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The purpose of this study was to establish a method for identifying and utilizing characteristic bacteria from the intestines of two algae-eating fish species:\n                    <jats:italic>Andamia tetradactylus<\/jats:italic>\n                    , which exclusively eats algae on the rock surface, and stellar rockskipper\n                    <jats:italic>Entomacrodus stellifer<\/jats:italic>\n                    , which feeds on both algae and invertebrates. We tested the species composition of the intestinal bacterial flora and found that Proteobacteria were commonly found both in species as in the common gut communities of marine fish, whereas Spirochaetes and Tenericutes occupied the flora of\n                    <jats:italic>A. tetradactylus<\/jats:italic>\n                    . We then performed anaerobic and aerobic cultures and isolated 34 and 44 strains including 48 strains belonged to\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species from\n                    <jats:italic>A. tetradactylus<\/jats:italic>\n                    and\n                    <jats:italic>E. stellifer<\/jats:italic>\n                    . We observed that some\n                    <jats:italic>Vibrio<\/jats:italic>\n                    strains formed a clear boundary to avoid contacting other strains of bacteria. Whole-genome sequencing of such two\n                    <jats:italic>Vibrio alginolyticus<\/jats:italic>\n                    strains revealed two cyclic chromosomes commonly found in the genome of\n                    <jats:italic>Vibrio<\/jats:italic>\n                    species, and some unique genes encoding alginate lyase, chitinases, and type I-F CRISPR-associated endoribonuclease for the first time in\n                    <jats:italic>Vibrio alginolyticus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-08511-7","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T14:06:01Z","timestamp":1647525961000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Gut microbiota analysis of Blenniidae fishes including an algae-eating fish and clear boundary formation among isolated Vibrio strains"],"prefix":"10.1038","volume":"12","author":[{"given":"Masa-aki","family":"Yoshida","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuma","family":"Tanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Akiyoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Kawamukai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"8511_CR1","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1093\/infdis\/150.2.213","volume":"150","author":"K Ramotar","year":"1984","unstructured":"Ramotar, K., Conly, J. 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