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The Bambara groundnut, an African underutilized legume, has the capacity to boost food and nutrition security while simultaneously addressing environmental sustainability, food availability, and economic inequalities. A set of 32 ISSRs were screened out of 96 primers based on very sharp, clear, and reproducible bands which detected a total of 510 loci with an average of 97.64% polymorphism. The average calculated value of\n                    <jats:italic>PIC<\/jats:italic>\n                    \u2009=\u20090.243,\n                    <jats:italic>RP<\/jats:italic>\n                    \u2009=\u20095.30,\n                    <jats:italic>H<\/jats:italic>\n                    \u2009=\u20090.285, and\n                    <jats:italic>MI<\/jats:italic>\n                    \u2009=\u20090.675 representing the efficiency of primer set for genetic differentiation among the genotypes. The ISSR primers revealed the number of alleles (\n                    <jats:italic>Na<\/jats:italic>\n                    \u2009=\u20091.97), the effective number of alleles (\n                    <jats:italic>Ne<\/jats:italic>\n                    \u2009=\u20091.38), Nei's genetic diversity (\n                    <jats:italic>h<\/jats:italic>\n                    \u2009=\u20090.248), and a moderate level of gene flow (\n                    <jats:italic>Nm<\/jats:italic>\n                    \u2009=\u20092.26) across the genotypes studied. The estimated Shannon\u2019s information index (\n                    <jats:italic>I<\/jats:italic>\n                    \u2009=\u20090.395) indicates a high level of genetic variation exists among the accessions. Based on Nei\u2019s genetic dissimilarity a UPMGA phylogenetic tree was constructed and grouped the entire genotypes into 3 major clusters and 6 subclusters. PCA analysis revealed that first principal component extracted maximum variation (PC1\u2009=\u200913.92%) than second principal component (PC2\u2009=\u200912.59%). Bayesian model-based STRUCTURE analysis assembled the genotypes into 3 (best \u0394K\u2009=\u20093) genetic groups. The fixation-index (F\n                    <jats:italic>st<\/jats:italic>\n                    ) analysis narrated a very great genetic diversity (F\n                    <jats:italic>st<\/jats:italic>\n                    \u2009=\u20090.19 to 0.40) exists within the accessions of these 3 clusters. This investigation specifies the effectiveness of the ISSR primers system for the molecular portrayal of\n                    <jats:italic>V. subterranea<\/jats:italic>\n                    genotypes that could be used for genetic diversity valuation, detection, and tagging of potential genotypes with quick, precise, and authentic measures for this crop improvement through effective breeding schemes.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-93867-5","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T06:03:50Z","timestamp":1626329030000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["DNA fingerprinting, fixation-index (Fst), and admixture mapping of selected Bambara groundnut (Vigna subterranea [L.] Verdc.) accessions using ISSR markers system"],"prefix":"10.1038","volume":"11","author":[{"given":"Md Mahmudul Hasan","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd Y.","family":"Rafii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shairul Izan","family":"Ramlee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mashitah","family":"Jusoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md","family":"Al Mamun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamilu","family":"Halidu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,15]]},"reference":[{"key":"93867_CR1","unstructured":"Rungnoi, O., Suwanprasert, J., Somta, P., & Srinives, P. 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