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O-GlcNAcylation level is regulated by OGT (O-GlcNAc transferase), which adds GlcNAc on proteins, and OGA (O-GlcNAcase), which removes it. Abnormal level of protein<jats:italic>O<\/jats:italic>-GlcNAcylation has been observed in numerous cancer cell types, including cervical cancer cells. In the present study, we have evaluated the effect of increasing protein<jats:italic>O<\/jats:italic>-GlcNAcylation on cervical cancer-derived CaSki cells. We observed that pharmacological enhancement of protein<jats:italic>O<\/jats:italic>-GlcNAcylation by Thiamet G (an inhibitor of OGA) and glucosamine (which provides UDP-GlcNAc substrate to OGT) increases CaSki cells proliferation, migration and survival. Moreover, we showed that increased<jats:italic>O<\/jats:italic>-GlcNAcylation promotes IGF-1 receptor (IGF1R) autophosphorylation, possibly through inhibition of protein tyrosine-phosphatase 1B activity. This was associated with increased IGF-1-induced phosphatidyl-Inositol 3-phosphate production at the plasma membrane and increased Akt activation in CaSki cells. Finally, we showed that protein<jats:italic>O<\/jats:italic>-GlcNAcylation and Akt phosphorylation levels were higher in human cervical cancer samples compared to healthy cervix tissues, and a highly positive correlation was observed between<jats:italic>O<\/jats:italic>-GlcNAcylation level and Akt phosphorylation in theses tissues. Together, our results indicate that increased<jats:italic>O<\/jats:italic>-GlcNAcylation, by activating IGF1R\/ Phosphatidyl inositol 3-Kinase (PI-3K)\/Akt signaling, may participate in cervical cancer cell growth and proliferation.<\/jats:p>","DOI":"10.1038\/s41598-022-08445-0","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T11:03:55Z","timestamp":1647428635000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Increased O-GlcNAcylation promotes IGF-1 receptor\/PhosphatidyI Inositol-3 kinase\/Akt pathway in cervical cancer cells"],"prefix":"10.1038","volume":"12","author":[{"given":"Victoria","family":"Jim\u00e9nez-Castillo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Illescas-Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgar","family":"Zenteno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz X\u00f3chitl","family":"\u00c1vila-Curiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Casta\u00f1eda-Patl\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Robles-Flores","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Montante-Montes","family":"De Oca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"P\u00e9rez-Campos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anayetzin","family":"Torres-Rivera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelouhab","family":"Bouaboud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Pagesy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Josu\u00e9","family":"Sol\u00f3rzano-Mata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarik","family":"Issad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,16]]},"reference":[{"key":"8445_CR1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1016\/j.tem.2008.09.001","volume":"19","author":"T Issad","year":"2008","unstructured":"Issad, T. & Kuo, M. 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