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Increased production of inflammatory factors such as cytokines has been implicated in axon destruction. In the present study, we compared the expression level of <jats:italic>IL7R<\/jats:italic>, <jats:italic>NFATc2<\/jats:italic>, and <jats:italic>RNF213<\/jats:italic> genes in the peripheral blood of 72 MS patients (37 familial MS, 35 sporadic MS) and 74 healthy controls (34 individuals with a family history of the disease, 40 healthy controls without a family history) via Real-time PCR. Our results showed that the expression level of <jats:italic>IL7R<\/jats:italic> was decreased in the sporadic patients in comparison with other groups. Additionally, there was an increased <jats:italic>NFATc2<\/jats:italic> expression level in MS patients versus healthy controls. Increased expression of <jats:italic>NFATc2<\/jats:italic> in sporadic and familial groups compared to the controls, and familial group versus FDR was also seen. Our results also represented an increased expression level of <jats:italic>RNF213<\/jats:italic> in familial patients as compared to the control group. The similar <jats:italic>RNF213<\/jats:italic> expression between sporadic and control group, as well as FDR and familial group was also seen. Diagnostic evaluation was performed by receiver operating characteristic (ROC) curve analysis and area under the curve (AUC) calculation. The correlation of clinical parameters including onset age and Expanded Disability Status Scale (EDSS) with our gene expression levels were also assessed. Overall, decreased expression level of <jats:italic>IL7R<\/jats:italic> in the sporadic cases and increased expression level of <jats:italic>NFATc2<\/jats:italic> may be associated with the pathogenesis of MS disease. Confirmation of the effects of differential expression of <jats:italic>RNF213<\/jats:italic> gene requires further studies in the wider statistical populations.<\/jats:p>","DOI":"10.1038\/s41598-021-98691-5","type":"journal-article","created":{"date-parts":[[2021,9,28]],"date-time":"2021-09-28T10:03:21Z","timestamp":1632823401000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Expression and clinical significance of IL7R, NFATc2, and RNF213 in familial and sporadic multiple sclerosis"],"prefix":"10.1038","volume":"11","author":[{"given":"Seyedeh Zahra Hosseini","family":"Imani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohreh","family":"Hojati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheyda","family":"Khalilian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fariba","family":"Dehghanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Kheirollahi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Khorrami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Shaygannejad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omid","family":"Mirmosayyeb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,9,28]]},"reference":[{"key":"98691_CR1","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1007\/s12031-016-0851-3","volume":"61","author":"N Ehtesham","year":"2017","unstructured":"Ehtesham, N., Khorvash, F. & Kheirollahi, M. miR-145 and miR20a-5p potentially mediate pleiotropic effects of interferon-beta through mitogen-activated protein kinase signaling pathway in multiple sclerosis patients. 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