{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:46:04Z","timestamp":1787921164362,"version":"build-2784847793"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T00:00:00Z","timestamp":1627516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000057","name":"NIGMS","doi-asserted-by":"crossref","award":["P20GM130555"],"award-info":[{"award-number":["P20GM130555"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Increased eosinophil recruitment is a hallmark feature of eosinophilic disorders. Here, we delineated the key molecular and cellular players involved in physiological eosinophilic recruitment during normal postnatal lung development in mice. Physiological eosinophilic recruitment was consistently present in 7-, 10-, and 15-day-old neonatal mice, but not in 42-day-old mice. This feature was completely abolished in interleukin 33 (IL-33)-, interleukin 2 receptor gamma chain (IL2r\u03b3)-, and interleukin 4 receptor alpha (IL4R\u03b1)-knockout mice, but not in recombination activating gene 1 (Rag1)-knockout mice demonstrating an indispensable role for IL-33, innate lymphoid cells (ILCs), and IL4R\u03b1 in eosinophil recruitment. Interestingly, myeloid-specific IL4R\u03b1-deficient (mye-IL4R\u03b1<jats:sup>\u2212\/\u2212<\/jats:sup>) mice had significantly reduced eosinophilia in the airspaces that was associated with reduced levels of IL-4 and IL-5 in the bronchoalveolar lavage fluid (BALF). Further, we tested the effect of myeloid-specific IL4R\u03b1 deficiency on IL-13-induced eosinophil recruitment into adult lung airspaces. Eosinophil recruitment into the airspaces was elevated in IL-13-treated WT mice but not in IL-13-treated mye-IL4R\u03b1<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Consistent with the degree of eosinophilia, the BALF levels of eosinophil recruitment-associated cytokines were significantly elevated in IL-13-treated WT but not in IL-13-treated mye-IL4R\u03b1<jats:sup>\u2212\/\u2212<\/jats:sup> mice. These data establish that myeloid-IL4R\u03b1 is an indispensable component of the IL-33-ILCsIL-13-IL4R\u03b1 axis of eosinophil recruitment.<\/jats:p>","DOI":"10.1038\/s41598-021-94843-9","type":"journal-article","created":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T10:03:26Z","timestamp":1627553006000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Myeloid-IL4R\u03b1 is an indispensable link in IL-33-ILCs-IL-13-IL4R\u03b1 axis of eosinophil recruitment in murine lungs"],"prefix":"10.1038","volume":"11","author":[{"given":"Sonika","family":"Patial","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon W.","family":"Lewis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thao","family":"Vo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ishita","family":"Choudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kshitiz","family":"Paudel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhruthi","family":"Singamsetty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Brombacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yogesh","family":"Saini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,29]]},"reference":[{"key":"94843_CR1","doi-asserted-by":"publisher","first-page":"3882","DOI":"10.1182\/blood-2012-06-330845","volume":"120","author":"EA Jacobsen","year":"2012","unstructured":"Jacobsen, E. A., Helmers, R. A., Lee, J. J. & Lee, N. A. The expanding role(s) of eosinophils in health and disease. Blood 120, 3882\u20133890. https:\/\/doi.org\/10.1182\/blood-2012-06-330845 (2012).","journal-title":"Blood"},{"key":"94843_CR2","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1007\/s11882-007-0026-y","volume":"7","author":"EA Jacobsen","year":"2007","unstructured":"Jacobsen, E. A., Ochkur, S. I., Lee, N. A. & Lee, J. J. Eosinophils and asthma. Curr. Allergy Asthma Rep. 7, 18\u201326 (2007).","journal-title":"Curr. Allergy Asthma Rep."},{"key":"94843_CR3","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/j.it.2004.07.006","volume":"25","author":"AB Kay","year":"2004","unstructured":"Kay, A. B., Phipps, S. & Robinson, D. S. A role for eosinophils in airway remodelling in asthma. Trends Immunol. 25, 477\u2013482. https:\/\/doi.org\/10.1016\/j.it.2004.07.006 (2004).","journal-title":"Trends Immunol."},{"key":"94843_CR4","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.jaip.2015.01.013","volume":"3","author":"F Legrand","year":"2015","unstructured":"Legrand, F. & Klion, A. D. Biologic therapies targeting eosinophils: Current status and future prospects. J. Allergy Clin. Immunol. 3, 167\u2013174. https:\/\/doi.org\/10.1016\/j.jaip.2015.01.013 (2015).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94843_CR5","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1038\/nrd3838","volume":"12","author":"PC Fulkerson","year":"2013","unstructured":"Fulkerson, P. C. & Rothenberg, M. E. Targeting eosinophils in allergy, inflammation and beyond. Nat. Rev. Drug Discov. 12, 117\u2013129. https:\/\/doi.org\/10.1038\/nrd3838 (2013).","journal-title":"Nat. Rev. Drug Discov."},{"key":"94843_CR6","doi-asserted-by":"publisher","first-page":"3879","DOI":"10.1038\/s41467-018-06316-9","volume":"9","author":"S Yi","year":"2018","unstructured":"Yi, S. et al. Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge. Nat. Commun. 9, 3879. https:\/\/doi.org\/10.1038\/s41467-018-06316-9 (2018).","journal-title":"Nat. Commun."},{"key":"94843_CR7","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1067\/mai.2001.118600","volume":"108","author":"SM Pope","year":"2001","unstructured":"Pope, S. M. et al. IL-13 induces eosinophil recruitment into the lung by an IL-5- and eotaxin-dependent mechanism. J. Allergy Clin. Immunol. 108, 594\u2013601. https:\/\/doi.org\/10.1067\/mai.2001.118600 (2001).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94843_CR8","doi-asserted-by":"publisher","first-page":"1037","DOI":"10.1189\/jlb.3RI1214-595R","volume":"97","author":"JM Lott","year":"2015","unstructured":"Lott, J. M., Sumpter, T. L. & Turnquist, H. R. New dog and new tricks: Evolving roles for IL-33 in type 2 immunity. J. Leukoc. Biol. 97, 1037\u20131048. https:\/\/doi.org\/10.1189\/jlb.3RI1214-595R (2015).","journal-title":"J. Leukoc. Biol."},{"key":"94843_CR9","doi-asserted-by":"publisher","first-page":"136","DOI":"10.3389\/fmed.2017.00136","volume":"4","author":"MC Larose","year":"2017","unstructured":"Larose, M. C., Archambault, A. S., Provost, V., Laviolette, M. & Flamand, N. Regulation of eosinophil and group 2 innate lymphoid cell trafficking in asthma. Front. Med. 4, 136. https:\/\/doi.org\/10.3389\/fmed.2017.00136 (2017).","journal-title":"Front. Med."},{"key":"94843_CR10","doi-asserted-by":"publisher","first-page":"1422","DOI":"10.1016\/j.jaci.2014.05.011","volume":"134","author":"D Li","year":"2014","unstructured":"Li, D. et al. IL-33 promotes ST2-dependent lung fibrosis by the induction of alternatively activated macrophages and innate lymphoid cells in mice. J. Allergy Clin. Immunol. 134, 1422\u20131432. https:\/\/doi.org\/10.1016\/j.jaci.2014.05.011 (2014).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94843_CR11","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.4049\/jimmunol.2000530","volume":"205","author":"BW Lewis","year":"2020","unstructured":"Lewis, B. W. et al. The innate lymphoid system is a critical player in the manifestation of mucoinflammatory airway disease in mice. J. Immunol. 205, 1695\u20131708. https:\/\/doi.org\/10.4049\/jimmunol.2000530 (2020).","journal-title":"J. Immunol."},{"key":"94843_CR12","doi-asserted-by":"publisher","first-page":"4357","DOI":"10.4049\/jimmunol.0802557","volume":"182","author":"A Livraghi","year":"2009","unstructured":"Livraghi, A. et al. Airway and lung pathology due to mucosal surface dehydration in beta}-epithelial Na+ channel-overexpressing mice: Role of TNF-{alpha and IL-4R{alpha} signaling, influence of neonatal development, and limited efficacy of glucocorticoid treatment. J. Immunol. 182, 4357\u20134367. https:\/\/doi.org\/10.4049\/jimmunol.0802557 (2009).","journal-title":"J. Immunol."},{"key":"94843_CR13","doi-asserted-by":"publisher","first-page":"18581","DOI":"10.1073\/pnas.1003059107","volume":"107","author":"K Oboki","year":"2010","unstructured":"Oboki, K. et al. IL-33 is a crucial amplifier of innate rather than acquired immunity. Proc. Natl. Acad. Sci. USA 107, 18581\u201318586. https:\/\/doi.org\/10.1073\/pnas.1003059107 (2010).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"94843_CR14","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/S1074-7613(04)00107-4","volume":"20","author":"DR Herbert","year":"2004","unstructured":"Herbert, D. R. et al. Alternative macrophage activation is essential for survival during schistosomiasis and downmodulates T helper 1 responses and immunopathology. Immunity 20, 623\u2013635 (2004).","journal-title":"Immunity"},{"key":"94843_CR15","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1165\/rcmb.2014-0111OC","volume":"54","author":"Y Saini","year":"2016","unstructured":"Saini, Y. et al. Neonatal pulmonary macrophage depletion coupled to defective mucus clearance increases susceptibility to pneumonia and alters pulmonary immune responses. Am. J. Respir. Cell Mol. Biol. 54, 210\u2013221. https:\/\/doi.org\/10.1165\/rcmb.2014-0111OC (2016).","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"94843_CR16","doi-asserted-by":"publisher","first-page":"1170","DOI":"10.4049\/jimmunol.1700144","volume":"199","author":"BW Lewis","year":"2017","unstructured":"Lewis, B. W. et al. Early postnatal secondhand smoke exposure disrupts bacterial clearance and abolishes immune responses in muco-obstructive lung disease. J. Immunol. 199, 1170\u20131183. https:\/\/doi.org\/10.4049\/jimmunol.1700144 (2017).","journal-title":"J. Immunol."},{"key":"94843_CR17","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00381.2020","author":"I Choudhary","year":"2020","unstructured":"Choudhary, I., Vo, T., Paudel, K., Patial, S. & Saini, Y. Compartment-specific transcriptomics of ozone-exposed murine lungs reveals sex and cell type-associated perturbations relevant to mucoinflammatory lung diseases. Am. J. Physiol. Lung Cell Mol. Physiol. https:\/\/doi.org\/10.1152\/ajplung.00381.2020 (2020).","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"94843_CR18","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.4049\/jimmunol.1900234","volume":"204","author":"BW Lewis","year":"2020","unstructured":"Lewis, B. W. et al. Ablation of IL-33 suppresses Th2 responses but is accompanied by sustained mucus obstruction in the scnn1b transgenic mouse model. J. Immunol. 204, 1650\u20131660. https:\/\/doi.org\/10.4049\/jimmunol.1900234 (2020).","journal-title":"J. Immunol."},{"key":"94843_CR19","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin, J. et al. Fiji: An open-source platform for biological-image analysis. Nat. Methods 9, 676\u2013682. https:\/\/doi.org\/10.1038\/nmeth.2019 (2012).","journal-title":"Nat. Methods"},{"key":"94843_CR20","doi-asserted-by":"publisher","first-page":"1167","DOI":"10.1006\/bbrc.1993.2599","volume":"197","author":"DA Griffiths-Johnson","year":"1993","unstructured":"Griffiths-Johnson, D. A., Collins, P. D., Rossi, A. G., Jose, P. J. & Williams, T. J. The chemokine, eotaxin, activates guinea-pig eosinophils in vitro and causes their accumulation into the lung in vivo. Biochem. Biophys. Res. Commun. 197, 1167\u20131172. https:\/\/doi.org\/10.1006\/bbrc.1993.2599 (1993).","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"94843_CR21","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1084\/jem.182.4.1169","volume":"182","author":"PD Collins","year":"1995","unstructured":"Collins, P. D., Marleau, S., Griffiths-Johnson, D. A., Jose, P. J. & Williams, T. J. Cooperation between interleukin-5 and the chemokine eotaxin to induce eosinophil accumulation in vivo. J. Exp. Med. 182, 1169\u20131174. https:\/\/doi.org\/10.1084\/jem.182.4.1169 (1995).","journal-title":"J. Exp. Med."},{"key":"94843_CR22","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1016\/j.immuni.2005.09.015","volume":"23","author":"J Schmitz","year":"2005","unstructured":"Schmitz, J. et al. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 23, 479\u2013490. https:\/\/doi.org\/10.1016\/j.immuni.2005.09.015 (2005).","journal-title":"Immunity"},{"key":"94843_CR23","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/1074-7613(95)90047-0","volume":"2","author":"X Cao","year":"1995","unstructured":"Cao, X. et al. Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain. Immunity 2, 223\u2013238. https:\/\/doi.org\/10.1016\/1074-7613(95)90047-0 (1995).","journal-title":"Immunity"},{"key":"94843_CR24","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1016\/0092-8674(92)90030-g","volume":"68","author":"P Mombaerts","year":"1992","unstructured":"Mombaerts, P. et al. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68, 869\u2013877. https:\/\/doi.org\/10.1016\/0092-8674(92)90030-g (1992).","journal-title":"Cell"},{"key":"94843_CR25","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1002\/eji.201142018","volume":"42","author":"RG Klein Wolterink","year":"2012","unstructured":"Klein Wolterink, R. G. et al. Pulmonary innate lymphoid cells are major producers of IL-5 and IL-13 in murine models of allergic asthma. Eur. J. Immunol 42, 1106\u20131116. https:\/\/doi.org\/10.1002\/eji.201142018 (2012).","journal-title":"Eur. J. Immunol"},{"key":"94843_CR26","doi-asserted-by":"publisher","first-page":"758","DOI":"10.1016\/j.immuni.2014.04.013","volume":"40","author":"Y Motomura","year":"2014","unstructured":"Motomura, Y. et al. Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation. Immunity 40, 758\u2013771. https:\/\/doi.org\/10.1016\/j.immuni.2014.04.013 (2014).","journal-title":"Immunity"},{"key":"94843_CR27","doi-asserted-by":"publisher","first-page":"11489","DOI":"10.1073\/pnas.1003988107","volume":"107","author":"AE Price","year":"2010","unstructured":"Price, A. E. et al. Systemically dispersed innate IL-13-expressing cells in type 2 immunity. Proc. Natl. Acad. Sci. USA 107, 11489\u201311494. https:\/\/doi.org\/10.1073\/pnas.1003988107 (2010).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"94843_CR28","doi-asserted-by":"publisher","first-page":"10838","DOI":"10.1073\/pnas.94.20.10838","volume":"94","author":"N Noben-Trauth","year":"1997","unstructured":"Noben-Trauth, N. et al. An interleukin 4 (IL-4)-independent pathway for CD4+ T cell IL-4 production is revealed in IL-4 receptor-deficient mice. Proc. Natl. Acad. Sci. USA 94, 10838\u201310843. https:\/\/doi.org\/10.1073\/pnas.94.20.10838 (1997).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"94843_CR29","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1182\/blood.V96.2.719","volume":"96","author":"N Faust","year":"2000","unstructured":"Faust, N., Varas, F., Kelly, L. M., Heck, S. & Graf, T. Insertion of enhanced green fluorescent protein into the lysozyme gene creates mice with green fluorescent granulocytes and macrophages. Blood 96, 719\u2013726 (2000).","journal-title":"Blood"},{"key":"94843_CR30","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1038\/icb.2009.115","volume":"88","author":"LA Spencer","year":"2010","unstructured":"Spencer, L. A. & Weller, P. F. Eosinophils and Th2 immunity: Contemporary insights. Immunol. Cell Biol. 88, 250\u2013256. https:\/\/doi.org\/10.1038\/icb.2009.115 (2010).","journal-title":"Immunol. Cell Biol."},{"key":"94843_CR31","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1097\/ACI.0000000000000133","volume":"15","author":"R Divekar","year":"2015","unstructured":"Divekar, R. & Kita, H. Recent advances in epithelium-derived cytokines (IL-33, IL-25, and thymic stromal lymphopoietin) and allergic inflammation. Curr. Opin. Allergy Clin. Immunol. 15, 98\u2013103. https:\/\/doi.org\/10.1097\/ACI.0000000000000133 (2015).","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"94843_CR32","doi-asserted-by":"publisher","first-page":"1367","DOI":"10.1038\/nature08900","volume":"464","author":"DR Neill","year":"2010","unstructured":"Neill, D. R. et al. Nuocytes represent a new innate effector leukocyte that mediates type-2 immunity. Nature 464, 1367\u20131370. https:\/\/doi.org\/10.1038\/nature08900 (2010).","journal-title":"Nature"},{"key":"94843_CR33","doi-asserted-by":"publisher","first-page":"1823","DOI":"10.1084\/jem.20122332","volume":"210","author":"SA Saenz","year":"2013","unstructured":"Saenz, S. A. et al. IL-25 simultaneously elicits distinct populations of innate lymphoid cells and multipotent progenitor type 2 (MPPtype2) cells. J. Exp. Med. 210, 1823\u20131837. https:\/\/doi.org\/10.1084\/jem.20122332 (2013).","journal-title":"J. Exp. Med."},{"key":"94843_CR34","doi-asserted-by":"publisher","first-page":"1441","DOI":"10.1172\/JCI124606","volume":"129","author":"F Roan","year":"2019","unstructured":"Roan, F., Obata-Ninomiya, K. & Ziegler, S. F. Epithelial cell-derived cytokines: more than just signaling the alarm. J. Clin. Investig. 129, 1441\u20131451. https:\/\/doi.org\/10.1172\/JCI124606 (2019).","journal-title":"J. Clin. Investig."},{"key":"94843_CR35","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1165\/rcmb.2012-0080TR","volume":"47","author":"T Maes","year":"2012","unstructured":"Maes, T., Joos, G. F. & Brusselle, G. G. Targeting interleukin-4 in asthma: Lost in translation?. Am. J. Respir. Cell Mol. Biol. 47, 261\u2013270. https:\/\/doi.org\/10.1165\/rcmb.2012-0080TR (2012).","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"94843_CR36","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1146\/annurev-immunol-031210-101312","volume":"29","author":"W Ouyang","year":"2011","unstructured":"Ouyang, W., Rutz, S., Crellin, N. K., Valdez, P. A. & Hymowitz, S. G. Regulation and functions of the IL-10 family of cytokines in inflammation and disease. Annu. Rev. Immunol. 29, 71\u2013109. https:\/\/doi.org\/10.1146\/annurev-immunol-031210-101312 (2011).","journal-title":"Annu. Rev. Immunol."},{"key":"94843_CR37","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1146\/annurev.immunol.19.1.683","volume":"19","author":"KW Moore","year":"2001","unstructured":"Moore, K. W., de Waal Malefyt, R., Coffman, R. L. & O\u2019Garra, A. Interleukin-10 and the interleukin-10 receptor. Annu. Rev. Immunol. 19, 683\u2013765. https:\/\/doi.org\/10.1146\/annurev.immunol.19.1.683 (2001).","journal-title":"Annu. Rev. Immunol."},{"key":"94843_CR38","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1084\/jem.170.6.2081","volume":"170","author":"DF Fiorentino","year":"1989","unstructured":"Fiorentino, D. F., Bond, M. W. & Mosmann, T. R. Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones. J. Exp. Med. 170, 2081\u20132095. https:\/\/doi.org\/10.1084\/jem.170.6.2081 (1989).","journal-title":"J. Exp. Med."},{"key":"94843_CR39","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.immuni.2015.06.021","volume":"43","author":"H Morita","year":"2015","unstructured":"Morita, H. et al. An interleukin-33-mast cell-interleukin-2 axis suppresses papain-induced allergic inflammation by promoting regulatory T cell numbers. Immunity 43, 175\u2013186. https:\/\/doi.org\/10.1016\/j.immuni.2015.06.021 (2015).","journal-title":"Immunity"},{"key":"94843_CR40","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1073\/pnas.1315854111","volume":"111","author":"E Hams","year":"2014","unstructured":"Hams, E. et al. IL-25 and type 2 innate lymphoid cells induce pulmonary fibrosis. Proc. Natl. Acad. Sci. USA 111, 367\u2013372. https:\/\/doi.org\/10.1073\/pnas.1315854111 (2014).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"94843_CR41","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1073\/pnas.1206587110","volume":"110","author":"LY Hung","year":"2013","unstructured":"Hung, L. Y. et al. IL-33 drives biphasic IL-13 production for noncanonical type 2 immunity against hookworms. Proc. Natl. Acad. Sci. USA 110, 282\u2013287. https:\/\/doi.org\/10.1073\/pnas.1206587110 (2013).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"94843_CR42","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.jaci.2013.03.048","volume":"132","author":"TA Doherty","year":"2013","unstructured":"Doherty, T. A. et al. Lung type 2 innate lymphoid cells express cysteinyl leukotriene receptor 1, which regulates TH2 cytokine production. J. Allergy Clin. Immunol. 132, 205\u2013213. https:\/\/doi.org\/10.1016\/j.jaci.2013.03.048 (2013).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94843_CR43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003615","volume":"9","author":"SA Gorski","year":"2013","unstructured":"Gorski, S. A., Hahn, Y. S. & Braciale, T. J. Group 2 innate lymphoid cell production of IL-5 is regulated by NKT cells during influenza virus infection. PLoS Pathog. 9, e1003615. https:\/\/doi.org\/10.1371\/journal.ppat.1003615 (2013).","journal-title":"PLoS Pathog."},{"key":"94843_CR44","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004372","volume":"10","author":"KM Vannella","year":"2014","unstructured":"Vannella, K. M. et al. Incomplete deletion of IL-4Ralpha by LysM(Cre) reveals distinct subsets of M2 macrophages controlling inflammation and fibrosis in chronic schistosomiasis. PLoS Pathog. 10, e1004372. https:\/\/doi.org\/10.1371\/journal.ppat.1004372 (2014).","journal-title":"PLoS Pathog."},{"key":"94843_CR45","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1093\/intimm\/dxt003","volume":"25","author":"U Muller","year":"2013","unstructured":"Muller, U. et al. Abrogation of IL-4 receptor-alpha-dependent alternatively activated macrophages is sufficient to confer resistance against pulmonary cryptococcosis despite an ongoing T(h)2 response. Int. Immunol. 25, 459\u2013470. https:\/\/doi.org\/10.1093\/intimm\/dxt003 (2013).","journal-title":"Int. Immunol."},{"key":"94843_CR46","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1016\/j.jaci.2012.03.011","volume":"130","author":"NE Nieuwenhuizen","year":"2012","unstructured":"Nieuwenhuizen, N. E. et al. Allergic airway disease is unaffected by the absence of IL-4Ralpha-dependent alternatively activated macrophages. J. Allergy Clin. Immunol. 130, 743\u2013750. https:\/\/doi.org\/10.1016\/j.jaci.2012.03.011 (2012).","journal-title":"J. Allergy Clin. Immunol."},{"key":"94843_CR47","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1189\/jlb.71.6.1033","volume":"71","author":"MT Borchers","year":"2002","unstructured":"Borchers, M. T. et al. In vitro assessment of chemokine receptor-ligand interactions mediating mouse eosinophil migration. J. Leukoc. Biol. 71, 1033\u20131041 (2002).","journal-title":"J. Leukoc. Biol."},{"key":"94843_CR48","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1023\/a:1008942828960","volume":"8","author":"BE Clausen","year":"1999","unstructured":"Clausen, B. E., Burkhardt, C., Reith, W., Renkawitz, R. & Forster, I. Conditional gene targeting in macrophages and granulocytes using LysMcre mice. Transgenic Res. 8, 265\u2013277. https:\/\/doi.org\/10.1023\/a:1008942828960 (1999).","journal-title":"Transgenic Res."},{"key":"94843_CR49","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.jim.2014.05.009","volume":"408","author":"CL Abram","year":"2014","unstructured":"Abram, C. L., Roberge, G. L., Hu, Y. & Lowell, C. A. Comparative analysis of the efficiency and specificity of myeloid-Cre deleting strains using ROSA-EYFP reporter mice. J. Immunol. Methods 408, 89\u2013100. https:\/\/doi.org\/10.1016\/j.jim.2014.05.009 (2014).","journal-title":"J. Immunol. Methods"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94843-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94843-9","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94843-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,3]],"date-time":"2022-12-03T11:43:16Z","timestamp":1670067796000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-94843-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,29]]},"references-count":49,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["94843"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-94843-9","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,29]]},"assertion":[{"value":"9 November 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 July 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 July 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15465"}}