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In response, we assessed the performance and user preference of gargle specimens for qRT-PCR-based detection of SARS-CoV-2 in Indonesia. Inpatients who had recently been diagnosed with COVID-19 and outpatients who were about to perform qRT-PCR testing were asked to provide nasopharyngeal and oropharyngeal (NPOP) swabs and self-collected gargle specimens. We demonstrated that self-collected gargle specimens can be an alternative specimen to detect SARS-CoV-2 and the viral RNA remained stable for 31\u00a0days at room temperature storage. The developed method was validated for use on multiple RNA extraction kits and commercially available COVID-19 RT-PCR kits. Our developed method achieved a sensitivity of 91.38% when compared to paired NPOP swab specimens (Ct\u2009&lt;\u200935), with 97.10% of patients preferring the self-collected gargle method.<\/jats:p>","DOI":"10.1038\/s41598-022-07690-7","type":"journal-article","created":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T06:06:27Z","timestamp":1646719587000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Self-collected gargle specimen as a patient-friendly sample collection method for COVID-19 diagnosis in a population context"],"prefix":"10.1038","volume":"12","author":[{"given":"Revata","family":"Utama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebriarina","family":"Hapsari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iva","family":"Puspitasari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desvita","family":"Sari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meita","family":"Hendrianingtyas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neni","family":"Nurainy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,8]]},"reference":[{"issue":"3","key":"7690_CR1","doi-asserted-by":"publisher","first-page":"105924","DOI":"10.1016\/j.ijantimicag.2020.105924","volume":"55","author":"C Lai","year":"2020","unstructured":"Lai, C., Shih, T., Ko, W., Tang, H. & Hsueh, P. 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R.U. has financial holdings in Nusantics, PT Riset Nusantara Genetika. N.N. is an employee at PT. Biofarma. The remaining authors declare that they have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"3706"}}