{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T02:49:54Z","timestamp":1793674194179,"version":"4.5.1"},"reference-count":40,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]},{"name":"NIH","award":["P01HL101871"],"award-info":[{"award-number":["P01HL101871"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Endothelial hemoglobin (Hb)\u03b1 regulates endothelial nitric oxide synthase (eNOS) biochemistry. We hypothesized that Hb could also be expressed and biochemically active in the ciliated human airway epithelium. Primary human airway epithelial cells, cultured at air\u2013liquid interface (ALI), were obtained by clinical airway brushings or from explanted lungs. Human airway Hb mRNA data were from publically available databases; or from RT-PCR. Hb proteins were identified by immunoprecipitation, immunoblot, immunohistochemistry, immunofluorescence and liquid chromatography- mass spectrometry. Viral vectors were used to alter Hb\u03b2 expression. Heme and nitrogen oxides were measured colorimetrically. Hb mRNA was expressed in human ciliated epithelial cells. Heme proteins (Hb\u03b1, \u03b2, and \u03b4) were detected in ALI cultures by several methods. Higher levels of airway epithelial Hb\u03b2 gene expression were associated with lower FEV<jats:sub>1<\/jats:sub> in asthma. Both Hb\u03b2 knockdown and overexpression affected cell morphology. Hb\u03b2 and eNOS were apically colocalized. Binding heme with CO decreased extracellular accumulation of nitrogen oxides. Human airway epithelial cells express Hb. Higher levels of Hb\u03b2 gene expression were associated with airflow obstruction. Hb\u03b2 and eNOS were colocalized in ciliated cells, and heme affected oxidation of the NOS product. Epithelial Hb expression may be relevant to human airways diseases.<\/jats:p>","DOI":"10.1038\/s41598-021-94782-5","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T10:03:26Z","timestamp":1627553006000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Somatic cell hemoglobin modulates nitrogen oxide metabolism in the human airway epithelium"],"prefix":"10.1038","volume":"11","author":[{"given":"Nadzeya","family":"Marozkina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Zein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James F.","family":"Chmiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeeho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Kiselar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah S.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott H.","family":"Randell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Gaston","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,29]]},"reference":[{"key":"94782_CR1","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1038\/nature11626","volume":"491","author":"AC Straub","year":"2012","unstructured":"Straub, A. C. et al. Endothelial cell expression of haemoglobin alpha regulates nitric oxide signalling. Nature 491, 473\u2013477. https:\/\/doi.org\/10.1038\/nature11626 (2012).","journal-title":"Nature"},{"key":"94782_CR2","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1177\/002215540305100110","volume":"51","author":"X Zhan","year":"2003","unstructured":"Zhan, X., Li, D. & Johns, R. A. Expression of endothelial nitric oxide synthase in ciliated epithelia of rats. J. Histochem. Cytochem. 51, 81\u201387. https:\/\/doi.org\/10.1177\/002215540305100110 (2003).","journal-title":"J. Histochem. Cytochem."},{"key":"94782_CR3","doi-asserted-by":"publisher","first-page":"L247","DOI":"10.1152\/ajplung.00020.2019","volume":"317","author":"N Marozkina","year":"2019","unstructured":"Marozkina, N. et al. Cyclic compression increases F508 Del CFTR expression in ciliated human airway epithelium. Am. J. Physiol. Lung Cell. Mol. Physiol. 317, L247\u2013L258. https:\/\/doi.org\/10.1152\/ajplung.00020.2019 (2019).","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"94782_CR4","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1146\/annurev-physiol-021113-170352","volume":"77","author":"NV Marozkina","year":"2015","unstructured":"Marozkina, N. V. & Gaston, B. Nitrogen chemistry and lung physiology. Annu. Rev. Physiol. 77, 431\u2013452. https:\/\/doi.org\/10.1146\/annurev-physiol-021113-170352 (2015).","journal-title":"Annu. Rev. Physiol."},{"key":"94782_CR5","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.1084\/jem.189.10.1621","volume":"189","author":"GT De Sanctis","year":"1999","unstructured":"De Sanctis, G. T. et al. Contribution of nitric oxide synthases 1, 2, and 3 to airway hyperresponsiveness and inflammation in a murine model of asthma. J. Exp. Med. 189, 1621\u20131630 (1999).","journal-title":"J. Exp. Med."},{"key":"94782_CR6","doi-asserted-by":"publisher","first-page":"S34","DOI":"10.1016\/j.jaci.2011.12.983","volume":"129","author":"A Fuhlbrigge","year":"2012","unstructured":"Fuhlbrigge, A. et al. Asthma outcomes: Exacerbations. J. Allergy Clin. Immunol. 129, S34-48. https:\/\/doi.org\/10.1016\/j.jaci.2011.12.983 (2012).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94782_CR7","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1016\/j.jpeds.2011.08.024","volume":"160","author":"S Sundararajan","year":"2012","unstructured":"Sundararajan, S. & Gaston, B. Sickle cell disease does not decrease pulmonary nitric oxide. J. Pediatr. 160, 6\u20137. https:\/\/doi.org\/10.1016\/j.jpeds.2011.08.024 (2012).","journal-title":"J. Pediatr."},{"key":"94782_CR8","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1007\/s10875-019-00613-8","volume":"39","author":"ZN Zysman-Colman","year":"2019","unstructured":"Zysman-Colman, Z. N. et al. Nasal nitric oxide in primary immunodeficiency and primary ciliary dyskinesia: Helping to distinguish between clinically similar diseases. J. Clin. Immunol. 39, 216\u2013224. https:\/\/doi.org\/10.1007\/s10875-019-00613-8 (2019).","journal-title":"J. Clin. Immunol."},{"key":"94782_CR9","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1007\/978-1-62703-125-7_8","volume":"945","author":"ML Fulcher","year":"2013","unstructured":"Fulcher, M. L. & Randell, S. H. Human nasal and tracheo-bronchial respiratory epithelial cell culture. Methods Mol. Biol. 945, 109\u2013121. https:\/\/doi.org\/10.1007\/978-1-62703-125-7_8 (2013).","journal-title":"Methods Mol. Biol."},{"key":"94782_CR10","doi-asserted-by":"publisher","first-page":"C468","DOI":"10.1152\/ajpcell.1998.275.2.C468","volume":"275","author":"WJ Perkins","year":"1998","unstructured":"Perkins, W. J., Pabelick, C., Warner, D. O. & Jones, K. A. cGMP-independent mechanism of airway smooth muscle relaxation induced by S-nitrosoglutathione. Am. J. Physiol. 275, C468-474 (1998).","journal-title":"Am. J. Physiol."},{"key":"94782_CR11","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1016\/j.taap.2017.06.010","volume":"331","author":"BC Goodale","year":"2017","unstructured":"Goodale, B. C., Rayack, E. J. & Stanton, B. A. Arsenic alters transcriptional responses to Pseudomonas aeruginosa infection and decreases antimicrobial defense of human airway epithelial cells. Toxicol. Appl. Pharmacol. 331, 154\u2013163. https:\/\/doi.org\/10.1016\/j.taap.2017.06.010 (2017).","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"94782_CR12","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1038\/s41586-018-0394-6","volume":"560","author":"LW Plasschaert","year":"2018","unstructured":"Plasschaert, L. W. et al. A single-cell atlas of the airway epithelium reveals the CFTR-rich pulmonary ionocyte. Nature 560, 377\u2013381. https:\/\/doi.org\/10.1038\/s41586-018-0394-6 (2018).","journal-title":"Nature"},{"key":"94782_CR13","doi-asserted-by":"publisher","first-page":"2856","DOI":"10.1038\/nprot.2006.468","volume":"1","author":"A Shevchenko","year":"2006","unstructured":"Shevchenko, A., Tomas, H., Havlis, J., Olsen, J. V. & Mann, M. In-gel digestion for mass spectrometric characterization of proteins and proteomes. Nat. Protoc. 1, 2856\u20132860. https:\/\/doi.org\/10.1038\/nprot.2006.468 (2006).","journal-title":"Nat. Protoc."},{"key":"94782_CR14","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.jchromb.2007.01.052","volume":"851","author":"A Gow","year":"2007","unstructured":"Gow, A., Doctor, A., Mannick, J. & Gaston, B. S-Nitrosothiol measurements in biological systems. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 851, 140\u2013151. https:\/\/doi.org\/10.1016\/j.jchromb.2007.01.052 (2007).","journal-title":"J. Chromatogr. B Anal. Technol. Biomed. Life Sci."},{"key":"94782_CR15","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/S0021-9258(18)74965-X","volume":"112","author":"DL Drabkin","year":"1935","unstructured":"Drabkin, D. L. & Austin, J. H. Spectrophotometric studies: II. Preparations from washed blood cells; nitric oxide hemoglobin and sulfhemoglobin. J. Biol. Chem. 112, 51\u201365. https:\/\/doi.org\/10.1016\/S0021-9258(18)74965-X (1935).","journal-title":"J. Biol. Chem."},{"key":"94782_CR16","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1016\/j.jaci.2011.02.042","volume":"128","author":"WC Moore","year":"2011","unstructured":"Moore, W. C. et al. Safety of investigative bronchoscopy in the Severe Asthma Research Program. J. Allergy Clin. Immunol. 128, 328-336.e323. https:\/\/doi.org\/10.1016\/j.jaci.2011.02.042 (2011).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94782_CR17","doi-asserted-by":"publisher","first-page":"1363","DOI":"10.1164\/rccm.201406-1099OC","volume":"190","author":"BD Modena","year":"2014","unstructured":"Modena, B. D. et al. Gene expression in relation to exhaled nitric oxide identifies novel asthma phenotypes with unique biomolecular pathways. Am. J. Respir. Crit. Care Med. 190, 1363\u20131372. https:\/\/doi.org\/10.1164\/rccm.201406-1099OC (2014).","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"94782_CR18","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1164\/rccm.200906-0896OC","volume":"181","author":"WC Moore","year":"2010","unstructured":"Moore, W. C. et al. Identification of asthma phenotypes using cluster analysis in the Severe Asthma Research Program. Am. J. Respir. Crit. Care Med. 181, 315\u2013323. https:\/\/doi.org\/10.1164\/rccm.200906-0896OC (2010).","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"94782_CR19","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1093\/nar\/30.1.207","volume":"30","author":"R Edgar","year":"2002","unstructured":"Edgar, R., Domrachev, M. & Lash, A. E. Gene expression omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 30, 207\u2013210. https:\/\/doi.org\/10.1093\/nar\/30.1.207 (2002).","journal-title":"Nucleic Acids Res."},{"key":"94782_CR20","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/j.jaci.2006.11.639","volume":"119","author":"WC Moore","year":"2007","unstructured":"Moore, W. C. et al. Characterization of the severe asthma phenotype by the national heart, lung, and blood institute\u2019s severe asthma research program. J. Allergy Clin. Immunol. 119, 405\u2013413 (2007).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94782_CR21","doi-asserted-by":"publisher","first-page":"9853","DOI":"10.1111\/jcmm.15568","volume":"24","author":"JL Kerschner","year":"2020","unstructured":"Kerschner, J. L. et al. A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface. J. Cell Mol. Med. 24, 9853\u20139870. https:\/\/doi.org\/10.1111\/jcmm.15568 (2020).","journal-title":"J. Cell Mol. Med."},{"key":"94782_CR22","doi-asserted-by":"publisher","first-page":"10089","DOI":"10.1073\/pnas.91.21.10089","volume":"91","author":"K Asano","year":"1994","unstructured":"Asano, K. et al. Constitutive and inducible nitric oxide synthase gene expression, regulation, and activity in human lung epithelial cells. Proc. Natl. Acad. Sci. U S A 91, 10089\u201310093 (1994).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"94782_CR23","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1165\/ajrcmb\/9.4.371","volume":"9","author":"L Kobzik","year":"1993","unstructured":"Kobzik, L. et al. Nitric oxide synthase in human and rat lung: Immunocytochemical and histochemical localization. Am. J. Respir. Cell Mol. Biol. 9, 371\u2013377. https:\/\/doi.org\/10.1165\/ajrcmb\/9.4.371 (1993).","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"94782_CR24","doi-asserted-by":"publisher","first-page":"5668","DOI":"10.1074\/jbc.M509314200","volume":"281","author":"DA Newton","year":"2006","unstructured":"Newton, D. A., Rao, K. M., Dluhy, R. A. & Baatz, J. E. Hemoglobin is expressed by alveolar epithelial cells. J. Biol. Chem. 281, 5668\u20135676. https:\/\/doi.org\/10.1074\/jbc.M509314200 (2006).","journal-title":"J. Biol. Chem."},{"key":"94782_CR25","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.1016\/j.bbrc.2005.06.042","volume":"333","author":"M Bhaskaran","year":"2005","unstructured":"Bhaskaran, M., Chen, H., Chen, Z. & Liu, L. Hemoglobin is expressed in alveolar epithelial type II cells. Biochem. Biophys. Res. Commun. 333, 1348\u20131352. https:\/\/doi.org\/10.1016\/j.bbrc.2005.06.042 (2005).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"94782_CR26","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1186\/1465-9921-11-123","volume":"11","author":"N Ishikawa","year":"2010","unstructured":"Ishikawa, N. et al. Hemoglobin \u03b1 and \u03b2 are ubiquitous in the human lung, decline in idiopathic pulmonary fibrosis but not in COPD. Respir. Res. 11, 123. https:\/\/doi.org\/10.1186\/1465-9921-11-123 (2010).","journal-title":"Respir. Res."},{"key":"94782_CR27","doi-asserted-by":"publisher","first-page":"1911","DOI":"10.1167\/iovs.07-1372","volume":"50","author":"TH Tezel","year":"2009","unstructured":"Tezel, T. H. et al. Synthesis and secretion of hemoglobin by retinal pigment epithelium. Investig. Ophthalmol. Vis. Sci. 50, 1911\u20131919. https:\/\/doi.org\/10.1167\/iovs.07-1372 (2009).","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"94782_CR28","doi-asserted-by":"publisher","first-page":"1500","DOI":"10.1681\/asn.2007101085","volume":"19","author":"H Nishi","year":"2008","unstructured":"Nishi, H. et al. Hemoglobin is expressed by mesangial cells and reduces oxidant stress. J. Am. Soc. Nephrol. 19, 1500\u20131508. https:\/\/doi.org\/10.1681\/asn.2007101085 (2008).","journal-title":"J. Am. Soc. Nephrol."},{"key":"94782_CR29","doi-asserted-by":"publisher","first-page":"e24363","DOI":"10.1371\/journal.pone.0024363","volume":"6","author":"W Liu","year":"2011","unstructured":"Liu, W., Baker, S. S., Baker, R. D., Nowak, N. J. & Zhu, L. Upregulation of hemoglobin expression by oxidative stress in hepatocytes and its implication in nonalcoholic steatohepatitis. PLoS ONE 6, e24363. https:\/\/doi.org\/10.1371\/journal.pone.0024363 (2011).","journal-title":"PLoS ONE"},{"key":"94782_CR30","doi-asserted-by":"publisher","first-page":"15454","DOI":"10.1073\/pnas.0813216106","volume":"106","author":"M Biagioli","year":"2009","unstructured":"Biagioli, M. et al. Unexpected expression of alpha- and beta-globin in mesencephalic dopaminergic neurons and glial cells. Proc. Natl. Acad. Sci. U S A 106, 15454\u201315459. https:\/\/doi.org\/10.1073\/pnas.0813216106 (2009).","journal-title":"Proc. Natl. Acad. Sci. U S A"},{"key":"94782_CR31","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1093\/humrep\/dem430","volume":"23","author":"H Dassen","year":"2008","unstructured":"Dassen, H. et al. Haemoglobin expression in human endometrium. Hum. Reprod. 23, 635\u2013641. https:\/\/doi.org\/10.1093\/humrep\/dem430 (2008).","journal-title":"Hum. Reprod."},{"key":"94782_CR32","doi-asserted-by":"publisher","first-page":"e54342","DOI":"10.1371\/journal.pone.0054342","volume":"8","author":"X Li","year":"2013","unstructured":"Li, X. et al. Characterization of adult \u03b1- and \u03b2-globin elevated by hydrogen peroxide in cervical cancer cells that play a cytoprotective role against oxidative insults. PLoS ONE 8, e54342. https:\/\/doi.org\/10.1371\/journal.pone.0054342 (2013).","journal-title":"PLoS ONE"},{"key":"94782_CR33","doi-asserted-by":"publisher","first-page":"6643","DOI":"10.1073\/pnas.96.12.6643","volume":"96","author":"L Liu","year":"1999","unstructured":"Liu, L., Zeng, M. & Stamler, J. S. Hemoglobin induction in mouse macrophages. Proc. Natl. Acad. Sci. 96, 6643\u20136647. https:\/\/doi.org\/10.1073\/pnas.96.12.6643 (1999).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"94782_CR34","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.mce.2009.12.022","volume":"317","author":"L Mikkonen","year":"2010","unstructured":"Mikkonen, L., Pihlajamaa, P., Sahu, B., Zhang, F. P. & J\u00e4nne, O. A. Androgen receptor and androgen-dependent gene expression in lung. Mol. Cell Endocrinol. 317, 14\u201324. https:\/\/doi.org\/10.1016\/j.mce.2009.12.022 (2010).","journal-title":"Mol. Cell Endocrinol."},{"key":"94782_CR35","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1165\/rcmb.2009-0307OC","volume":"44","author":"CL Grek","year":"2011","unstructured":"Grek, C. L., Newton, D. A., Spyropoulos, D. D. & Baatz, J. E. Hypoxia up-regulates expression of hemoglobin in alveolar epithelial cells. Am. J. Respir. Cell Mol. Biol. 44, 439\u2013447. https:\/\/doi.org\/10.1165\/rcmb.2009-0307OC (2011).","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"94782_CR36","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/S0076-6879(08)36008-X","volume":"436","author":"M Angelo","year":"2008","unstructured":"Angelo, M., Hausladen, A., Singel, D. & Stamler, J. Interactions of NO with hemoglobin: From microbes to man. Methods Enzymol. 436, 131\u2013168. https:\/\/doi.org\/10.1016\/S0076-6879(08)36008-X (2008).","journal-title":"Methods Enzymol."},{"key":"94782_CR37","first-page":"978","volume":"268","author":"B Gaston","year":"1994","unstructured":"Gaston, B. et al. Relaxation of human bronchial smooth muscle by S-nitrosothiols in vitro. J. Pharmacol. Exp. Ther.. 268, 978\u2013984 (1994).","journal-title":"J. Pharmacol. Exp. Ther.."},{"key":"94782_CR38","doi-asserted-by":"publisher","first-page":"E1117","DOI":"10.1073\/pnas.1717993115","volume":"115","author":"A Ghosh","year":"2018","unstructured":"Ghosh, A., Garee, G., Sweeny, E. A., Nakamura, Y. & Stuehr, D. J. Hsp90 chaperones hemoglobin maturation in erythroid and nonerythroid cells. Proc. Natl. Acad. Sci. 115, E1117. https:\/\/doi.org\/10.1073\/pnas.1717993115 (2018).","journal-title":"Proc. Natl. Acad. Sci."},{"key":"94782_CR39","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1089\/ars.2009.2780","volume":"12","author":"TL Mollan","year":"2010","unstructured":"Mollan, T. L., Yu, X., Weiss, M. J. & Olson, J. S. The role of alpha-hemoglobin stabilizing protein in redox chemistry, denaturation, and hemoglobin assembly. Antioxid. Redox Signal. 12, 219\u2013231. https:\/\/doi.org\/10.1089\/ars.2009.2780 (2010).","journal-title":"Antioxid. Redox Signal."},{"key":"94782_CR40","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1183\/09031936.98.12051124","volume":"12","author":"F Santoli","year":"1998","unstructured":"Santoli, F. et al. Pulmonary function in sickle cell disease with or without acute chest syndrome. Eur. Respir. J. 12, 1124\u20131129. https:\/\/doi.org\/10.1183\/09031936.98.12051124 (1998).","journal-title":"Eur. Respir. J."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94782-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94782-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94782-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T11:47:09Z","timestamp":1670068029000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94782-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,29]]},"references-count":40,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["94782"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-94782-5","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,29]]},"assertion":[{"value":"27 January 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 July 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15498"}}