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In plants, LCBs are linked to cell death processes and the regulation of defence reactions against pathogens, but their role in directly influencing growth of plant-interacting microorganisms has received little attention. Therefore, we tested the major plant LCB phytosphingosine in in vitro tests with the plant pathogenic fungi <jats:italic>Verticillium longisporum<\/jats:italic>, <jats:italic>Fusarium graminearum<\/jats:italic> and <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>, the plant symbiotic fungal endophyte <jats:italic>Serendipita indica<\/jats:italic>, the bacterial pathogens <jats:italic>Pseudomonas syringae<\/jats:italic> pv. <jats:italic>tomato<\/jats:italic> (<jats:italic>Pst<\/jats:italic>), <jats:italic>Agrobacterium tumefaciens<\/jats:italic>, and the related beneficial strain <jats:italic>Rhizobium radiobacter<\/jats:italic>. Phytosphingosine inhibited growth of these organisms at micromolar concentrations. Among the fungal pathogens, <jats:italic>S. sclerotiorum<\/jats:italic> was the most, and <jats:italic>F. graminearum<\/jats:italic> was the least sensitive. 15.9\u00a0\u03bcg\/mL phytosphingosine effectively killed 95% of the three bacterial species. Plant disease symptoms and growth of <jats:italic>Pst<\/jats:italic> were also inhibited by phytosphingosine when co-infiltrated into Arabidopsis leaves, with no visible negative effect on host tissue. Taken together, we demonstrate that the plant LCB phytosphingosine inhibits growth of plant-interacting microorganisms. We discuss the potential of elevated LCB levels to enhance plant pathogen resistance.<\/jats:p>","DOI":"10.1038\/s41598-022-05083-4","type":"journal-article","created":{"date-parts":[[2022,1,20]],"date-time":"2022-01-20T11:04:47Z","timestamp":1642676687000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["The major plant sphingolipid long chain base phytosphingosine inhibits growth of bacterial and fungal plant pathogens"],"prefix":"10.1038","volume":"12","author":[{"given":"Ren\u00e9","family":"Glenz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Kaiping","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delia","family":"G\u00f6pfert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Weber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Lambour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marvin","family":"Sylvester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Fr\u00f6schel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin J.","family":"Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Osman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Waller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,1,20]]},"reference":[{"key":"5083_CR1","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.mib.2017.12.011","volume":"43","author":"SL Heaver","year":"2018","unstructured":"Heaver, S. 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