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Salinization of coastal freshwater habitats is a growing concern in a changing climate due to increased frequency and intensity of storm surges and drought conditions. This study opportunistically sampled juvenile male and female wild alligators in various salinities each month excluding November, December, and January for one year at Rockefeller Wildlife Refuge in coastal Louisiana. Blood plasma biochemistry parameters including electrolyte levels were subsequently measured. In addition, levels of various renin\u2013angiotensin\u2013aldosterone system hormones, glucocorticoids, androgens, estrogens, and progestogens were analyzed using liquid chromatography and tandem mass spectrometry. Only males were sampled in hyperosmotic environments (&gt;\u200910\u2030) during dry conditions in late summer 2018. In juvenile males, plasma Na<jats:sup>+<\/jats:sup>, Cl<jats:sup>\u2212<\/jats:sup>, and the progestogen 17\u03b1,20\u03b2-dihydroxypregnenone were significantly and positively correlated with environmental salinity. However, variation in glucocorticoids, androgens, and estrogens were not associated with hypersaline water while sex steroids showed significant seasonal variation. This study demonstrated significant correlation of environmental salinity with electrolyte levels and a sex steroid in wild juvenile alligators, and to our knowledge represents the first measurement of 17\u03b1,20\u03b2-dihydroxypregnenone in alligators.<\/jats:p>","DOI":"10.1038\/s41598-021-94557-y","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T10:10:53Z","timestamp":1627294253000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Correlations between environmental salinity levels, blood biochemistry parameters, and steroid hormones in wild juvenile American alligators (Alligator mississippiensis)"],"prefix":"10.1038","volume":"11","author":[{"given":"Patricia C.","family":"Faulkner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth M.","family":"Elsey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Hala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lene H.","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,7,26]]},"reference":[{"key":"94557_CR1","volume-title":"Histoire naturelle, g\u00e9n\u00e9rale et particuli\u00e8re des reptiles: ouvrage faisant suite \u00e0 l'histoire naturelle g\u00e9n\u00e9rale et particulie\u00e8re, compos\u00e9e par Leclerc de Buffon, et r\u00e9dig\u00e9e par CS Sonnini, membre de plusieurs soci\u00e9t\u00e9s savantes","author":"FM Daudin","year":"1802","unstructured":"Daudin, F. 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