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Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15<jats:sub>(1\u201325)<\/jats:sub>, VP15<jats:sub>(26\u201357)<\/jats:sub>, VP15<jats:sub>(58\u201380)<\/jats:sub>, and VP15<jats:sub>(1\u201325,58\u201380)<\/jats:sub>. The purified proteins from the<jats:italic>E. coli<\/jats:italic>expression system were assayed as potential protective agents in Kuruma shrimp (<jats:italic>Marsupenaeus japonicus<\/jats:italic>) using the prime-and-boost strategy. Among the four truncated constructs, VP15<jats:sub>(26\u201357)<\/jats:sub>provided a significant improvement in the shrimp survival rate after 20\u00a0days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15<jats:sub>(26\u201357)<\/jats:sub>were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host<jats:italic>M. japonicus<\/jats:italic>were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15<jats:sub>(26\u201357)<\/jats:sub>, and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.<\/jats:p>","DOI":"10.1038\/s41598-021-92002-8","type":"journal-article","created":{"date-parts":[[2021,6,17]],"date-time":"2021-06-17T10:03:09Z","timestamp":1623924189000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Identification of antigenic domains and peptides from VP15 of white spot syndrome virus and their antiviral effects in Marsupenaeus japonicus"],"prefix":"10.1038","volume":"11","author":[{"given":"Jirayu","family":"Boonyakida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takafumi","family":"Nakanishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Mekata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Kato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enoch Y.","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,17]]},"reference":[{"key":"92002_CR1","doi-asserted-by":"publisher","first-page":"e13400","DOI":"10.1371\/journal.pone.0013400","volume":"5","author":"MP Zwart","year":"2010","unstructured":"Zwart, M. 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