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Here, however, we report that cyanogenic glycoside defenses from cassava (<jats:italic>Manihot esculenta<\/jats:italic>), a major staple crop in Africa, are activated during feeding by a pest insect, the whitefly <jats:italic>Bemisia tabaci<\/jats:italic>, and the resulting hydrogen cyanide is detoxified by conversion to beta-cyanoalanine. Additionally, <jats:italic>B. tabaci<\/jats:italic> was found to utilize two metabolic mechanisms to detoxify cyanogenic glucosides by conversion to non-activatable derivatives. First, the cyanogenic glycoside linamarin was glucosylated 1\u20134 times in succession in a reaction catalyzed by two <jats:italic>B. tabaci<\/jats:italic> glycoside hydrolase family 13 enzymes in vitro utilizing sucrose as a co-substrate. Second, both linamarin and the glucosylated linamarin derivatives were phosphorylated. Both phosphorylation and glucosidation of linamarin render this plant pro-toxin inert to the activating plant enzyme linamarase, and thus these metabolic transformations can be considered pre-emptive detoxification strategies to avoid cyanogenesis.<\/jats:p>","DOI":"10.1038\/s41598-021-92553-w","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T10:02:56Z","timestamp":1624528976000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Activation and detoxification of cassava cyanogenic glucosides by the whitefly Bemisia tabaci"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9601-8410","authenticated-orcid":false,"given":"Michael L. A. 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