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Two IM-C units were placed upstream and downstream of the DEP-D unit for enumeration, respectively, before and after the cells were treated in DEP-D unit, where the difference in cell count gave the total number of trapped cells based on their DEP characteristics. Conductivity of the running buffer was matched the conductivity of cytoplasm of wild type K562 and CCRF-CEM cells. Results showed that DEP responses of drug resistant and wild type K562 cells were statistically discriminative (at p\u2009=\u20090.05 level) at 200 mS\/m buffer conductivity and at 8.6\u00a0MHz working frequency of DEP-D unit. For CCRF-CEM cells, conductivity and frequency values were 160 mS\/m and 6.2\u00a0MHz, respectively. Our approach enabled discrimination of resistant cells in a group by setting up a threshold provided by the conductivity of running buffer. 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