{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T22:10:10Z","timestamp":1788473410579,"version":"build-2803163510"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T00:00:00Z","timestamp":1629763200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004532","name":"Japan International Cooperation Agency","doi-asserted-by":"publisher","award":["02-P-9427\/2018"],"award-info":[{"award-number":["02-P-9427\/2018"]}],"id":[{"id":"10.13039\/501100004532","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Agency for Medical Research and Development","award":["JP21jm0110015"],"award-info":[{"award-number":["JP21jm0110015"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Infections due to triazole-resistant\n                    <jats:italic>Aspergillus fumigatus<\/jats:italic>\n                    are increasingly reported worldwide and are associated with treatment failure and mortality. The principal class of azole-resistant isolates is characterized by tandem repeats of 34\u00a0bp or 46\u00a0bp within the promoter region of the\n                    <jats:italic>cyp51A<\/jats:italic>\n                    gene. Loop-mediated isothermal amplification (LAMP) is a widely used nucleic acid amplification system that is fast and specific. Here we describe a LAMP assay method to detect the 46\u00a0bp tandem repeat insertion in the\n                    <jats:italic>cyp51A<\/jats:italic>\n                    gene promoter region based on novel LAMP primer sets\n                    <jats:italic>.<\/jats:italic>\n                    It also differentiated strains with TR\n                    <jats:sub>46<\/jats:sub>\n                    tandem repeats from those with TR\n                    <jats:sub>34<\/jats:sub>\n                    tandem repeats. These results showed this TR\n                    <jats:sub>46<\/jats:sub>\n                    -LAMP method is specific, rapid, and provides crucial insights to develop novel antifungal therapeutic strategies against severe fungal infections due to\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    with TR\n                    <jats:sub>46<\/jats:sub>\n                    tandem repeats.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-96651-7","type":"journal-article","created":{"date-parts":[[2021,8,24]],"date-time":"2021-08-24T06:05:20Z","timestamp":1629785120000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Development and validation of LAMP primer sets for rapid identification of Aspergillus fumigatus carrying the cyp51A TR46 azole resistance gene"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0588-4859","authenticated-orcid":false,"given":"Plinio","family":"Trabasso","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuhiro","family":"Matsuzawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teppei","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Hagiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzuru","family":"Mikami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luiza","family":"Moretti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,24]]},"reference":[{"key":"96651_CR1","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.mib.2018.02.005","volume":"45","author":"NM Revie","year":"2018","unstructured":"Revie, N. M., Lyer, K. R., Robbins, N. & Cowen, L. E. Antifungal drug resistance: evolution, mechanisms and impact. Curr. Opin. Microbiol. 45, 70\u201376 (2018).","journal-title":"Curr. Opin. Microbiol."},{"key":"96651_CR2","doi-asserted-by":"publisher","first-page":"697","DOI":"10.2217\/fmb.14.27","volume":"9","author":"A Chowdhary","year":"2014","unstructured":"Chowdhary, A., Sharma, C., Hagen, F. & Meis, J. F. Exploring azole antifungal drug resistance in Aspergillus fumigatus with particular reference to resistance mechanisms. Future Microbiol. 9, 697\u2013711 (2014).","journal-title":"Future Microbiol."},{"key":"96651_CR3","doi-asserted-by":"publisher","first-page":"1064","DOI":"10.15585\/mmwr.mm6738a5","volume":"67","author":"KD Beer","year":"2018","unstructured":"Beer, K. D. et al. Multidrug-resistant Aspergillus fumigatus carrying mutations linked to environmental fungicide exposure: Three states, 2010\u20132017. MMWR Morb. Mortal Wkly. Rep. 67, 1064\u20131067 (2018).","journal-title":"MMWR Morb. Mortal Wkly. Rep."},{"key":"96651_CR4","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1093\/cid\/ciw444","volume":"63","author":"TE Patterson","year":"2016","unstructured":"Patterson, T. E. et al. Practice guidelines for the diagnosis and management of aspergillosis: 2016 update by the Infectious Diseases Society of America. Clin. Infect. Dis. 63, 438\u2013442 (2016).","journal-title":"Clin. Infect. Dis."},{"key":"96651_CR5","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.3389\/fmicb.2016.01382","volume":"7","author":"D Hagiwara","year":"2016","unstructured":"Hagiwara, D. et al. Epidemiological and genomic landscape of azole resistance mechanisms in Aspergillus fungi. Front. Microbiol. 7, 1382 (2016).","journal-title":"Front. Microbiol."},{"issue":"5957","key":"96651_CR6","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1126\/science.326.5957.1173","volume":"326","author":"M Enserink","year":"2009","unstructured":"Enserink, M. Infectious diseases: Farm fungicides linked to resistance in a human pathogen. Science 326(5957), 1173. https:\/\/doi.org\/10.1126\/science.326.5957.1173 (2009).","journal-title":"Science"},{"key":"96651_CR7","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1183\/13993003.00583-2015","volume":"47","author":"DW Denning","year":"2016","unstructured":"Denning, D. W. et al. Chronic pulmonary aspergillosis: rationale and clinical guidelines for diagnosis and management. Eur. Respir. J. 47, 45\u201368 (2016).","journal-title":"Eur. Respir. J."},{"issue":"7","key":"96651_CR8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppt.1005775.eCollection","volume":"12","author":"F Gsaller","year":"2016","unstructured":"Gsaller, F. et al. Sterol biosynthesis and azole tolerance is governed by the opposing actions of SrbA and the CCAT binding complex. PLOS Pathog. 12(7), e1005775. https:\/\/doi.org\/10.1371\/journal.ppt.1005775.eCollection (2016).","journal-title":"PLOS Pathog."},{"key":"96651_CR9","doi-asserted-by":"publisher","first-page":"4924","DOI":"10.1111\/1462-2920.15274","volume":"22","author":"AV Nywening","year":"2020","unstructured":"Nywening, A. V., Rybak, J. M., Rogers, P. D. & Fortwendel, J. R. Mechanisms of triazole resistance in Aspergillus fumigatus. Environ. Microbiol. 22, 4924\u20134952 (2020).","journal-title":"Environ. Microbiol."},{"key":"96651_CR10","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1093\/jac\/dku364","volume":"70","author":"J Van Ingen","year":"2015","unstructured":"Van Ingen, J. et al. Azole, polyene and echinocandin, MIC distributions for wild-type, TR34\/L98H and TR46\/Y121F\/T289A Aspergillus fumigatus isolates in the Netherlands. J. Antimicrob. Chemother. 70, 178\u2013181 (2015).","journal-title":"J. Antimicrob. Chemother."},{"key":"96651_CR11","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1128\/JCM.02478-15","volume":"54","author":"PW Nathan","year":"2016","unstructured":"Nathan, P. W. et al. First detection of TR34 L98H and TR46 Y121F T289A cyp51 mutations in Aspergillus fumigatus isolated in the United States. J. Clin. Microbiol. 54, 168\u2013171 (2016).","journal-title":"J. Clin. Microbiol."},{"issue":"8","key":"96651_CR12","doi-asserted-by":"publisher","first-page":"e00270","DOI":"10.1128\/AAC.00270-17","volume":"61","author":"S R\u00f6\u00dfler","year":"2017","unstructured":"R\u00f6\u00dfler, S. et al. Progressive dispersion of azole resistance in Aspergillus fumigatus: Fatal invasive aspergillosis in a patient with acute myeloid leukemia infected with an A. fumigatus strain with a cyp51A TR46 Y121FM1721 T289A allele. Antimicrob. 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Efficient identification of clinical relevant Candida yeast species by use of an assay combining panfungal loop-mediated isothermal DNA amplification with hybridization to species-specific oligonucleotide probes. J. Clin. Microbiol. 46, 713\u2013729 (2008).","journal-title":"J. Clin. Microbiol."},{"key":"96651_CR16","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1111\/j.1574-695X.2011.00785.x","volume":"62","author":"F Patrice","year":"2011","unstructured":"Patrice, F. et al. Rubustness of a loop-nediated isothermal amplification reaction for diaganostic applification. FEMS Immunol. Med. Microbiol. 62, 41\u201348 (2011).","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"96651_CR17","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1111\/1348-0421.12734","volume":"63","author":"K Shirato","year":"2019","unstructured":"Shirato, K. Detecting amplification of loop-mediated isothermal amplification. Microbiol. 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