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To evaluate the effect of the bacteriophage formulation on the treatment of gastrointestinal infection, rats were infected with <jats:italic>Salmonella enterica<\/jats:italic>, <jats:italic>Shigella flexneri<\/jats:italic>, and <jats:italic>Escherichia coli<\/jats:italic>. The rats were weighed and their stools cultured. The results showed that the group which had the chitosan encapsulated bacteriophage cocktail did not lose weight after 3\u00a0days and had significantly lower group weight changes. Weight loss was significant in the rats that had cefixime administered instead. Positive cultured stools were reduced after 4\u00a0days compared to 2\u00a0days in the treated group with the chitosan encapsulated bacteriophage cocktail. 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