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Elucidating the mechanisms of prion-like transmission of \u03b1-synuclein is important for the development of therapies for these diseases, but little is known about the details. Here, we injected \u03b1-synuclein fibrils into the brains of wild-type mice and examined the early phase of the induction of phosphorylated \u03b1-synuclein accumulation. We found that phosphorylated \u03b1-synuclein appeared within a few days after the intracerebral injection. It was observed initially in presynaptic regions and subsequently extended its localization to axons and cell bodies.\u3000These results suggest that extracellular \u03b1-synuclein fibrils are taken up into the presynaptic region and seed-dependently convert the endogenous normal \u03b1-synuclein that is abundant there to an abnormal phosphorylated form, which is then transported through the axon to the cell body.<\/jats:p>","DOI":"10.1038\/s41598-022-04780-4","type":"journal-article","created":{"date-parts":[[2022,1,21]],"date-time":"2022-01-21T11:02:41Z","timestamp":1642762961000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Phosphorylation of endogenous \u03b1-synuclein induced by extracellular seeds initiates at the pre-synaptic region and spreads to the cell body"],"prefix":"10.1038","volume":"12","author":[{"given":"Shiori","family":"Awa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genjiro","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Masuda-Suzukake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Nonaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Hasegawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,21]]},"reference":[{"key":"4780_CR1","doi-asserted-by":"crossref","unstructured":"Goedert, M. 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