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Here we quantify corneal biomechanical properties in an adult classic EDS mouse model using two different measurement approaches suited for murine corneal mechanical characterization and relate differences to stromal structure using Second Harmonic Generation (SHG) microscopy. Quasi-static Optical Coherence Elastography (OCE) was conducted non-invasively during ambient pressure modulation by\u2009\u2212\u20093\u00a0mmHg. 2D-extensometry measurements was conducted invasively consisting of a pre-conditioning cycle, a stress-relaxation test and a rupture test. In a total of 28 eyes from a <jats:italic>Col5a1<\/jats:italic><jats:sup>+<jats:italic>\/\u2212<\/jats:italic><\/jats:sup> mouse model and wild-type C57BL\/6 littermates (wt), <jats:italic>Col5a1<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> corneas were thinner when compared to wt, (125\u2009\u00b1\u200911 vs 148\u2009\u00b1\u200910\u00a0\u03bcm, respectively, p\u2009&lt;\u20090.001). Short-term elastic modulus was significantly increased in OCE (506\u2009\u00b1\u200988 vs 430\u2009\u00b1\u2009103\u00a0kPa, p\u2009=\u20090.023), and the same trend was observed in 2D-extensometry (30.7\u2009\u00b1\u200912.1\u00a0kPa vs 21.5\u2009\u00b1\u20095.7, p\u2009=\u20090.057). In contrast, in stress relaxation tests, <jats:italic>Col5a1<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> corneas experienced a stronger relaxation (55% vs 50%, p\u2009=\u20090.01). SHG microscopy showed differences in forward and backward scattered signal indicating abnormal collagen fibrils in <jats:italic>Col5a1<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> corneas. We propose that disturbed collagen fibril structure in <jats:italic>Col5a1<\/jats:italic><jats:sup>+\/\u2212<\/jats:sup> corneas affects the viscoelastic properties. Results presented here support clinical findings, in which thin corneas with global ultrastructural alterations maintain a normal corneal shape.<\/jats:p>","DOI":"10.1038\/s41598-021-96775-w","type":"journal-article","created":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T10:02:57Z","timestamp":1630317777000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Collagen V insufficiency in a mouse model for Ehlers Danlos-syndrome affects viscoelastic biomechanical properties explaining thin and brittle corneas"],"prefix":"10.1038","volume":"11","author":[{"given":"Sabine","family":"Kling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio A.","family":"Torres-Netto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hormoz","family":"Abdshahzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar M.","family":"Espana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad","family":"Hafezi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,30]]},"reference":[{"key":"96775_CR1","doi-asserted-by":"publisher","first-page":"1795","DOI":"10.1016\/j.jcrs.2004.05.002","volume":"30","author":"K Pesudovs","year":"2004","unstructured":"Pesudovs, K. Orbscan mapping in Ehlers\u2013Danlos syndrome. J. Cataract Refract. Surg. 30, 1795\u20131798 (2004).","journal-title":"J. Cataract Refract. Surg."},{"key":"96775_CR2","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1167\/iovs.05-0771","volume":"47","author":"F Segev","year":"2006","unstructured":"Segev, F. et al. Structural abnormalities of the cornea and lid resulting from collagen V mutations. Investig. Ophthalmol. Vis. Sci. 47, 565\u2013573 (2006).","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"96775_CR3","doi-asserted-by":"publisher","first-page":"8062","DOI":"10.1167\/iovs.13-12837","volume":"54","author":"E Villani","year":"2013","unstructured":"Villani, E. et al. The cornea in classic type Ehlers\u2013Danlos syndrome: Macro-and microstructural changes. Investig. Ophthalmol. Vis. Sci. 54, 8062\u20138068 (2013).","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"96775_CR4","doi-asserted-by":"crossref","unstructured":"Espana, E. M. & Birk, D. E. Composition, structure and function of the corneal stroma. Exp. Eye Res. 108137 (2020).","DOI":"10.1016\/j.exer.2020.108137"},{"key":"96775_CR5","doi-asserted-by":"publisher","first-page":"999","DOI":"10.1083\/jcb.106.3.999","volume":"106","author":"DE Birk","year":"1988","unstructured":"Birk, D. E., Fitch, J. M., Babiarz, J. P. & Linsenmayer, T. F. Collagen type I and type V are present in the same fibril in the avian corneal stroma. J. Cell Biol. 106, 999\u20131008 (1988).","journal-title":"J. Cell Biol."},{"key":"96775_CR6","first-page":"1470","volume":"27","author":"D Birk","year":"1986","unstructured":"Birk, D., Fitch, J. & Linsenmayer, T. Organization of collagen types I and V in the embryonic chicken cornea. Investig. Ophthalmol. Vis. Sci. 27, 1470\u20131477 (1986).","journal-title":"Investig. Ophthalmol. Vis. 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