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In this work, we developed a device for performing real-time colorimetric LAMP combining the accuracy of lab-based quantitative analysis with the simplicity of point-of-care testing. The device innovation lies on the use of a plastic tube anchored vertically on a hot surface while the side walls are exposed to a mini camera able to take snapshots of the colour change in real time during LAMP amplification. Competitive features are the rapid analysis (&lt;\u200930\u00a0min), quantification over 9 log-units, crude sample-compatibility (saliva, tissue, swabs), low detection limit (&lt;\u20095 copies\/reaction), smartphone-operation, fast prototyping (3D-printing) and ability to select the dye of interest (Phenol red, HNB). The device\u2019s clinical utility is demonstrated in cancer mutations-analysis during the detection of 0.01% of\n                    <jats:italic>BRAF<\/jats:italic>\n                    -V600E-to-wild-type molecules from tissue samples and COVID-19 testing with 97% (Ct\u2009&lt;\u200936.8) and 98% (Ct\u2009&lt;\u200930) sensitivity when using extracted RNA and nasopharyngeal-swabs, respectively. The device high technology-readiness-level makes it a suitable platform for performing any colorimetric LAMP assay; moreover, its simple and inexpensive fabrication holds promise for fast deployment and application in global diagnostics.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-06632-7","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T06:04:59Z","timestamp":1646719499000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":125,"title":["Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples"],"prefix":"10.1038","volume":"12","author":[{"given":"G.","family":"Papadakis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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