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This study aimed to investigate the interaction of <jats:italic>S. aureus<\/jats:italic> and <jats:italic>P. aeruginosa<\/jats:italic>, which alters iron-related sigma factors regulation and antibiotic resistance. The antibiotic resistance of <jats:italic>P. aeruginosa<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> was investigated in a L929 cell culture model. The expression level of <jats:italic>pvdS<\/jats:italic>, <jats:italic>hasI<\/jats:italic> (<jats:italic>P. aeruginosa<\/jats:italic> sigma factors), and <jats:italic>sigS<\/jats:italic> (<jats:italic>S. aureus<\/jats:italic> sigma factor) genes was determined using Quantitative Real-Time PCR. <jats:italic>pvdS<\/jats:italic> and <jats:italic>hasI<\/jats:italic> were downregulated during co-culture with <jats:italic>S. aureus<\/jats:italic>, while the susceptibility to carbapenems increased (p-value\u2009&lt;\u20090.0001). Also, there was a direct significant relationship between resistance to vancomycin with <jats:italic>sigS<\/jats:italic>. Regarding the findings of the current study, iron-related sigma factors of <jats:italic>P. aeruginosa<\/jats:italic> and <jats:italic>S. aureus<\/jats:italic> play a role in induction susceptibility to various antibiotics, including carbapenems and vancomycin.<\/jats:p>","DOI":"10.1038\/s41598-021-98017-5","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T10:02:41Z","timestamp":1631786561000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9257-4479","authenticated-orcid":false,"given":"Hamed","family":"Tahmasebi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6932-1633","authenticated-orcid":false,"given":"Sanaz","family":"Dehbashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9991-8193","authenticated-orcid":false,"given":"Mohammad Reza","family":"Arabestani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"98017_CR1","first-page":"127","volume":"21","author":"H Tahmasebi","year":"2019","unstructured":"Tahmasebi, H., Maleki, F., Dehbashi, S. & Arabestani, M. 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