{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T20:11:25Z","timestamp":1789157485187,"version":"build-2803163510"},"reference-count":70,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T00:00:00Z","timestamp":1646179200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T00:00:00Z","timestamp":1646179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K09898 to Mohammad Zakir Hossain"],"award-info":[{"award-number":["20K09898 to Mohammad Zakir Hossain"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19K05871 to Hiroshi Ando"],"award-info":[{"award-number":["19K05871 to Hiroshi Ando"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K11656 to Junichi Kitagawa"],"award-info":[{"award-number":["17K11656 to Junichi Kitagawa"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We examined the role of TRPA1s in triggering the swallowing reflex. TRPA1s predominantly localized on thin nerve fibers and fibroblast-like cells in swallowing-related regions and on small to medium-sized superior laryngeal nerve-afferents in the nodose\u2013petrosal\u2013jugular ganglionic complex. Topical application of a TRPA1 agonist, allyl isothiocyanate (AITC), dose-dependently triggered swallowing reflexes. Prior topical application of a TRPA1 antagonist significantly attenuated the AITC-induced reflexes. Application of cold AITC (4\u00a0\u00b0C) very briefly reduced the on-site temperature to\u2009&lt;\u200917\u00a0\u00b0C (temperature at which TRPA1s can be activated), but had no effect on triggering of the reflex. By contrast, reducing the on-site temperature to\u2009&lt;\u200917\u00a0\u00b0C for a longer time by continuous flow of cold AITC or by application of iced AITC paradoxically delayed\/prevented the triggering of AITC-induced reflexes. Prior application of the TRPA1 antagonist had no effect on the threshold for the punctate mechanical stimuli-induced reflex or the number of low-force or high-force continuous mechanical pressure stimuli-induced reflexes. TRPA1s are functional and act as chemosensors, but not as cold sensors or mechanosensors, for triggering of the swallowing reflex. A brief cold stimulus has no effect on triggering of the reflex. However, a longer cold stimulus delays\/prevents triggering of the reflex because of cold anesthesia.<\/jats:p>","DOI":"10.1038\/s41598-022-07400-3","type":"journal-article","created":{"date-parts":[[2022,3,2]],"date-time":"2022-03-02T11:02:54Z","timestamp":1646218974000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["TRPA1s act as chemosensors but not as cold sensors or mechanosensors to trigger the swallowing reflex in rats"],"prefix":"10.1038","volume":"12","author":[{"given":"Mohammad Zakir","family":"Hossain","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Ando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumpei","family":"Unno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Kitagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,2]]},"reference":[{"key":"7400_CR1","doi-asserted-by":"publisher","first-page":"929","DOI":"10.1152\/physrev.2001.81.2.929","volume":"81","author":"A Jean","year":"2001","unstructured":"Jean, A. Brain stem control of swallowing: Neuronal network and cellular mechanisms. Physiol. Rev. 81, 929\u2013969 (2001).","journal-title":"Physiol. Rev."},{"key":"7400_CR2","doi-asserted-by":"publisher","first-page":"1786","DOI":"10.1248\/bpb.33.1786","volume":"33","author":"K Yamamura","year":"2010","unstructured":"Yamamura, K. et al. Neural mechanisms of swallowing and effects of taste and other stimuli on swallow initiation. Biol. Pharm. Bull. 33, 1786\u20131790 (2010).","journal-title":"Biol. Pharm. Bull."},{"key":"7400_CR3","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1007\/s00455-010-9301-5","volume":"25","author":"CM Steele","year":"2010","unstructured":"Steele, C. M. & Miller, A. J. Sensory input pathways and mechanisms in swallowing: a review. Dysphagia 25, 323\u2013333 (2010).","journal-title":"Dysphagia"},{"key":"7400_CR4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3390\/ijms21176214","volume":"21","author":"MZ Hossain","year":"2020","unstructured":"Hossain, M. Z., Ando, H., Unno, S. & Kitagawa, J. Targeting chemosensory ion channels in peripheral swallowing-related regions for the management of oropharyngeal dysphagia. Int. J. Mol. Sci. 21, 1\u201345 (2020).","journal-title":"Int. J. Mol. Sci."},{"key":"7400_CR5","doi-asserted-by":"publisher","first-page":"R1342","DOI":"10.1152\/ajpregu.00556.2001","volume":"282","author":"J-I Kitagawa","year":"2002","unstructured":"Kitagawa, J.-I., Shingai, T., Takahashi, Y. & Yamada, Y. Pharyngeal branch of the glossopharyngeal nerve plays a major role in reflex swallowing from the pharynx. Am. J. Physiol. Integr. Comp. Physiol. 282, R1342\u2013R1347 (2002).","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"7400_CR6","doi-asserted-by":"publisher","first-page":"1260","DOI":"10.1152\/ajplegacy.1971.221.5.1260","volume":"221","author":"WJ Sinclair","year":"1971","unstructured":"Sinclair, W. J. Role of the pharyngeal plexus in initiation of swallowing. Am. J. Physiol. 221, 1260\u20131263 (1971).","journal-title":"Am. J. Physiol."},{"key":"7400_CR7","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1002\/(SICI)1097-0185(199812)252:4<646::AID-AR15>3.0.CO;2-E","volume":"252","author":"I Sanders","year":"1998","unstructured":"Sanders, I. & Mu, L. Anatomy of the human internal superior laryngeal nerve. Anat. Rec. 252, 646\u2013656 (1998).","journal-title":"Anat. Rec."},{"key":"7400_CR8","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/0014-4886(68)90079-4","volume":"20","author":"AT Storey","year":"1968","unstructured":"Storey, A. T. Laryngeal initiation of swallowing. Exp. Neurol. 20, 359\u2013365 (1968).","journal-title":"Exp. Neurol."},{"key":"7400_CR9","doi-asserted-by":"publisher","first-page":"353","DOI":"10.5056\/jnm.2010.16.4.353","volume":"16","author":"H Miwa","year":"2010","unstructured":"Miwa, H. et al. Esophageal sensation and esophageal hypersensitivity: overview from bench to bedside. J. Neurogastroenterol. Motil. 16, 353\u2013362 (2010).","journal-title":"J. Neurogastroenterol. Motil."},{"key":"7400_CR10","doi-asserted-by":"publisher","first-page":"e50703","DOI":"10.1371\/journal.pone.0050703","volume":"7","author":"RM Mostafeezur","year":"2012","unstructured":"Mostafeezur, R. M. et al. Cannabinoids facilitate the swallowing reflex elicited by the superior laryngeal nerve stimulation in rats. PLoS ONE 7, e50703 (2012).","journal-title":"PLoS ONE"},{"key":"7400_CR11","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1113\/jphysiol.2003.039966","volume":"550","author":"S Jafari","year":"2003","unstructured":"Jafari, S., Prince, R. A., Kim, D. Y. & Paydarfar, D. Sensory regulation of swallowing and airway protection: a role for the internal superior laryngeal nerve in humans. J. Physiol. 550, 287\u2013304 (2003).","journal-title":"J. Physiol."},{"key":"7400_CR12","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1177\/000348940211100402","volume":"111","author":"L Sulica","year":"2002","unstructured":"Sulica, L., Hembree, A. & Blitzer, A. Swallowing and sensation: evaluation of deglutition in the anesthetized larynx. Ann. Otol. Rhinol. Laryngol. 111, 291\u2013294 (2002).","journal-title":"Ann. Otol. Rhinol. Laryngol."},{"key":"7400_CR13","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1152\/physrev.00005.2019","volume":"100","author":"K Talavera","year":"2020","unstructured":"Talavera, K. et al. Mammalian transient receptor potential TRPA1 channels: from structure to disease. Physiol. Rev. 100, 725\u2013803 (2020).","journal-title":"Physiol. Rev."},{"key":"7400_CR14","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1021\/cn500094a","volume":"5","author":"LS Premkumar","year":"2014","unstructured":"Premkumar, L. S. Transient receptor potential channels as targets for phytochemicals. ACS Chem. Neurosci. 5, 1117\u20131130 (2014).","journal-title":"ACS Chem. Neurosci."},{"key":"7400_CR15","doi-asserted-by":"publisher","first-page":"849","DOI":"10.1016\/S0896-6273(04)00150-3","volume":"41","author":"M Bandell","year":"2004","unstructured":"Bandell, M. et al. Noxious cold ion channel TRPA1 is activated by pungent compounds and bradykinin. Neuron 41, 849\u2013857 (2004).","journal-title":"Neuron"},{"key":"7400_CR16","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1038\/nature02282","volume":"427","author":"SE Jordt","year":"2004","unstructured":"Jordt, S. E. et al. Mustard oils and cannabinoids excite sensory nerve fibres through the TRP channel ANKTM1. Nature 427, 260\u2013265 (2004).","journal-title":"Nature"},{"key":"7400_CR17","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.brainres.2010.01.016","volume":"1319","author":"A Hondoh","year":"2010","unstructured":"Hondoh, A. et al. Distinct expression of cold receptors (TRPM8 and TRPA1) in the rat nodose-petrosal ganglion complex. Brain Res. 1319, 60\u201369 (2010).","journal-title":"Brain Res."},{"key":"7400_CR18","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1111\/nmo.12701","volume":"28","author":"D Alvarez-Berdugo","year":"2016","unstructured":"Alvarez-Berdugo, D. et al. Localization and expression of TRPV1 and TRPA1 in the human oropharynx and larynx. Neurogastroenterol. Motil. 28, 91\u2013100 (2016).","journal-title":"Neurogastroenterol. Motil."},{"key":"7400_CR19","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1016\/S0092-8674(03)00158-2","volume":"112","author":"GM Story","year":"2003","unstructured":"Story, G. M. et al. ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell 112, 819\u2013829 (2003).","journal-title":"Cell"},{"key":"7400_CR20","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1038\/nature03066","volume":"432","author":"DP Corey","year":"2004","unstructured":"Corey, D. P. et al. TRPA1 is a candidate for the mechanosensitive transduction channel of vertebrate hair cells. Nature 432, 723\u2013730 (2004).","journal-title":"Nature"},{"key":"7400_CR21","doi-asserted-by":"crossref","unstructured":"Kerstein, P. C., del Camino, D., Moran, M. M. & Stucky, C. L. Pharmacological blockade of TRPA1 inhibits mechanical firing in nociceptors. Mol. Pain 5, (2009).","DOI":"10.1186\/1744-8069-5-19"},{"key":"7400_CR22","doi-asserted-by":"publisher","first-page":"4808","DOI":"10.1523\/JNEUROSCI.5380-08.2009","volume":"29","author":"KY Kwan","year":"2009","unstructured":"Kwan, K. Y., Glazer, J. M., Corey, D. P., Rice, F. L. & Stucky, C. L. TRPA1 modulates mechanotransduction in cutaneous sensory neurons. J. Neurosci. 29, 4808\u20134819 (2009).","journal-title":"J. Neurosci."},{"key":"7400_CR23","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.1016\/j.cell.2006.02.023","volume":"124","author":"DM Bautista","year":"2006","unstructured":"Bautista, D. M. et al. TRPA1 mediates the inflammatory actions of environmental irritants and proalgesic agents. Cell 124, 1269\u20131282 (2006).","journal-title":"Cell"},{"key":"7400_CR24","doi-asserted-by":"publisher","first-page":"e13821","DOI":"10.1111\/nmo.13821","volume":"32","author":"N Tomsen","year":"2020","unstructured":"Tomsen, N. et al. A randomized clinical trial on the acute therapeutic effect of TRPA1 and TRPM8 agonists in patients with oropharyngeal dysphagia. Neurogastroenterol. Motil. 32, e13821 (2020).","journal-title":"Neurogastroenterol. Motil."},{"key":"7400_CR25","doi-asserted-by":"crossref","unstructured":"de Lama Lazzara, G., Lazarus, C. & Logemann, J. A. Impact of thermal stimulation on the triggering of the swallowing reflex. Dysphagia 1, 73\u201377 (1986).","DOI":"10.1007\/BF02407117"},{"key":"7400_CR26","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1044\/jshr.3406.1257","volume":"34","author":"JC Rosenbek","year":"1991","unstructured":"Rosenbek, J. C., Robbins, J., Fishback, B. & Levine, R. L. Effects of thermal application on dysphagia after stroke. J. Speech Hear. Res. 34, 1257\u20131268 (1991).","journal-title":"J. Speech Hear. Res."},{"key":"7400_CR27","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1007\/BF01540183","volume":"35","author":"CM Knauer","year":"1990","unstructured":"Knauer, C. M., Castell, J. A., Dalton, C. B., Nowak, L. & Castell, D. O. Pharyngeal\/upper esophageal sphincter pressure dynamics in humans - Effects of pharmacologic agents and thermal stimulation. Dig. Dis. Sci. 35, 774\u2013780 (1990).","journal-title":"Dig. Dis. Sci."},{"key":"7400_CR28","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1007\/BF00366386","volume":"11","author":"MN Kaatzke-McDonald","year":"1996","unstructured":"Kaatzke-McDonald, M. N., Post, E. & Davis, P. J. The effects of cold, touch, and chemical stimulation of the anterior faucial pillar on human swallowing. Dysphagia 11, 198\u2013206 (1996).","journal-title":"Dysphagia"},{"key":"7400_CR29","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1007\/BF00385791","volume":"11","author":"GN Ali","year":"1996","unstructured":"Ali, G. N., Laundl, T. M., Wallace, K. L., DeCarle, D. J. & Cook, I. J. S. Influence of cold stimulation on the normal pharyngeal swallow response. Dysphagia 11, 2\u20138 (1996).","journal-title":"Dysphagia"},{"key":"7400_CR30","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1007\/s00455-005-9003-6","volume":"21","author":"Y Miyaoka","year":"2006","unstructured":"Miyaoka, Y. et al. Influences of thermal and gustatory characteristics on sensory and motor aspects of swallowing. Dysphagia 21, 38\u201348 (2006).","journal-title":"Dysphagia"},{"key":"7400_CR31","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1682\/JRRD.2006.08.0089","volume":"44","author":"B Sel\u00e7uk","year":"2007","unstructured":"Sel\u00e7uk, B., Uysal, H., Aydogdu, I., Akyuz, M. & Ertekin, C. Effect of temperature on electrophysiological parameters of swallowing. J. Rehabil. Res. Dev. 44, 373\u2013380 (2007).","journal-title":"J. Rehabil. Res. Dev."},{"key":"7400_CR32","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.1044\/2018_JSLHR-S-17-0417","volume":"61","author":"IA Humbert","year":"2018","unstructured":"Humbert, I. A. et al. Swallowing kinematic differences across frozen, mixed, and ultrathin liquid boluses in healthy adults: age, sex, and normal variability. J. Speech Lang. Hear. Res. 61, 1544\u20131559 (2018).","journal-title":"J. Speech Lang. Hear. Res."},{"key":"7400_CR33","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1177\/0022034517695518","volume":"96","author":"MJ L\u00f3pez-Gonz\u00e1lez","year":"2017","unstructured":"L\u00f3pez-Gonz\u00e1lez, M. J. et al. TRPA1 Channels Mediate Human Gingival Fibroblast Response to Phenytoin. J. Dent. Res. 96, 832\u2013839 (2017).","journal-title":"J. Dent. Res."},{"key":"7400_CR34","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1177\/0022034514544507","volume":"93","author":"O Egbuniwe","year":"2014","unstructured":"Egbuniwe, O. et al. TRPA1 and TRPV4 activation in human odontoblasts stimulates ATP release. J. Dent. Res. 93, 911\u2013917 (2014).","journal-title":"J. Dent. Res."},{"key":"7400_CR35","doi-asserted-by":"publisher","first-page":"350","DOI":"10.3109\/10799893.2011.602413","volume":"31","author":"I Mukhopadhyay","year":"2011","unstructured":"Mukhopadhyay, I. et al. Expression of functional TRPA1 receptor on human lung fibroblast and epithelial cells. J. Recept. Signal Transduct. 31, 350\u2013358 (2011).","journal-title":"J. Recept. Signal Transduct."},{"key":"7400_CR36","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1016\/j.joen.2010.12.017","volume":"37","author":"IA El Karim","year":"2011","unstructured":"El Karim, I. A. et al. Human dental pulp fibroblasts express the \u2018cold-sensing\u2019 transient receptor potential channels TRPA1 and TRPM8. J. Endod. 37, 473\u2013478 (2011).","journal-title":"J. Endod."},{"key":"7400_CR37","doi-asserted-by":"publisher","first-page":"526","DOI":"10.3390\/ijms20030526","volume":"20","author":"MZ Hossain","year":"2019","unstructured":"Hossain, M. Z. et al. The role of transient receptor potential (TRP) channels in the transduction of dental pain. Int. J. Mol. Sci. 20, 526 (2019).","journal-title":"Int. J. Mol. Sci."},{"key":"7400_CR38","doi-asserted-by":"publisher","first-page":"e42454","DOI":"10.1371\/journal.pone.0042454","volume":"7","author":"R Nassini","year":"2012","unstructured":"Nassini, R. et al. Transient receptor potential ankyrin 1 channel localized to non-neuronal airway cells promotes non-neurogenic inflammation. PLoS ONE 7, e42454 (2012).","journal-title":"PLoS ONE"},{"key":"7400_CR39","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1111\/j.1471-4159.2011.07628.x","volume":"120","author":"Y Nakamura","year":"2012","unstructured":"Nakamura, Y. et al. Activation of transient receptor potential ankyrin 1 evokes nociception through substance P release from primary sensory neurons. J. Neurochem. 120, 1036\u20131047 (2012).","journal-title":"J. Neurochem."},{"key":"7400_CR40","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/0165-1838(84)90017-1","volume":"10","author":"A Jean","year":"1984","unstructured":"Jean, A. Control of the central swallowing program by inputs from the peripheral receptors: a review. J. Auton. Nerv. Syst. 10, 225\u2013233 (1984).","journal-title":"J. Auton. Nerv. Syst."},{"key":"7400_CR41","doi-asserted-by":"crossref","unstructured":"Bradley, R. M. Sensory receptors of the larynx. in American Journal of Medicine vol. 108 47\u201350 (Elsevier, 2000).","DOI":"10.1016\/S0002-9343(99)00339-3"},{"key":"7400_CR42","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1093\/chemse\/bjs004","volume":"37","author":"T Ohkuri","year":"2012","unstructured":"Ohkuri, T., Horio, N., Stratford, J. M., Finger, T. E. & Ninomiya, Y. Residual chemoresponsiveness to acids in the superior laryngeal nerve in \u2018taste-blind\u2019 (p2x2\/p2x3 double-ko) mice. Chem. Senses 37, 523\u2013532 (2012).","journal-title":"Chem. Senses"},{"key":"7400_CR43","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/0006-8993(83)90550-4","volume":"276","author":"RM Bradley","year":"1983","unstructured":"Bradley, R. M., Stedman, H. M. & Mistretta, C. M. Superior laryngeal nerve response patterns to chemical stimulation of sheep epiglottis. Brain Res. 276, 81\u201393 (1983).","journal-title":"Brain Res."},{"key":"7400_CR44","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.neulet.2007.02.032","volume":"417","author":"J Kitagawa","year":"2007","unstructured":"Kitagawa, J., Takahashi, Y., Matsumoto, S. & Shingai, T. Response properties of the pharyngeal branch of the glossopharyngeal nerve for umami taste in mice and rats. Neurosci. Lett. 417, 42\u201345 (2007).","journal-title":"Neurosci. Lett."},{"key":"7400_CR45","doi-asserted-by":"publisher","first-page":"4113","DOI":"10.3390\/ijms19124113","volume":"19","author":"MZ Hossain","year":"2018","unstructured":"Hossain, M. Z., Ando, H., Unno, S., Masuda, Y. & Kitagawa, J. Activation of TRPV1 and TRPM8 channels in the larynx and associated laryngopharyngeal regions facilitates the swallowing reflex. Int. J. Mol. Sci. 19, 4113 (2018).","journal-title":"Int. J. Mol. Sci."},{"key":"7400_CR46","doi-asserted-by":"publisher","first-page":"e13728","DOI":"10.1111\/nmo.13728","volume":"32","author":"MZ Hossain","year":"2020","unstructured":"Hossain, M. Z., Ando, H., Unno, S., Nakamoto, T. & Kitagawa, J. Functional involvement of acid-sensing ion channel 3 in the swallowing reflex in rats. Neurogastroenterol. Motil. 32, e13728 (2020).","journal-title":"Neurogastroenterol. Motil."},{"key":"7400_CR47","doi-asserted-by":"publisher","first-page":"1280","DOI":"10.1136\/gutjnl-2011-300753","volume":"62","author":"L Rofes","year":"2013","unstructured":"Rofes, L., Arreola, V., Martin, A. & Clav\u00e9, P. Natural capsaicinoids improve swallow response in older patients with oropharyngeal dysphagia. Gut 62, 1280\u20131287 (2013).","journal-title":"Gut"},{"key":"7400_CR48","doi-asserted-by":"publisher","first-page":"e13185","DOI":"10.1111\/nmo.13185","volume":"30","author":"D Alvarez-Berdugo","year":"2018","unstructured":"Alvarez-Berdugo, D. et al. A comparative study on the therapeutic effect of TRPV1, TRPA1, and TRPM8 agonists on swallowing dysfunction associated with aging and neurological diseases. Neurogastroenterol. Motil. 30, e13185 (2018).","journal-title":"Neurogastroenterol. Motil."},{"key":"7400_CR49","doi-asserted-by":"publisher","first-page":"175628481984204","DOI":"10.1177\/1756284819842043","volume":"12","author":"N Tomsen","year":"2019","unstructured":"Tomsen, N. et al. Acute and subacute effects of oropharyngeal sensory stimulation with TRPV1 agonists in older patients with oropharyngeal dysphagia: a biomechanical and neurophysiological randomized pilot study. Therap. Adv. Gastroenterol. 12, 1756284819842043 (2019).","journal-title":"Therap. Adv. Gastroenterol."},{"key":"7400_CR50","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1159\/000463382","volume":"95","author":"R Nakato","year":"2017","unstructured":"Nakato, R. et al. Effects of capsaicin on older patients with oropharyngeal dysphagia: a double-blind, placebo-controlled, crossover study. Digestion 95, 210\u2013220 (2017).","journal-title":"Digestion"},{"key":"7400_CR51","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1007\/s00535-013-0920-0","volume":"49","author":"L Rofes","year":"2014","unstructured":"Rofes, L., Arreola, V., Martin, A. & Clav\u00e9, P. Effect of oral piperine on the swallow response of patients with oropharyngeal dysphagia. J. Gastroenterol. 49, 1517\u20131523 (2014).","journal-title":"J. Gastroenterol."},{"key":"7400_CR52","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1016\/0140-6736(93)93023-T","volume":"341","author":"T Ebihara","year":"1993","unstructured":"Ebihara, T., Sekizawa, K., Nakazawa, H. & Sasaki, H. Capsaicin and swallowing reflex. Lancet 341, 432 (1993).","journal-title":"Lancet"},{"key":"7400_CR53","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1111\/j.1532-5415.2005.53261.x","volume":"53","author":"T Ebihara","year":"2005","unstructured":"Ebihara, T. et al. Capsaicin troche for swallowing dysfunction in older people. J. Am. Geriatr. Soc. 53, 824\u2013828 (2005).","journal-title":"J. Am. Geriatr. Soc."},{"key":"7400_CR54","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1111\/j.1532-5415.2006.00840.x","volume":"54","author":"T Ebihara","year":"2006","unstructured":"Ebihara, T. et al. A randomized trial of olfactory stimulation using black pepper oil in older people with swallowing dysfunction. J. Am. Geriatr. Soc. 54, 1401\u20131406 (2006).","journal-title":"J. Am. Geriatr. Soc."},{"key":"7400_CR55","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s42466-021-00122-3","volume":"3","author":"R Dziewas","year":"2021","unstructured":"Dziewas, R. et al. Diagnosis and treatment of neurogenic dysphagia: S1 guideline of the German Society of Neurology. Neurol. Res. Pract. 3, 23 (2021).","journal-title":"Neurol. Res. Pract."},{"key":"7400_CR56","doi-asserted-by":"crossref","unstructured":"Dziewas, R. et al. European stroke organisation and european society for swallowing disorders guideline for the diagnosis and treatment of post-stroke dysphagia. Eur. Stroke J. 6, LXXXIX\u2013CXV (2021).","DOI":"10.1177\/23969873211039721"},{"key":"7400_CR57","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1007\/BF00714591","volume":"9","author":"M Selinger","year":"1994","unstructured":"Selinger, M., Prescott, T. E. & Hoffman, I. Temperature acceleration in cold oral stimulation. Dysphagia 9, 83\u201387 (1994).","journal-title":"Dysphagia"},{"key":"7400_CR58","doi-asserted-by":"publisher","first-page":"2949","DOI":"10.1113\/JP277895","volume":"597","author":"T Tsujimura","year":"2019","unstructured":"Tsujimura, T. et al. Involvement of the epithelial sodium channel in initiation of mechanically evoked swallows in anaesthetized rats. J. Physiol. 597, 2949\u20132963 (2019).","journal-title":"J. Physiol."},{"key":"7400_CR59","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1007\/s10571-014-0062-9","volume":"34","author":"D Sato","year":"2014","unstructured":"Sato, D. et al. Distribution of TRPVs, P2X3, and parvalbumin in the human nodose ganglion. Cell. Mol. Neurobiol. 34, 851\u2013858 (2014).","journal-title":"Cell. Mol. Neurobiol."},{"key":"7400_CR60","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/S0304-3959(02)00067-2","volume":"99","author":"T Fukuoka","year":"2002","unstructured":"Fukuoka, T. et al. VR1, but not P2X3, increases in the spared L4 DRG in rats with L5 spinal nerve ligation. Pain 99, 111\u2013120 (2002).","journal-title":"Pain"},{"key":"7400_CR61","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1177\/0022034514565645","volume":"94","author":"K Urata","year":"2015","unstructured":"Urata, K. et al. Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain. J. Dent. Res. 94, 446\u2013454 (2015).","journal-title":"J. Dent. Res."},{"key":"7400_CR62","doi-asserted-by":"publisher","first-page":"2312","DOI":"10.1093\/brain\/awy177","volume":"141","author":"IM Marone","year":"2018","unstructured":"Marone, I. M. et al. TRPA1\/NOX in the soma of trigeminal ganglion neurons mediates migraine-related pain of glyceryl trinitrate in mice. Brain 141, 2312\u20132328 (2018).","journal-title":"Brain"},{"key":"7400_CR63","doi-asserted-by":"crossref","unstructured":"Toledo Mauri\u00f1o, J. J. et al. TRPV subfamily (TRPV2, TRPV3, TRPV4, TRPV5, and TRPV6) gene and protein expression in patients with ulcerative colitis. J. Immunol. Res. 2020, 1\u201311 (2020).","DOI":"10.1155\/2020\/2906845"},{"key":"7400_CR64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-017-01739-2","volume":"8","author":"F De Logu","year":"2017","unstructured":"De Logu, F. et al. Schwann cell TRPA1 mediates neuroinflammation that sustains macrophage-dependent neuropathic pain in mice. Nat. Commun. 8, 1\u201316 (2017).","journal-title":"Nat. Commun."},{"key":"7400_CR65","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1016\/j.eururo.2008.04.042","volume":"55","author":"C Gratzke","year":"2009","unstructured":"Gratzke, C. et al. Transient receptor potential A1 (TRPA1) activity in the human urethra-evidence for a functional role for TRPA1 in the outflow region. Eur. Urol. 55, 696\u2013704 (2009).","journal-title":"Eur. Urol."},{"key":"7400_CR66","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1186\/1746-160X-5-9","volume":"5","author":"M Vaiman","year":"2009","unstructured":"Vaiman, M. & Eviatar, E. Surface electromyography as a screening method for evaluation of dysphagia and odynophagia. Head Face Med. 5, 9 (2009).","journal-title":"Head Face Med."},{"key":"7400_CR67","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1152\/ajplegacy.1970.218.4.956","volume":"218","author":"WJ Sinclair","year":"1970","unstructured":"Sinclair, W. J. Initiation of reflex swallowing from the naso- and oropharynx. Am. J. Physiol. 218, 956\u2013960 (1970).","journal-title":"Am. J. Physiol."},{"key":"7400_CR68","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1124\/mol.107.040832","volume":"73","author":"TE Taylor-Clark","year":"2008","unstructured":"Taylor-Clark, T. E. et al. Prostaglandin-induced activation of nociceptive neurons via direct interaction with transient receptor potential A1 (TRPA1). Mol. Pharmacol. 73, 274\u2013281 (2008).","journal-title":"Mol. Pharmacol."},{"key":"7400_CR69","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1186\/1744-8069-4-48","volume":"4","author":"SR Eid","year":"2008","unstructured":"Eid, S. R. et al. HC-030031, a TRPA1 selective antagonist, attenuates inflammatory- and neuropathy-induced mechanical hypersensitivity. Mol. Pain 4, 48 (2008).","journal-title":"Mol. Pain"},{"key":"7400_CR70","doi-asserted-by":"publisher","first-page":"3929","DOI":"10.3390\/ijms21113929","volume":"21","author":"CL Chung","year":"2020","unstructured":"Chung, C. L., Lin, Y. S., Chan, N. J., Chen, Y. Y. & Hsu, C. C. Hypersensitivity of airway reflexes induced by hydrogen sulfide: role of TRPA1 receptors. Int. J. Mol. Sci. 21, 3929 (2020).","journal-title":"Int. J. Mol. Sci."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-07400-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-07400-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-07400-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T08:45:40Z","timestamp":1700297140000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41598-022-07400-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,2]]},"references-count":70,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["7400"],"URL":"https:\/\/doi.org\/10.1038\/s41598-022-07400-3","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,2]]},"assertion":[{"value":"30 September 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 February 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 March 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3431"}}