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In poultry,\n                    <jats:italic>C. gallinacea<\/jats:italic>\n                    infections appear asymptomatic, but studies about the pathogenic potential are limited. In this study two novel sequence types of\n                    <jats:italic>C. gallinacea<\/jats:italic>\n                    were isolated from apparently healthy chickens. Both isolates (NL_G47 and NL_F725) were closely related to each other and have at least 99.5% DNA sequence identity to\n                    <jats:italic>C. gallinacea<\/jats:italic>\n                    Type strain 08-1274\/3. To gain further insight into the pathogenic potential, infection experiments in embryonated chicken eggs and comparative genomics with\n                    <jats:italic>Chlamydia psittaci<\/jats:italic>\n                    were performed.\n                    <jats:italic>C. psittaci<\/jats:italic>\n                    is a ubiquitous zoonotic pathogen of birds and mammals, and infection in poultry can result in severe systemic illness. In experiments with embryonated chicken eggs,\n                    <jats:italic>C. gallinacea<\/jats:italic>\n                    induced mortality was observed, potentially strain dependent, but lower compared to\n                    <jats:italic>C. psittaci<\/jats:italic>\n                    induced mortality. Comparative analyses confirmed all currently available\n                    <jats:italic>C. gallinacea<\/jats:italic>\n                    genomes possess the hallmark genes coding for known and potential virulence factors as found in\n                    <jats:italic>C. psittaci<\/jats:italic>\n                    albeit to a reduced number of orthologues or paralogs. The presence of potential virulence factors and the observed mortality in embryonated eggs indicates\n                    <jats:italic>C. gallinacea<\/jats:italic>\n                    should rather be considered as an opportunistic pathogen than an innocuous commensal.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-95966-9","type":"journal-article","created":{"date-parts":[[2021,8,13]],"date-time":"2021-08-13T06:03:06Z","timestamp":1628834586000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Genetic and phenotypic analysis of the pathogenic potential of two novel Chlamydia gallinacea strains compared to Chlamydia psittaci"],"prefix":"10.1038","volume":"11","author":[{"given":"Marloes","family":"Heijne","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Jelocnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Umanets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S. 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