Vet. Med. 147, 100\u2013107 (2017).","journal-title":"Prev. Vet. Med."},{"key":"91926_CR7","doi-asserted-by":"publisher","first-page":"1397","DOI":"10.1056\/NEJM199411243312101","volume":"331","author":"H Peltola","year":"1994","unstructured":"Peltola, H. et al. The elimination of indigenous Measles, Mumps, and Rubella from Finland by a 12-year, two-dose vaccination program. N. Engl. J. Med. 331, 1397\u20131402 (1994).","journal-title":"N. Engl. J. Med."},{"key":"91926_CR8","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.trivac.2015.03.004","volume":"4","author":"F Fakri","year":"2015","unstructured":"Fakri, F. et al. Development and field application of a new combined vaccine against Peste des Petits Ruminants and Sheep Pox. Trials Vaccinol. 4, 33\u201337 (2015).","journal-title":"Trials Vaccinol."},{"issue":"3","key":"91926_CR9","first-page":"173","volume":"5","author":"OY Abdelmagid","year":"2004","unstructured":"Abdelmagid, O. Y., Larson, L., Payne, L., Tubbs, A., Wasmoen, T. & Schultz, R. Evaluation of the efficacy and duration of immunity of a canine combination vaccine against virulent parvovirus, infectious canine hepatitis virus, and distemper virus experimental challenges. Vet. Ther. 5(3), 173\u2013186 (2004).","journal-title":"Vet. Ther."},{"key":"91926_CR10","doi-asserted-by":"publisher","first-page":"5489","DOI":"10.1038\/s41598-020-62453-6","volume":"10","author":"YB Ngoshe","year":"2015","unstructured":"Ngoshe, Y. B., Avenant, A., Rostal, M. K., et al. Patterns of Rift Valley fever virus seropositivity in domestic ruminants in central South Africa four years after a large outbreak. Sci. Rep. 10, 5489. https:\/\/doi.org\/10.1038\/s41598-020-62453-6 (2020).","journal-title":"Sci. Rep."},{"key":"91926_CR11","volume-title":"Cell culture for biochemists","author":"R Adams","year":"1990","unstructured":"Adams, R. & Roger, L. P. Cell culture for biochemists (Elsevier\/North-Holland Biomedical Press, 1990)."},{"key":"91926_CR12","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","volume":"27","author":"LJ Reed","year":"1938","unstructured":"Reed, L. J. & Muench, H. A simple method of estimating fifty per cent endpoints. Am. J. Epidemiol. 27, 493\u2013497 (1938).","journal-title":"Am. J. Epidemiol."},{"key":"91926_CR13","doi-asserted-by":"publisher","first-page":"6054","DOI":"10.1128\/JVI.76.12.6054-6061.2002","volume":"76","author":"ER Tulman","year":"2002","unstructured":"Tulman, E. R. et al. The genomes of Sheeppox and Goatpox viruses. J. Virol. 76, 6054\u20136061 (2002).","journal-title":"J. Virol."},{"key":"91926_CR14","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1089\/vbz.2009.0205","volume":"10","author":"N Busquets","year":"2010","unstructured":"Busquets, N. et al. Experimental infection of young adult european breed sheep with Rift Valley fever virus field isolates. Vector-Borne Zoonotic Dis. 10, 689\u2013696 (2010).","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"91926_CR15","unstructured":"Fassi-Fehri, M. et al. Etude experimentale de l\u2019immunite anticlaveleuse post-vaccinale. Annales de Recherches V\u00e9t\u00e9rinaires, INRA Editions, 15(1), 59\u201364 (1984)."},{"key":"91926_CR16","doi-asserted-by":"publisher","first-page":"730","DOI":"10.4269\/ajtmh.14-0363","volume":"92","author":"GO Muga","year":"2015","unstructured":"Muga, G. O., Onyango-Ouma, W., Sang, R. & Affognon, H. Review article: Sociocultural and economic dimensions of Rift Valley Fever. Am. J. Trop. Med. Hyg. 92, 730\u2013738 (2015).","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"91926_CR17","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1111\/j.1865-1682.2011.01242.x","volume":"59","author":"ESM Tuppurainen","year":"2012","unstructured":"Tuppurainen, E. S. M. & Oura, C. A. L. Review: Lumpy skin disease: An emerging threat to Europe, the Middle East and Asia. Transbound. Emerg. Dis. 59, 40\u201348 (2012).","journal-title":"Transbound. Emerg. Dis."},{"key":"91926_CR18","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1111\/tbed.12444","volume":"64","author":"ESM Tuppurainen","year":"2017","unstructured":"Tuppurainen, E. S. M. et al. Review: Capripoxvirus diseases: current status and opportunities for control. Transbound. Emerg. Dis. 64, 729\u2013745 (2017).","journal-title":"Transbound. Emerg. Dis."},{"issue":"1","key":"91926_CR19","doi-asserted-by":"publisher","first-page":"e00298-16","DOI":"10.1128\/CVI.00298-16","volume":"24","author":"KB Lauer","year":"2017","unstructured":"Lauer, K. B., Borrow, R. & Blanchard, T. J. Multivalent and multipathogen viral vector vaccines. Clin. Vaccine Immunol. 24(1), e00298-16. https:\/\/doi.org\/10.1128\/CVI.00298-16 (2017).","journal-title":"Clin. Vaccine Immunol."},{"key":"91926_CR20","doi-asserted-by":"publisher","first-page":"1842","DOI":"10.1128\/CVI.00220-10","volume":"17","author":"RK Soi","year":"2010","unstructured":"Soi, R. K. et al. Protection of sheep against Rift Valley fever virus and sheep poxvirus with a recombinant capripoxvirus vaccine. Clin. Vaccine Immunol. 17, 1842\u20131849 (2010).","journal-title":"Clin. Vaccine Immunol."},{"key":"91926_CR21","doi-asserted-by":"publisher","first-page":"256","DOI":"10.3389\/fvets.2020.00256","volume":"7","author":"DB Wallace","year":"2020","unstructured":"Wallace, D. B. et al. Protection of cattle elicited using a bivalent Lumpy skin disease virus-vectored recombinant Rift Valley fever vaccine. Front. Vet. Sci. 7, 256 (2020).","journal-title":"Front. Vet. Sci."},{"key":"91926_CR22","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1007\/BF02908284","volume":"5","author":"F Dianzani","year":"1975","unstructured":"Dianzani, F. Viral interference and interferon. Ric. Clin. Lab. 5, 196\u2013213 (1975).","journal-title":"Ric. Clin. Lab."},{"key":"91926_CR23","doi-asserted-by":"publisher","first-page":"873404","DOI":"10.1155\/2015\/873404","volume":"2015","author":"JS Salas-Benito","year":"2015","unstructured":"Salas-Benito, J. S. & De Nova-Ocampo, M. Viral interference and persistence in mosquito-borne Flaviviruses. J. Immunol. Res. 2015, 873404 (2015). https:\/\/doi.org\/10.1155\/2015\/873404.","journal-title":"J. Immunol. Res."},{"key":"91926_CR24","volume-title":"Fields virology","author":"BN Fields","year":"2007","unstructured":"Fields, B. N., Knipe, D. M. & Howley, P. M. Fields virology (Wolters Kluwer Health\/Lippincott Williams & Wilkins, Philadelphia, 2007)."},{"key":"91926_CR25","first-page":"227","volume":"11","author":"D Taranov","year":"2014","unstructured":"Taranov, D., Yershebulov, Z., Amanova, Z., Bulatov, Y., Barakbayev, K., Abduraimov, Y., Kondibayeva, Zh., Znugunissov, K. & Sansyzbay, A. Study of cultural characteristics and interference of Peste des Petit ruminants virus and sheep pox virus in co-culture. Life Sci. J. 11, 227\u2013231 (2014).","journal-title":"Life Sci. J."},{"key":"91926_CR26","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1007\/s11259-006-3259-7","volume":"30","author":"T\u00c7 O\u011fuzo\u011flu","year":"2006","unstructured":"O\u011fuzo\u011flu, T. \u00c7. et al. A Sheeppox virus outbreak in central Turkey in 2003: isolation and identification of Capripoxvirus ovis. Vet. Res. Commun. 30, 965\u2013971 (2006).","journal-title":"Vet. Res. Commun."},{"issue":"2","key":"91926_CR27","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1177\/104063879000200205","volume":"2","author":"JA House","year":"1990","unstructured":"House, J. A., Wilson, T. M., el Nakashly, S., Karim, I. A., Ismail, I., el Danaf, N., Moussa, A. M. & Ayoub, N. N. The isolation of lumpy skin disease virus and bovine herpesvirus-4 from cattle in Egypt. J. Vet. Diagn. Invest. 2(2), 111\u2013115. https:\/\/doi.org\/10.1177\/104063879000200205 (1990).","journal-title":"J. Vet. Diagn. Invest."},{"issue":"4","key":"91926_CR28","doi-asserted-by":"publisher","first-page":"e00111-17","DOI":"10.1128\/CMR.00111-17","volume":"31","author":"N Kumar","year":"2018","unstructured":"Kumar, N., Sharma, S., Barua, S., Tripathi, B. N. & Rouse, B. T. Virological and immunological outcomes of coinfections. Clin. Microbiol. Rev. 31(4):e00111-17 https:\/\/doi.org\/10.1128\/CMR.00111-17 (2018).","journal-title":"Clin. Microbiol. Rev."},{"key":"91926_CR29","doi-asserted-by":"publisher","first-page":"12106","DOI":"10.1128\/JVI.79.18.12106-12111.2005","volume":"79","author":"T Ikegami","year":"2005","unstructured":"Ikegami, T., Won, S., Peters, C. J. & Makino, S. Rift Valley fever virus NSs mRNA is transcribed from an incoming anti-viral-sense S RNA segment. J. Virol. 79, 12106\u201312111 (2005).","journal-title":"J. Virol."},{"key":"91926_CR30","doi-asserted-by":"publisher","first-page":"839","DOI":"10.1111\/j.1600-0854.2005.00324.x","volume":"6","author":"B Schramm","year":"2005","unstructured":"Schramm, B. & Locker, J. K. Cytoplasmic organization of Poxvirus DNA replication. Traffic 6, 839\u2013846 (2005).","journal-title":"Traffic"},{"key":"91926_CR31","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1006\/viro.1997.8492","volume":"231","author":"IR Singh","year":"1997","unstructured":"Singh, I. R., Suomalainen, M., Varadarajan, S., Garoff, H. & Helenius, A. Multiple mechanisms for the inhibition of entry and uncoating of superinfecting Semliki Forest virus. Virology 231, 59\u201371 (1997).","journal-title":"Virology"},{"issue":"5","key":"91926_CR32","doi-asserted-by":"publisher","first-page":"e0156110","DOI":"10.1371\/journal.pone.0156110","volume":"11","author":"N Kumar","year":"2016","unstructured":"Kumar, N., Barua, S., Riyesh, T., Chaubey, K. K., Rawat, K. D., Khandelwal. N., et al. Complexities in isolation and purification of multiple viruses from mixed viral infections: viral interference, persistence and exclusion. PLoS ONE 11(5), e0156110. https:\/\/doi.org\/10.1371\/journal.pone.0156110 (2016).","journal-title":"PLoS ONE"},{"key":"91926_CR33","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/0042-6822(90)90051-R","volume":"174","author":"L Christen","year":"1990","unstructured":"Christen, L., Seto, J. & Niles, E. G. Superinfection exclusion of vaccinia virus in virus-infected cell cultures. Virology 174, 35\u201342 (1990).","journal-title":"Virology"},{"key":"91926_CR34","first-page":"161","volume":"114","author":"RP Kitching","year":"2003","unstructured":"Kitching, R. P. Vaccines for lumpy skin disease, sheep pox and goat pox. Dev. Biol. (Basel) 114, 161\u2013167 (2003).","journal-title":"Dev. Biol. (Basel)"},{"key":"91926_CR35","first-page":"165","volume":"10","author":"MS Abousenna","year":"2020","unstructured":"Abousenna, M. S., Sayed, R. H., Darwish, D. M. & Saad, M. A. Sensitivity of lateral flow technique for evaluation of inactivated Rift Valley fever virus vaccine in comparison with serum neutralization test. World Vet. J. 10, 165\u2013169 (2020).","journal-title":"World Vet. J."},{"key":"91926_CR36","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1136\/bmj.1.5123.663","volume":"1","author":"AB Sabin","year":"1959","unstructured":"Sabin, A. B. Present position of immunization against poliomyelitis with live virus vaccines. Br. Med. J. 1, 663\u2013680 (1959).","journal-title":"Br. Med. J."},{"key":"91926_CR37","doi-asserted-by":"publisher","first-page":"251","DOI":"10.3181\/00379727-128-32989","volume":"128","author":"EF Wheelock","year":"1968","unstructured":"Wheelock, E. F., Schenker, S. & Combes, B. Absence of circulating interferon in patients with infectious and serum Hepatitis. Exp. Biol. Med. 128, 251\u2013253 (1968).","journal-title":"Exp. Biol. Med."},{"key":"91926_CR38","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1007\/BF01310936","volume":"108","author":"X Li","year":"1989","unstructured":"Li, X. & Hanson, R. P. In vivo interference by Newcastle disease virus in chickens, the natural host of the virus. Arch. Virol. 108, 229\u2013245 (1989).","journal-title":"Arch. Virol."},{"issue":"2","key":"91926_CR39","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1128\/mr.51.2.179-191.1987","volume":"51","author":"P Whitaker-Dowling","year":"1987","unstructured":"Whitaker-Dowling, P. & Youngner, J. S. Viral interference-dominance of mutant viruses over wild-type virus in mixed infections. Microbiol. Rev. 51(2),  179\u2013191. https:\/\/doi.org\/10.1128\/mr.51.2.179-191.1987 (1987).","journal-title":"Microbiol. Rev."},{"key":"91926_CR40","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1637\/7930-020807-REGR.1","volume":"51","author":"J Gelb","year":"2007","unstructured":"Gelb, J., Ladman, B. S., Licata, M. J., Shapiro, M. H. & Campion, L. R. Evaluating viral interference between infectious bronchitis virus and newcastle disease virus vaccine strains using quantitative reverse transcription-polymerase chain reaction. Avian Dis. 51, 924\u2013934 (2007).","journal-title":"Avian Dis."},{"key":"91926_CR41","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1128\/br.24.1.151-166.1960","volume":"24","author":"RR Wagner","year":"1960","unstructured":"Wagner, R. R. Viral interference: Some considerations of basic mechanisms and their potential relationship to host resistance. Bacteriol. Rev. 24, 151 (1960).","journal-title":"Bacteriol. Rev."},{"key":"91926_CR42","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1186\/1743-422X-7-257","volume":"7","author":"KAS Thompson","year":"2010","unstructured":"Thompson, K. A. S. & Yin, J. Population dynamics of an RNA virus and its defective interfering particles in passage cultures. Virol. J. 7, 257 (2010).","journal-title":"Virol. J."},{"key":"91926_CR43","doi-asserted-by":"publisher","first-page":"72288","DOI":"10.1371\/journal.pone.0072288","volume":"8","author":"T Frensing","year":"2013","unstructured":"Frensing, T. et al. Continuous Influenza virus production in cell culture shows a periodic accumulation of defective interfering particles. PLoS ONE 8, 72288 (2013).","journal-title":"PLoS ONE"},{"key":"91926_CR44","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/rmv.641","volume":"20","author":"AC Marriott","year":"2010","unstructured":"Marriott, A. C. & Dimmock, N. J. Defective interfering viruses and their potential as antiviral agents. Rev. Med. Virol. 20, 51\u201362 (2010).","journal-title":"Rev. Med. Virol."},{"key":"91926_CR45","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/S0065-3527(08)60279-1","volume":"40","author":"L Roux","year":"1991","unstructured":"Roux, L., Simon, A. E. & Holland, J. J. Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Adv. Virus Res. 40, 181\u2013211 (1991).","journal-title":"Adv. Virus Res."},{"key":"91926_CR46","doi-asserted-by":"publisher","first-page":"3053","DOI":"10.1099\/0022-1317-77-12-3053","volume":"77","author":"A Billecocq","year":"1996","unstructured":"Billecocq, A., Vialat, P. & Bouloy, M. Persistent infection of mammalian cells by Rift Valley fever virus. J. Gen. Virol. 77, 3053\u20133062 (1996).","journal-title":"J. Gen. Virol."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-91926-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-91926-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-91926-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T09:53:16Z","timestamp":1670061196000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-91926-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,11]]},"references-count":46,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["91926"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-91926-5","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,11]]},"assertion":[{"value":"31 May 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 June 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"12395"}}