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Normal maize is deficient in essential amino acids, lysine and tryptophan; and vitamin-A. Crop biofortification is a sustainable and economical approach to alleviate micronutrient malnutrition. We combined favorable alleles of <jats:italic>crtRB1<\/jats:italic> and <jats:italic>lcyE<\/jats:italic> genes into <jats:italic>opaque2<\/jats:italic> (<jats:italic>o2<\/jats:italic>)-based four inbreds viz<jats:italic>.<\/jats:italic> QLM11, QLM12, QLM13, and QLM14 using marker-assisted backcross breeding. These are parents of quality protein maize versions of two elite hybrids viz<jats:italic>.<\/jats:italic> Buland and PMH1, grown in India. Gene-based SSRs for <jats:italic>o2<\/jats:italic> and InDel markers for <jats:italic>crtRB1<\/jats:italic> and <jats:italic>lcyE<\/jats:italic> were successfully employed for foreground selection in BC<jats:sub>1<\/jats:sub>F<jats:sub>1<\/jats:sub>, BC<jats:sub>2<\/jats:sub>F<jats:sub>1<\/jats:sub>, and BC<jats:sub>2<\/jats:sub>F<jats:sub>2<\/jats:sub> generations. The recurrent parent genome recovery ranged from 88.9 to 96.0% among introgressed progenies. Kernels of pyramided lines possessed a high concentration of proA (7.14\u20139.63\u00a0ppm), compared to 1.05 to 1.41\u00a0ppm in the recurrent parents, while lysine and tryptophan ranged from 0.28\u20130.44% and 0.07\u20130.09%, respectively. The reconstituted hybrids (RBuland and RPMH1) showed significant enhancement of endosperm proA (6.97\u20139.82\u00a0ppm), tryptophan (0.07\u20130.09%), and lysine (0.29\u20130.43%), while grain yield was at par with their original versions. The dissemination of reconstituted hybrids holds significant promise to alleviate vitamin-A deficiency and protein-energy malnutrition in developing countries.<\/jats:p>","DOI":"10.1038\/s41598-021-92010-8","type":"journal-article","created":{"date-parts":[[2021,6,16]],"date-time":"2021-06-16T10:03:04Z","timestamp":1623837784000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Marker-assisted pyramiding of lycopene-\u03b5-cyclase, \u03b2-carotene hydroxylase1 and opaque2 genes for development of biofortified maize hybrids"],"prefix":"10.1038","volume":"11","author":[{"given":"Jagveer","family":"Singh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandeep","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5821-9345","authenticated-orcid":false,"given":"Yogesh","family":"Vikal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandeep Kaur","family":"Cheema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Balraj Kaur","family":"Bains","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorpreet","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gurjit Kaur","family":"Gill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawan Kumar","family":"Malhotra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priti","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vignesh","family":"Muthusamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amarjeet","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasbir Singh","family":"Chawla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firoz","family":"Hossain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,16]]},"reference":[{"issue":"6","key":"92010_CR1","first-page":"169","volume":"4","author":"LK Murdia","year":"2016","unstructured":"Murdia, L. 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