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The retrospective study is to investigate the in vitro susceptibility to the antibiotics vancomycin, amikacin, and ceftazidime of bacterial endophthalmitis isolates in specimens at a tertiary referral center from January 1996 to April 2019 in Taiwan. Overall, 450 (49.9%) isolates were Gram positive, 447 (49.6%) were Gram negative, and 4 (0.4%) were Gram variable. In Gram-positive isolates, coagulase-negative staphylococci were the most commonly cultured bacteria (158, 35.1%), followed by\n                    <jats:italic>Streptococci<\/jats:italic>\n                    (100, 22.2%),\n                    <jats:italic>Enterococci<\/jats:italic>\n                    (75, 16.7%), and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (70, 15.6%). In Gram-negative isolates, they were\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (166, 37.1%) and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    (131, 29.3%). All Gram-positive organisms were susceptible to vancomycin, with the exception of one\n                    <jats:italic>Enterococcus faecium<\/jats:italic>\n                    isolate (1\/450, 0.2%). Of the Gram-negative isolates, 96.9% and 93.7% were susceptible to ceftazidime and amikacin, respectively. Nine isolates (9\/447, 2.0%) were multidrug-resistant Gram-negative bacteria, comprising\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    (4\/164, 2.4%),\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    (2\/3, 67%), and\n                    <jats:italic>Stenotrophomonas maltophilia<\/jats:italic>\n                    (3\/18, 17%). In conclusion, in vitro susceptibility testing revealed that vancomycin remains the suitable antibiotic treatment for Gram-positive endophthalmitis. Ceftazidime and amikacin provide approximately the same degree of Gram-negative coverage. Multidrug-resistant bacterial endophthalmitis was uncommon.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-95458-w","type":"journal-article","created":{"date-parts":[[2021,8,5]],"date-time":"2021-08-05T06:02:50Z","timestamp":1628143370000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Susceptibility of bacterial endophthalmitis isolates to vancomycin, ceftazidime, and amikacin"],"prefix":"10.1038","volume":"11","author":[{"given":"Kuan-Jen","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Hui","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiun-Ho","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung-Chi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen-Po","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan-Kai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Chi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hung-Da","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene Yu-Chuan","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi-Chun","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,8,5]]},"reference":[{"key":"95458_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1086\/595011","volume":"48","author":"HW Boucher","year":"2009","unstructured":"Boucher, H. 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