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In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with \u03b2-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the\n                    <jats:italic>\u0394hns<\/jats:italic>\n                    mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of\n                    <jats:italic>adeFGH<\/jats:italic>\n                    . The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes\n                    <jats:italic>aidA, abaI, kar<\/jats:italic>\n                    and\n                    <jats:italic>fadD<\/jats:italic>\n                    were up-regulated in\n                    <jats:italic>\u0394hns<\/jats:italic>\n                    mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of\n                    <jats:italic>hns<\/jats:italic>\n                    to combat\n                    <jats:italic>A. baumannii<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1038\/s41598-021-98101-w","type":"journal-article","created":{"date-parts":[[2021,9,16]],"date-time":"2021-09-16T06:03:34Z","timestamp":1631772214000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii"],"prefix":"10.1038","volume":"11","author":[{"given":"Deja","family":"Rodgers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casin","family":"Le","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Pimentel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisel R.","family":"Tuttobene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1s","family":"Subils","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Escalante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora Garc\u00eda","family":"Vescovi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Sieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Bonomo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo E.","family":"Tolmasky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Soledad","family":"Ramirez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,16]]},"reference":[{"key":"98101_CR1","doi-asserted-by":"publisher","first-page":"3628","DOI":"10.1128\/AAC.00284-09","volume":"53","author":"MD Adams","year":"2009","unstructured":"Adams, M. 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