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In order to investigate the prevalence and genetic characteristics of <jats:italic>B. hominis<\/jats:italic> and <jats:italic>C. belli<\/jats:italic> in HIV\/AIDS patients, a total of 285 faecal samples were individually collected from HIV\/AIDS patients in Guangxi, China. <jats:italic>B. hominis<\/jats:italic> and <jats:italic>C. belli<\/jats:italic> were investigated by amplifying the barcode region of the SSU rRNA gene and the internal transcribed spacer 1 (ITS-1) region\u00a0of the rRNA gene, respectively. Chi-square test or Fisher\u2019s exact test were conducted to assess the risk factors related to <jats:italic>B. hominis<\/jats:italic> and <jats:italic>C. belli<\/jats:italic> infection. The prevalence of <jats:italic>B. hominis<\/jats:italic> and <jats:italic>C. belli<\/jats:italic> was 6.0% (17\/285) and 1.1% (3\/285) respectively. Four genotypes of <jats:italic>B. hominis<\/jats:italic> were detected, with ST3 (n\u2009=\u20098) and ST1 (n\u2009=\u20096) being predominant, followed by ST6 (n\u2009=\u20092) and ST7 (n\u2009=\u20091). Females had a statistically higher prevalence of <jats:italic>B. hominis<\/jats:italic> (11.6%) than males (4.2%)<jats:italic>.<\/jats:italic> The statistical analysis also showed that the prevalence of <jats:italic>B. hominis<\/jats:italic> was significantly associated with age group and educational level<jats:italic>.<\/jats:italic> Our study provides convincing evidence for the genetic diversity of <jats:italic>B. hominis<\/jats:italic>, which indicates its potential zoonotic transmission and is the first report on the molecular characteristics of <jats:italic>C. belli<\/jats:italic> in HIV\/AIDS patients in China.<\/jats:p>","DOI":"10.1038\/s41598-021-94962-3","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T10:02:50Z","timestamp":1628157770000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Prevalence and genetic characteristics of Blastocystis hominis and Cystoisospora belli in HIV\/AIDS patients in Guangxi Zhuang Autonomous Region, China"],"prefix":"10.1038","volume":"11","author":[{"given":"Ning","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zunfu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongsheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianping","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"94962_CR1","unstructured":"United Nations Programme on HIV\/AIDS Global HIV\/AIDS statistics: 2020 fact sheet. 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