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In this study, we generated inactivated virus-laden aerosols of 0.3\u20132 microns in diameter (0.9\u00a0\u00b5m mean diameter by mass) and dispersed the aerosolized viral particles onto electrostatic face mask filters. The limit of detection for inactivated coronaviruses SARS-CoV-2 and HCoV-NL63 extracted from filters was between 10 to 100\u00a0copies\/filter for both viruses. Testing for SARS-CoV-2, using face mask filters and nasopharyngeal swabs collected from hospitalized COVID-19-patients, showed that filter samples offered reduced sensitivity (8.5% compared to nasopharyngeal swabs). The low concordance of SARS-CoV-2 detection between filters and nasopharyngeal swabs indicated that number of viral particles collected on the face mask filter was below the limit of detection for all patients but those with the highest viral loads. This indicated face masks are unsuitable to replace diagnostic nasopharyngeal swabs in COVID-19 diagnosis. 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The commercial management of OML played no role in the design of the study, choice of enrolled patients, review and interpretation of data, preparation of the manuscript, or final approval of the manuscript. MPB, MC, and AEB are employed by inBiome B.V. SJM received no funds from OML. SJM is supported by the Medical Research Council (MR\/R009120\/1, MR\/V028669\/1), Engineering and Physical Sciences Research Council (EP\/R03558X\/1), British Lung Foundation (MKMRFPG18-6), June Hancock Mesothelioma Research Fund (JH-18-07), the NIHR Cambridge Biomedical Research Centre (BRC-1215-20014), Royal Papworth Hospital, and the Frank McCaughan is supported by the NIHR Cambridge Biomedical Research Centre (BRC). This paper represents independent research supported by the NIHR Cambridge Biomedical Research Centre (BRC-1215-20014). The views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care. The NIHR Cambridge Biomedical Research Centre (BRC) is a partnership between Cambridge University Hospitals NHS Foundation Trust and the University of Cambridge, funded by the National Institute for Health Research (NIHR). MM, TC, TD, CH, JG, PB, ND, VJ, PHMS, EVHB, WHVN, RL, MD, DC, BE, SP, AP, JG, ST declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"13476"}}