{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:45:31Z","timestamp":1791596731613,"version":"4.3.4"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100017026","name":"Spastic Paraplegia Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017026","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A central need for neurodegenerative diseases is to find curative drugs for the many clinical subtypes, the causative gene for most cases being unknown. This requires the classification of disease cases at the genetic and cellular level, an understanding of disease aetiology in the subtypes and the development of phenotypic assays for high throughput screening of large compound libraries. Herein we describe a method that facilitates these requirements based on cell morphology that is being increasingly used as a readout defining cell state. In patient-derived fibroblasts we quantified 124 morphological features in 100,000 cells from 15 people with two genotypes (<jats:italic>SPAST<\/jats:italic> and <jats:italic>SPG7<\/jats:italic>) of Hereditary Spastic Paraplegia (HSP) and matched controls. Using machine learning analysis, we distinguished between each genotype and separated them from controls. Cell morphologies changed with treatment with noscapine, a tubulin-binding drug, in a genotype-dependent manner, revealing a novel effect on one of the genotypes (<jats:italic>SPG7<\/jats:italic>). These findings demonstrate a method for morphological profiling in fibroblasts, an accessible non-neural cell, to classify and distinguish between clinical subtypes of neurodegenerative diseases, for drug discovery, and potentially for biomarkers of disease severity and progression.<\/jats:p>","DOI":"10.1038\/s41598-021-95995-4","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T10:05:02Z","timestamp":1629194702000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Single cell morphology distinguishes genotype and drug effect in Hereditary Spastic Paraplegia"],"prefix":"10.1038","volume":"11","author":[{"given":"Gautam","family":"Wali","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shlomo","family":"Berkovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Whiten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Mackay-Sim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn M.","family":"Sue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,17]]},"reference":[{"key":"95995_CR1","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1242\/dmm.023002","volume":"8","author":"G Abrahamsen","year":"2015","unstructured":"Abrahamsen, G. et al. A patient-derived stem cell model of hereditary spastic paraplegia with SPAST mutations. Dis. Model Mech. 8, 1339. https:\/\/doi.org\/10.1242\/dmm.023002 (2015).","journal-title":"Dis. Model Mech."},{"key":"95995_CR2","doi-asserted-by":"publisher","first-page":"826","DOI":"10.1002\/ana.24633","volume":"79","author":"HK Mishra","year":"2016","unstructured":"Mishra, H. K. et al. GSK3ss-dependent dysregulation of neurodevelopment in SPG11-patient induced pluripotent stem cell model. Ann. Neurol. 79, 826\u2013840. https:\/\/doi.org\/10.1002\/ana.24633 (2016).","journal-title":"Ann. Neurol."},{"key":"95995_CR3","doi-asserted-by":"publisher","first-page":"4859","DOI":"10.1093\/hmg\/ddu200","volume":"23","author":"F Perez-Branguli","year":"2014","unstructured":"Perez-Branguli, F. et al. Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia. Hum. Mol. Genet. 23, 4859\u20134874. https:\/\/doi.org\/10.1093\/hmg\/ddu200 (2014).","journal-title":"Hum. 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