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In this study, biosynthesis of zinc oxide nanoparticles (ZnO NPs) were carried out using leaf extracts of <jats:italic>Phoenix dactylifera L.<\/jats:italic> and Zinc nitrate. The effect of ZnO nanoparticles on biomass and biochemical parameters was investigated. Biosynthesized ZnO nanostructure was characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), UV\u2013visible spectrophotometer and Fourier transform infrared spectroscopy (FTIR). Which resulted in spherical shape with size ranging between 16 to 35\u00a0nm of Biosynthesized ZnO nanoparticles and UV absorption beak at 370.5\u00a0nm with clear peaks of functional groups. The impact of different concentrations (0.0\u00a0mg\/L, 80\u00a0mg\/L and 160\u00a0mg\/L) of biosynthesized ZnO nanoparticles on biomass and bioactive compounds production of <jats:italic>Juniperus procera <\/jats:italic>in vitro was investigated. The results showed that, biosynthesized ZnO NPs (80\u00a0mg\/L and 160\u00a0mg\/L) concentrations were boosted the growth of <jats:italic>J. Procera<\/jats:italic> with significantly compared to non-treated plants in vitro. The highest concentration (160\u00a0mg\/L) of ZnO NPs was enhanced the growth of plant at beginning period, one month later shoots became yellow and callus turned to be brownish. Moreover, the influence of ZnO NPs on phytochemical compounds in callus of <jats:italic>Juniperus procera<\/jats:italic> was examined using GC\u2013MS analysis. The differences among treatments were recoded<jats:bold>.<\/jats:bold> Overall, zinc oxide nanoparticles substantially improved the growth of shoots and callus with increasing of biochemical parameters such as chlorophyll a, total phenolic and flavonoids contents, besides the total protein and, SOD, CAT and APX activity. ZnO NPs might be induced some phytochemical compounds as well as inhibit.<\/jats:p>","DOI":"10.1038\/s41598-021-98607-3","type":"journal-article","created":{"date-parts":[[2021,9,27]],"date-time":"2021-09-27T10:20:00Z","timestamp":1632738000000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":76,"title":["Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera"],"prefix":"10.1038","volume":"11","author":[{"given":"Abdalrhaman M.","family":"Salih","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fahad","family":"Al-Qurainy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salim","family":"Khan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Tarroum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Nadeem","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassan O.","family":"Shaikhaldein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdel-Rhman Zakaria","family":"Gaafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norah S.","family":"Alfarraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,27]]},"reference":[{"key":"98607_CR1","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/B978-0-12-804304-2.00006-8","volume-title":"Nutrient Delivery","author":"MA V\u00e9lez","year":"2017","unstructured":"V\u00e9lez, M. 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