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Here we show that viral load of SARS-CoV-2 Ribonucleic Acid (RNA) in participants\u2019 naso-pharyngeal (NP) swabs positively correlated with RNA viral load they emitted in both droplets >10\n \n \n $$\\upmu \\hbox {m}$$<\/jats:tex-math>\n \n \n \u03bc<\/mml:mi>\n m<\/mml:mtext>\n <\/mml:mrow>\n <\/mml:math>\n <\/jats:alternatives>\n <\/jats:inline-formula>\n and bioaerosols <10\n \n \n $$\\upmu \\hbox {m}$$<\/jats:tex-math>\n \n \n \u03bc<\/mml:mi>\n m<\/mml:mtext>\n <\/mml:mrow>\n <\/mml:math>\n <\/jats:alternatives>\n <\/jats:inline-formula>\n directly captured during the combined expiratory activities of breathing, speaking and coughing using a standardized protocol, although the NP swabs had\n \n \n $$\\approx$$<\/jats:tex-math>\n \n \u2248<\/mml:mo>\n <\/mml:math>\n <\/jats:alternatives>\n <\/jats:inline-formula>\n 10\n \n \n $$^3\\times$$<\/jats:tex-math>\n \n \n \n \n 3<\/mml:mn>\n <\/mml:msup>\n \u00d7<\/mml:mo>\n <\/mml:mrow>\n <\/mml:math>\n <\/jats:alternatives>\n <\/jats:inline-formula>\n more RNA on average. By identifying highly-infectious individuals (maximum of 18,000 PFU\/mL in NP), we retrieved higher numbers of SARS-CoV-2 RNA gene copies in bioaerosol samples (maximum of 4.8\n \n \n $${\\times }10^{5}$$<\/jats:tex-math>\n \n \n \u00d7<\/mml:mo>\n \n 10<\/mml:mn>\n 5<\/mml:mn>\n <\/mml:msup>\n <\/mml:mrow>\n <\/mml:math>\n <\/jats:alternatives>\n <\/jats:inline-formula>\n gene copies\/mL and minimum cycle threshold of 26.2) relative to other studies. However, all attempts to identify infectious virus in size-segregated droplets and bioaerosols were negative by plaque assay (0 of 58). This outcome is partly attributed to the insufficient amount of viral material in each sample (as indicated by SARS-CoV-2 gene copies) or may indicate no infectious virus was present in such samples, although other possible factors are identified.\n <\/jats:p>","DOI":"10.1038\/s41598-022-07301-5","type":"journal-article","created":{"date-parts":[[2022,3,3]],"date-time":"2022-03-03T06:05:35Z","timestamp":1646287535000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Viral load of SARS-CoV-2 in droplets and bioaerosols directly captured during breathing, speaking and coughing"],"prefix":"10.1038","volume":"12","author":[{"given":"Tyler J.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert T.","family":"Nishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashlesha P.","family":"Sonpar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chan James","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberley A.","family":"Watson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie W.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Conly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David H.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason S.","family":"Olfert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,3]]},"reference":[{"key":"7301_CR1","unstructured":"World Health Organization. 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He also holds a contract from Singletto Inc. concerning methods for inactivating SARS-CoV-2. J.M.C. holds grants from the Canadian Institutes for Health Research on acute and primary care preparedness for COVID 19 in Alberta, Canada and was the primary local Investigator for a Staphylococcus aureus vaccine study funded by Pfizer for which all funding was provided only to the University of Calgary. He is a co-investigator on a WHO funded study using integrated human factors and ethnography approaches to identify and scale innovative IPC guidance implementation supports in primary care with a focus on low resource settings and using drone aerial systems to deliver medical supplies and PPE to remote First Nations communities during the COVID 19 pandemic. He also received support from the Centers for Disease Control and Prevention (CDC) to attend an Infection Control Think Tank Meeting. He is a member and Chair of the WHO Infection Prevention and Control Research and Development Expert Group for COVID 19 and a member of the WHO Health Emergencies Programme (WHE) Ad hoc COVID 19 IPC Guidance Development Group, both of which provide multidisciplinary advice to the WHO, for which no funding is received and from which no funding recommendations are made for any WHO contracts or grants. He is also a member of the Cochrane Acute Respiratory Infections Group. The remaining authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3484"}}