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The current study aimed to design a theranostic platform based on superparamagnetic iron oxide nanoparticles-pyoverdine (SPION\/PVD) conjugates bound to MUC1 aptamer (MUC1<jats:sub>Apt<\/jats:sub>) and loaded with doxorubicin (DOX) as an anti-cancer agent. The SPION\/PVD complex was covalently conjugated to MUC1<jats:sub>Apt<\/jats:sub> and loaded with DOX to prepare a targeted drug delivery system (SPION\/PVD\/MUC1<jats:sub>Apt<\/jats:sub>\/DOX). The investigation of cellular cytotoxicity and uptake of formulations by MTT and flow cytometry in both MUC1 positive (C26) and MUC1 negative (CHO) cell lines revealed that MUC1<jats:sub>Apt<\/jats:sub> could improve both cellular uptake and toxicity in the C26 cell line. The evaluation of tumor-targeting activity by in vivo bio-distribution showed that the targeted formulation could enhance tumor inhibitory growth effect and survival rate in C26 tumor-bearing mice. Furthermore, the potential of synthesized SPION\/PVD\/MUC1<jats:sub>Apt<\/jats:sub>\/DOX complex as diagnostic agents was investigated by magnetic resonance imaging (MRI) which improved the contrast of tumor site in MRI. Our findings confirm that aptamer-targeted PVD chelated the SPION as a diagnostic agent and loaded with DOX as a chemotherapeutic drug, would be beneficial as a novel theranostic platform.<\/jats:p>","DOI":"10.1038\/s41598-021-92391-w","type":"journal-article","created":{"date-parts":[[2021,6,22]],"date-time":"2021-06-22T10:02:49Z","timestamp":1624356169000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Targeted SPION siderophore conjugate loaded with doxorubicin as a theranostic agent for imaging and treatment of colon carcinoma"],"prefix":"10.1038","volume":"11","author":[{"given":"Rahim","family":"Nosrati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalil","family":"Abnous","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Alibolandi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jafar","family":"Mosafer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadegh","family":"Dehghani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad","family":"Taghdisi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Ramezani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,22]]},"reference":[{"key":"92391_CR1","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/j.tips.2014.06.007","volume":"35","author":"A G\u00f3rska","year":"2014","unstructured":"G\u00f3rska, A., Sloderbach, A. & Marsza\u0142\u0142, M. 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