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Its effective control will require the accurate identification of infected individuals, which has been restricted to nucleic acid-based tests due to similarities with other emerging members of the Alphavirus genus of the Togaviridae family; both in structure and clinical symptoms. Serological tests have a more significant potential to expand testing at a reasonable cost, and their performance primarily reflects that of the antigen utilized to capture pathogen-specific antibodies. Here, we describe the assembly of a synthetic gene encoding multiple copies of antigenic determinants mapped from the nsP1, nsP2, E1, and E2 proteins of MAYV that readily expressed as a stable chimeric protein in bacteria. Its serological performance as the target in ELISAs revealed a high accuracy for detecting anti-MAYV IgM antibodies. No cross-reactivity was observed with serum from seropositive individuals for dengue, chikungunya, yellow fever, Zika, and other infectious diseases as well as healthy individuals. Our data suggest that this bioengineered antigen could be used to develop high-performance serological tests for MAYV infections.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-94817-x","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T06:02:43Z","timestamp":1627452163000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Performance assessment of a multi-epitope chimeric antigen for the serological diagnosis of acute Mayaro fever"],"prefix":"10.1038","volume":"11","author":[{"given":"Paloma","family":"Napole\u00e3o-P\u00eago","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via R. 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It should be stated that the peptides, proteins, and methodology described in this study are protected under Brazilian and U.S. provisional patent application BR 10.2019.017792.6 and PCT\/BR2020\/050341, respectively, filed and controlled by the non-profit Oswaldo Cruz Foundation (FIOCRUZ) and may serve as a future source of funding. The funding agencies had no role in study design, data collection, data analysis, decision to publish, or preparation of the manuscript.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15374"}}