{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:40:22Z","timestamp":1791909622085,"version":"4.3.4"},"reference-count":69,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T00:00:00Z","timestamp":1633996800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T00:00:00Z","timestamp":1633996800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Maintenance of genomic stability is critical to prevent diseases such as cancer. As such, eukaryotic cells have multiple pathways to efficiently detect, signal and repair DNA damage. One common form of exogenous DNA damage comes from ultraviolet B (UVB) radiation. UVB generates cyclobutane pyrimidine dimers (CPD) that must be rapidly detected and repaired to maintain the genetic code. The nucleotide excision repair (NER) pathway is the main repair system for this type of DNA damage. Here, we determined the role of the human Single-Stranded DNA Binding protein 2, hSSB2, in the response to UVB exposure. We demonstrate that hSSB2 levels increase in vitro and in vivo after UVB irradiation and that hSSB2 rapidly binds to chromatin. Depletion of hSSB2 results in significantly decreased Replication Protein A (RPA32) phosphorylation and impaired RPA32 localisation to the site of UV-induced DNA damage. Delayed recruitment of NER protein Xeroderma Pigmentosum group C (XPC) was also observed, leading to increased cellular sensitivity to UVB. Finally, hSSB2 was shown to have affinity for single-strand DNA containing a single CPD and for duplex DNA with a two-base mismatch mimicking a CPD moiety. Altogether our data demonstrate that hSSB2 is involved in the cellular response to UV exposure.<\/jats:p>","DOI":"10.1038\/s41598-021-99355-0","type":"journal-article","created":{"date-parts":[[2021,10,12]],"date-time":"2021-10-12T09:24:52Z","timestamp":1634030692000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["hSSB2 (NABP1) is required for the recruitment of RPA during the cellular response to DNA UV damage"],"prefix":"10.1038","volume":"11","author":[{"given":"Didier","family":"Boucher","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruvini","family":"Kariawasam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Burgess","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Gimenez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tristan E.","family":"Ocampo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blake","family":"Ferguson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Naqi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme J.","family":"Walker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Bolderson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Gamsjaeger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth J.","family":"O\u2019Byrne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liza","family":"Cubeddu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kum Kum","family":"Khanna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek J.","family":"Richard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,10,12]]},"reference":[{"key":"99355_CR1","doi-asserted-by":"publisher","first-page":"1713","DOI":"10.1039\/c5pp00185d","volume":"14","author":"C Nishisgori","year":"2015","unstructured":"Nishisgori, C. Current concept of photocarcinogenesis. Photochem. Photobiol. Sci. 14, 1713\u20131721 (2015).","journal-title":"Photochem. Photobiol. Sci."},{"key":"99355_CR2","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1111\/j.1365-2133.2007.08108.x","volume":"157","author":"M Yaar","year":"2007","unstructured":"Yaar, M. & Gilchrest, B. A. Photoageing: Mechanism, prevention and therapy. Br. J. Dermatol. 157, 874\u2013887 (2007).","journal-title":"Br. J. Dermatol."},{"key":"99355_CR3","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1111\/php.12685","volume":"93","author":"T Douki","year":"2017","unstructured":"Douki, T., von Koschembahr, A. & Cadet, J. Insight in DNA repair of UV-induced pyrimidine dimers by chromatographic methods. Photochem. Photobiol. 93, 207\u2013215 (2017).","journal-title":"Photochem. Photobiol."},{"key":"99355_CR4","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1269\/jrr.10175","volume":"52","author":"H Ikehata","year":"2011","unstructured":"Ikehata, H. & Ono, T. The mechanisms of UV mutagenesis. J. Radiat. Res. 52, 115\u2013125 (2011).","journal-title":"J. Radiat. Res."},{"key":"99355_CR5","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1111\/php.12368","volume":"91","author":"J Cadet","year":"2015","unstructured":"Cadet, J., Douki, T. & Ravanat, J. L. Oxidatively generated damage to cellular DNA by UVB and UVA radiation. Photochem. Photobiol. 91, 140\u2013155 (2015).","journal-title":"Photochem. Photobiol."},{"key":"99355_CR6","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1111\/php.12668","volume":"93","author":"J Hu","year":"2017","unstructured":"Hu, J. & Adar, S. The Cartography of UV-induced DNA damage formation and DNA repair. Photochem. Photobiol. 93, 199\u2013206 (2017).","journal-title":"Photochem. Photobiol."},{"key":"99355_CR7","doi-asserted-by":"publisher","first-page":"23","DOI":"10.32607\/20758251-2014-6-1-23-34","volume":"6","author":"IO Petruseva","year":"2014","unstructured":"Petruseva, I. O., Evdokimov, A. N. & Lavrik, O. I. Molecular mechanism of global genome nucleotide excision repair. Acta Naturae 6, 23\u201334 (2014).","journal-title":"Acta Naturae"},{"key":"99355_CR8","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/j.dnarep.2016.05.015","volume":"44","author":"K Sugasawa","year":"2016","unstructured":"Sugasawa, K. Molecular mechanisms of DNA damage recognition for mammalian nucleotide excision repair. DNA Repair 44, 110\u2013117 (2016).","journal-title":"DNA Repair"},{"key":"99355_CR9","doi-asserted-by":"publisher","first-page":"a012625","DOI":"10.1101\/cshperspect.a012625","volume":"5","author":"W Vermeulen","year":"2013","unstructured":"Vermeulen, W. & Fousteri, M. Mammalian transcription-coupled excision repair. Cold Spring Harb. Perspect. Biol. 5, a012625 (2013).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"99355_CR10","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1007\/s13193-014-0307-6","volume":"5","author":"R Halkud","year":"2014","unstructured":"Halkud, R. et al. Xeroderma pigmentosum: Clinicopathological review of the multiple oculocutaneous malignancies and complications. Indian J. Surg. Oncol. 5, 120\u2013124 (2014).","journal-title":"Indian J. Surg. Oncol."},{"key":"99355_CR11","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1007\/s12105-016-0707-8","volume":"10","author":"JO Black","year":"2016","unstructured":"Black, J. O. Xeroderma pigmentosum. Head Neck Pathol. 10, 139\u2013144 (2016).","journal-title":"Head Neck Pathol."},{"key":"99355_CR12","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1242\/jcs.035105","volume":"122","author":"B Shiotani","year":"2009","unstructured":"Shiotani, B. & Zou, L. ATR signaling at a glance. J. Cell Sci. 122, 301\u2013304 (2009).","journal-title":"J. Cell Sci."},{"key":"99355_CR13","doi-asserted-by":"publisher","first-page":"28036","DOI":"10.1074\/jbc.M704635200","volume":"282","author":"J Lee","year":"2007","unstructured":"Lee, J., Kumagai, A. & Dunphy, W. G. The Rad9-Hus1-Rad1 checkpoint clamp regulates interaction of TopBP1 with ATR. J. Biol. Chem. 282, 28036\u201328044 (2007).","journal-title":"J. Biol. Chem."},{"key":"99355_CR14","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1073\/pnas.0510223103","volume":"103","author":"Y Namiki","year":"2006","unstructured":"Namiki, Y. & Zou, L. ATRIP associates with replication protein A-coated ssDNA through multiple interactions. Proc. Natl. Acad. Sci. USA. 103, 580\u2013585 (2006).","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"99355_CR15","doi-asserted-by":"publisher","first-page":"3681","DOI":"10.1111\/febs.13387","volume":"282","author":"VAJ Smits","year":"2015","unstructured":"Smits, V. A. J. & Gillespie, D. A. DNA damage control: Regulation and functions of checkpoint kinase 1. FEBS J. 282, 3681\u20133692 (2015).","journal-title":"FEBS J."},{"key":"99355_CR16","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/cr.2014.147","volume":"25","author":"A Mar\u00e9chal","year":"2015","unstructured":"Mar\u00e9chal, A. & Zou, L. RPA-coated single-stranded DNA as a platform for post-translational modifications in the DNA damage response. Cell Res. 25, 9\u201323 (2015).","journal-title":"Cell Res."},{"key":"99355_CR17","doi-asserted-by":"publisher","first-page":"39517","DOI":"10.1074\/jbc.M605121200","volume":"281","author":"E Olson","year":"2006","unstructured":"Olson, E., Nievera, C. J., Klimovich, V., Fanning, E. & Wu, X. RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint. J. Biol. Chem. 281, 39517\u201339533 (2006).","journal-title":"J. Biol. Chem."},{"key":"99355_CR18","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1091\/mbc.12.5.1199","volume":"12","author":"GG Oakley","year":"2001","unstructured":"Oakley, G. G. et al. UV-induced hyperphosphorylation of replication protein a depends on DNA replication and expression of ATM protein. Mol. Biol. Cell 12, 1199\u20131213 (2001).","journal-title":"Mol. Biol. Cell"},{"key":"99355_CR19","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1146\/annurev.biochem.66.1.61","volume":"66","author":"MS Wold","year":"1997","unstructured":"Wold, M. S. Replication protein A: A heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism. Annu. Rev. Biochem. 66, 61\u201392 (1997).","journal-title":"Annu. Rev. Biochem."},{"key":"99355_CR20","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1146\/annurev.bi.63.070194.002523","volume":"63","author":"TM Lohman","year":"1994","unstructured":"Lohman, T. M. & Ferrari, M. E. Escherichia coli single-stranded DNA-binding protein: Multiple DNA-binding modes and cooperativities. Annu. Rev. Biochem. 63, 527\u2013570 (1994).","journal-title":"Annu. Rev. Biochem."},{"key":"99355_CR21","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1128\/mr.54.4.342-380.1990","volume":"54","author":"RR Meyer","year":"1990","unstructured":"Meyer, R. R. & Laine, P. S. The single-stranded DNA-binding protein of Escherichia coli. Microbiol. Rev. 54, 342\u2013380 (1990).","journal-title":"Microbiol. Rev."},{"key":"99355_CR22","doi-asserted-by":"publisher","first-page":"13827","DOI":"10.1073\/pnas.2336100100","volume":"100","author":"L Zou","year":"2003","unstructured":"Zou, L., Liu, D. & Elledge, S. J. Replication protein A-mediated recruitment and activation of Rad17 complexes. Proc. Natl. Acad. Sci. USA. 100, 13827\u201313832 (2003).","journal-title":"Proc. Natl. Acad. Sci. USA."},{"key":"99355_CR23","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1080\/10409230902849180","volume":"44","author":"DJ Richard","year":"2009","unstructured":"Richard, D. J., Bolderson, E. & Khanna, K. K. Multiple human single-stranded DNA binding proteins function in genome maintenance: Structural, biochemical and functional analysis. Crit. Rev. Biochem. Mol. Biol. 44, 98\u2013116 (2009).","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"99355_CR24","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1002\/j.1460-2075.1993.tb05726.x","volume":"12","author":"AG Murzin","year":"1993","unstructured":"Murzin, A. G. OB(oligonucleotide\/oligosaccharide binding)-fold: Common structural and functional solution for non-homologous sequences. EMBO J. 12, 861\u2013867 (1993).","journal-title":"EMBO J."},{"key":"99355_CR25","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1038\/nsb0397-161","volume":"4","author":"D Suck","year":"1997","unstructured":"Suck, D. Common fold, common function, common origin?. Nat. Struct. Biol. 4, 161\u2013165 (1997).","journal-title":"Nat. Struct. Biol."},{"key":"99355_CR26","doi-asserted-by":"publisher","first-page":"1542","DOI":"10.1126\/science.1083430","volume":"300","author":"L Zou","year":"2003","unstructured":"Zou, L. & Elledge, S. J. Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science 300, 1542\u20131548 (2003).","journal-title":"Science"},{"key":"99355_CR27","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1080\/10409239991209255","volume":"34","author":"C Iftode","year":"1999","unstructured":"Iftode, C., Daniely, Y. & Borowiec, J. A. Replication protein A (RPA): The eukaryotic SSB. Crit. Rev. Biochem. Mol. Biol. 34, 141\u2013180 (1999).","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"99355_CR28","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/j.jmb.2005.08.050","volume":"353","author":"L Cubeddu","year":"2005","unstructured":"Cubeddu, L. & White, M. F. DNA damage detection by an archaeal single-stranded DNA-binding protein. J. Mol. Biol. 353, 507\u2013516 (2005).","journal-title":"J. Mol. Biol."},{"key":"99355_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/mc.10143","volume":"38","author":"BS Thoma","year":"2003","unstructured":"Thoma, B. S. & Vasquez, K. M. Critical DNA damage recognition functions of XPC-hHR23B and XPA-RPA in nucleotide excision repair. Mol. Carcinog. 38, 1\u201313 (2003).","journal-title":"Mol. Carcinog."},{"key":"99355_CR30","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1016\/S0021-9258(17)43529-0","volume":"259","author":"JW Chase","year":"1984","unstructured":"Chase, J. W., L\u2019Italien, J. J., Murphy, J. B., Spicer, E. K. & Williams, K. R. Characterization of the Escherichia coli SSB-113 mutant single-stranded DNA-binding protein. Cloning of the gene, DNA and protein sequence analysis, high pressure liquid chromatography peptide mapping, and DNA-binding studies. J. Biol. Chem. 259, 805\u2013814 (1984).","journal-title":"J. Biol. Chem."},{"key":"99355_CR31","doi-asserted-by":"publisher","first-page":"677","DOI":"10.1038\/nature06883","volume":"453","author":"DJ Richard","year":"2008","unstructured":"Richard, D. J. et al. Single-stranded DNA-binding protein hSSB1 is critical for genomic stability. Nature 453, 677\u2013682 (2008).","journal-title":"Nature"},{"key":"99355_CR32","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1083\/jcb.200907026","volume":"187","author":"JR Skaar","year":"2009","unstructured":"Skaar, J. R. et al. INTS3 controls the hSSB1-mediated DNA damage response. J. Cell Biol. 187, 25\u201332 (2009).","journal-title":"J. Cell Biol."},{"key":"99355_CR33","doi-asserted-by":"publisher","first-page":"23525","DOI":"10.1074\/jbc.C109.039586","volume":"284","author":"Y Li","year":"2009","unstructured":"Li, Y. et al. HSSB1 and hSSB2 form similar multiprotein complexes that participate in DNA damage response. J. Biol. Chem. 284, 23525\u201323531 (2009).","journal-title":"J. Biol. Chem."},{"key":"99355_CR34","doi-asserted-by":"publisher","first-page":"384","DOI":"10.1016\/j.molcel.2009.06.011","volume":"35","author":"J Huang","year":"2009","unstructured":"Huang, J., Gong, Z., Ghosal, G. & Chen, J. SOSS complexes participate in the maintenance of genomic stability. Mol. Cell 35, 384\u2013393 (2009).","journal-title":"Mol. Cell"},{"key":"99355_CR35","first-page":"1","volume":"1","author":"E Bolderson","year":"2014","unstructured":"Bolderson, E. et al. Human single-stranded DNA binding protein 1 (hSSB1\/NABP2) is required for the stability and repair of stalled replication forks. Nucleic Acids Res. 1, 1\u201311 (2014).","journal-title":"Nucleic Acids Res."},{"key":"99355_CR36","doi-asserted-by":"publisher","first-page":"8817","DOI":"10.1093\/nar\/gkv790","volume":"43","author":"N Paquet","year":"2015","unstructured":"Paquet, N. et al. hSSB1 (NABP2\/ OBFC2B) is required for the repair of 8-oxo-guanine by the hOGG1-mediated base excision repair pathway. Nucleic Acids Res. 43, 8817\u20138829 (2015).","journal-title":"Nucleic Acids Res."},{"key":"99355_CR37","doi-asserted-by":"publisher","first-page":"27446","DOI":"10.1038\/srep27446","volume":"6","author":"N Paquet","year":"2016","unstructured":"Paquet, N. et al. hSSB1 (NABP2\/OBFC2B) is regulated by oxidative stress. Sci. Rep. 6, 27446 (2016).","journal-title":"Sci. Rep."},{"key":"99355_CR38","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1038\/cr.2013.31","volume":"23","author":"P Gu","year":"2013","unstructured":"Gu, P., Deng, W., Lei, M. & Chang, S. Single strand DNA binding proteins 1 and 2 protect newly replicated telomeres. Cell Res. 23, 705\u2013719 (2013).","journal-title":"Cell Res."},{"key":"99355_CR39","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1038\/cr.2015.19","volume":"25","author":"JR Skaar","year":"2015","unstructured":"Skaar, J. R. et al. The Integrator complex controls the termination of transcription at diverse classes of gene targets. Cell Res. 25, 288\u2013305 (2015).","journal-title":"Cell Res."},{"key":"99355_CR40","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv369","author":"A Kar","year":"2015","unstructured":"Kar, A. et al. RPA70 depletion induces hSSB1\/2-INTS3 complex to initiate ATR signaling. Nucleic Acids Res. https:\/\/doi.org\/10.1093\/nar\/gkv369 (2015).","journal-title":"Nucleic Acids Res."},{"key":"99355_CR41","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.dnarep.2017.03.006","volume":"54","author":"NW Ashton","year":"2017","unstructured":"Ashton, N. W. et al. hSSB1 phosphorylation is dynamically regulated by DNA-PK and PPP-family protein phosphatases. DNA Repair 54, 30\u201339 (2017).","journal-title":"DNA Repair"},{"key":"99355_CR42","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1016\/j.bbagen.2015.11.003","volume":"1860","author":"V Vidhyasagar","year":"2016","unstructured":"Vidhyasagar, V. et al. C-termini are essential and distinct for nucleic acid binding of human NABP1 and NABP2. Biochim. Biophys. Acta Gen. Subj. 1860, 371\u2013383 (2016).","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"99355_CR43","doi-asserted-by":"publisher","first-page":"e1003298","DOI":"10.1371\/journal.pgen.1003298","volume":"9","author":"W Shi","year":"2013","unstructured":"Shi, W. et al. Essential developmental, genomic stability, and tumour suppressor functions of the mouse orthologue of hSSB1\/NABP2. PLoS Genet. 9, e1003298 (2013).","journal-title":"PLoS Genet."},{"key":"99355_CR44","doi-asserted-by":"publisher","first-page":"4045","DOI":"10.1038\/emboj.2012.247","volume":"31","author":"N Feldhahn","year":"2012","unstructured":"Feldhahn, N. et al. The hSSB1 orthologue Obfc2b is essential for skeletogenesis but dispensable for the DNA damage response in vivo. EMBO J. 31, 4045\u20134056 (2012).","journal-title":"EMBO J."},{"key":"99355_CR45","doi-asserted-by":"publisher","first-page":"30408","DOI":"10.1074\/jbc.M109.039404","volume":"284","author":"F Zhang","year":"2009","unstructured":"Zhang, F., Wu, J. & Yu, X. Integrator3, a partner of single-stranded DNA-binding protein 1, participates in the DNA damage response. J. Biol. Chem. 284, 30408\u201330415 (2009).","journal-title":"J. Biol. Chem."},{"key":"99355_CR46","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1016\/j.celrep.2014.02.020","volume":"6","author":"W Ren","year":"2014","unstructured":"Ren, W. et al. Structural basis of SOSS1 complex assembly and recognition of ssDNA. Cell Rep. 6, 982\u2013991 (2014).","journal-title":"Cell Rep."},{"key":"99355_CR47","doi-asserted-by":"publisher","first-page":"3326","DOI":"10.1096\/fj.14-269944","volume":"29","author":"D Boucher","year":"2015","unstructured":"Boucher, D. et al. Ssb2\/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice. FASEB J. 29, 3326\u20133334 (2015).","journal-title":"FASEB J."},{"key":"99355_CR48","doi-asserted-by":"publisher","first-page":"2471","DOI":"10.1182\/blood-2016-06-725093","volume":"129","author":"W Shi","year":"2017","unstructured":"Shi, W. et al. Ssb1 and Ssb2 cooperate to regulate mouse hematopoietic stem and progenitor cells by resolving replicative stress. Blood 129, 2471\u20132478 (2017).","journal-title":"Blood"},{"key":"99355_CR49","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1002\/prot.25806","volume":"88","author":"T Lawson","year":"2020","unstructured":"Lawson, T. et al. The structural details of the interaction of single-stranded DNA binding protein hSSB2 (NABP1\/OBFC2A) with UV-damaged DNA. Proteins Struct. Funct. Bioinform. 88, 319\u2013326 (2020).","journal-title":"Proteins Struct. Funct. Bioinform."},{"key":"99355_CR50","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/s12867-017-0090-3","volume":"18","author":"LV Croft","year":"2017","unstructured":"Croft, L. V. et al. hSSB1 associates with and promotes stability of the BLM helicase. BMC Mol. Biol. 18, 13 (2017).","journal-title":"BMC Mol. Biol."},{"key":"99355_CR51","first-page":"1","volume":"35","author":"P Mukhopadhyay","year":"2015","unstructured":"Mukhopadhyay, P., Ferguson, B., Muller, H. K., Handoko, H. Y. & Walker, G. J. Murine melanomas accelerated by a single UVR exposure carry photoproduct footprints but lack UV signature C>T mutations in critical genes. Oncogene 35, 1\u20139 (2015).","journal-title":"Oncogene"},{"key":"99355_CR52","doi-asserted-by":"publisher","first-page":"3550","DOI":"10.1038\/emboj.2012.193","volume":"31","author":"C Godon","year":"2012","unstructured":"Godon, C. et al. Generation of DNA single-strand displacement by compromised nucleotide excision repair. EMBO J. 31, 3550\u20133563 (2012).","journal-title":"EMBO J."},{"key":"99355_CR53","doi-asserted-by":"publisher","first-page":"3873","DOI":"10.1093\/nar\/20.15.3873","volume":"20","author":"D Coverley","year":"1992","unstructured":"Coverley, D., Kenny, M. K., Lane, D. P. & Wood, R. D. A role for the human single-stranded DNA binding protein HSSB\/RPA in an early stage of nucleotide excision repair. Nucleic Acids Res. 20, 3873\u20133880 (1992).","journal-title":"Nucleic Acids Res."},{"key":"99355_CR54","doi-asserted-by":"publisher","first-page":"883","DOI":"10.2741\/3652","volume":"15","author":"GG Oakley","year":"2010","unstructured":"Oakley, G. G. & Patrick, S. M. Replication protein A: Directing traffic at the intersection of replication and repair. Front. Biosci. 15, 883\u2013900 (2010).","journal-title":"Front. Biosci."},{"key":"99355_CR55","doi-asserted-by":"publisher","first-page":"15325","DOI":"10.1074\/jbc.M412870200","volume":"280","author":"JB Robbins","year":"2005","unstructured":"Robbins, J. B. et al. The euryarchaeota, nature\u2019s medium for engineering of single-stranded DNA-binding proteins. J. Biol. Chem. 280, 15325\u201315339 (2005).","journal-title":"J. Biol. Chem."},{"key":"99355_CR56","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.ab.2013.04.030","volume":"440","author":"R Gamsjaeger","year":"2013","unstructured":"Gamsjaeger, R. et al. Semiquantitative and quantitative analysis of protein-DNA interactions using steady-state measurements in surface plasmon resonance competition experiments. Anal. Biochem. 440, 178\u2013185 (2013).","journal-title":"Anal. Biochem."},{"key":"99355_CR57","doi-asserted-by":"publisher","first-page":"727","DOI":"10.2174\/1389203716666150505230806","volume":"16","author":"JK Flores","year":"2015","unstructured":"Flores, J. K. et al. Biophysical characterisation and quantification of nucleic acid-protein interactions: EMSA, MST and SPR. Curr. Protein Pept. Sci. 16, 727\u2013734 (2015).","journal-title":"Curr. Protein Pept. Sci."},{"key":"99355_CR58","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1042\/BJ20051781","volume":"397","author":"HS Kang","year":"2006","unstructured":"Kang, H. S. et al. NABP1, a novel RORgamma-regulated gene encoding a single-stranded nucleic-acid-binding protein. Biochem. J. 397, 89\u201399 (2006).","journal-title":"Biochem. J."},{"key":"99355_CR59","doi-asserted-by":"publisher","first-page":"1260419","DOI":"10.1126\/science.1260419","volume":"347","author":"M Uhlen","year":"2015","unstructured":"Uhlen, M. et al. Tissue-based map of the human proteome. Science 347, 1260419 (2015).","journal-title":"Science"},{"key":"99355_CR60","doi-asserted-by":"publisher","first-page":"2696","DOI":"10.1128\/MCB.20.8.2696-2705.2000","volume":"20","author":"G Rodrigo","year":"2000","unstructured":"Rodrigo, G., Roumagnac, S., Wold, M. S., Salles, B. & Calsou, P. DNA replication but not nucleotide excision repair is required for UVC-induced replication protein A phosphorylation in mammalian cells. Mol. Cell. Biol. 20, 2696\u20132705 (2000).","journal-title":"Mol. Cell. Biol."},{"key":"99355_CR61","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.dnarep.2016.05.007","volume":"44","author":"Z Livneh","year":"2016","unstructured":"Livneh, Z. et al. High-resolution genomic assays provide insight into the division of labor between TLS and HDR in mammalian replication of damaged DNA. DNA Repair 44, 59\u201367 (2016).","journal-title":"DNA Repair"},{"key":"99355_CR62","doi-asserted-by":"publisher","first-page":"5717","DOI":"10.1093\/nar\/gkw280","volume":"44","author":"A Quinet","year":"2016","unstructured":"Quinet, A. et al. Translesion synthesis mechanisms depend on the nature of DNA damage in UV-irradiated human cells. Nucleic Acids Res. 44, 5717\u20135731 (2016).","journal-title":"Nucleic Acids Res."},{"key":"99355_CR63","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.dnarep.2018.08.017","volume":"71","author":"A Ait Saada","year":"2018","unstructured":"Ait Saada, A., Lambert, S. A. E. & Carr, A. M. Preserving replication fork integrity and competence via the homologous recombination pathway. DNA Repair 71, 135\u2013147 (2018).","journal-title":"DNA Repair"},{"key":"99355_CR64","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1046\/j.1523-1747.2003.12450.x","volume":"121","author":"LC Young","year":"2003","unstructured":"Young, L. C., Hays, J. B., Tron, V. A. & Andrew, S. E. DNA mismatch repair proteins: Potential guardians against genomic instability and tumorigenesis induced by ultraviolet photoproducts. J. Investig. Dermatol. 121, 435\u2013440 (2003).","journal-title":"J. Investig. Dermatol."},{"key":"99355_CR65","first-page":"50","volume":"61","author":"K Nara","year":"2001","unstructured":"Nara, K. et al. Highly elevated ultraviolet-induced mutation frequency in isolated Chinese hamster cell lines defective in nucleotide excision repair and mismatch repair proteins. Cancer Res. 61, 50\u201352 (2001).","journal-title":"Cancer Res."},{"key":"99355_CR66","doi-asserted-by":"publisher","first-page":"5074","DOI":"10.1074\/jbc.M113.542787","volume":"289","author":"LA Lindsey-Boltz","year":"2014","unstructured":"Lindsey-Boltz, L. A. et al. Coupling of human DNA excision repair and the DNA damage checkpoint in a defined in vitro system. J. Biol. Chem. 289, 5074\u20135082 (2014).","journal-title":"J. Biol. Chem."},{"key":"99355_CR67","doi-asserted-by":"publisher","first-page":"36123","DOI":"10.1074\/jbc.M112.407825","volume":"287","author":"LA Lindsey-Boltz","year":"2012","unstructured":"Lindsey-Boltz, L. A., Reardon, J. T., Wold, M. S. & Sancar, A. In vitro analysis of the role of replication protein A (RPA) and RPA phosphorylation in ATR-mediated checkpoint signaling. J. Biol. Chem. 287, 36123\u201336131 (2012).","journal-title":"J. Biol. Chem."},{"key":"99355_CR68","doi-asserted-by":"publisher","first-page":"e109108","DOI":"10.1371\/journal.pone.0109108","volume":"9","author":"S Joseph","year":"2014","unstructured":"Joseph, S. et al. The structure of an LIM-only protein 4 (LMO4) and deformed epidermal autoregulatory factor-1 (DEAF1) complex reveals a common mode of binding to LMO4. PLoS ONE 9, e109108 (2014).","journal-title":"PLoS ONE"},{"key":"99355_CR69","doi-asserted-by":"publisher","first-page":"178","DOI":"10.1016\/j.ab.2013.04.030","volume":"440","author":"R Gamsjaeger","year":"2013","unstructured":"Gamsjaeger, R. et al. Semiquantitative and quantitative analysis of protein\u2013DNA interactions using steady-state measurements in surface plasmon resonance competition experiments. Anal. Biochem. 440, 178\u2013185 (2013).","journal-title":"Anal. Biochem."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-99355-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-99355-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-99355-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T08:58:22Z","timestamp":1706173102000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-021-99355-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,12]]},"references-count":69,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,12]]}},"alternative-id":["99355"],"URL":"https:\/\/doi.org\/10.1038\/s41598-021-99355-0","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,12]]},"assertion":[{"value":"24 February 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 September 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 October 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare the following competing financial interests; K.J.O. and D.J.R. are founders of CARP Pharmaceuticals. E.B., K.J.O. and D.J.R. are founders of Carpe Vitae Pharmaceuticals. All other authors have no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"20256"}}