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As a result, a thorough understanding of ungulate malaria parasites, hosts, and mosquito vectors has been lacking, necessitating additional research efforts. This study aimed to identify the vector(s) of <jats:italic>Plasmodium bubalis<\/jats:italic>. A total of 187 female mosquitoes (133 <jats:italic>Anopheles<\/jats:italic> spp., 24 <jats:italic>Culex<\/jats:italic> spp., 24 <jats:italic>Aedes<\/jats:italic> spp., and 6 <jats:italic>Mansonia<\/jats:italic> spp. collected from a buffalo farm in Thailand where concurrently collected water buffalo samples were examined and we found only <jats:italic>Anopheles<\/jats:italic> spp. samples were <jats:italic>P. bubalis<\/jats:italic> positive. Molecular identification of anopheline mosquito species was conducted by sequencing of the PCR products targeting <jats:italic>cytochrome c oxidase subunit 1<\/jats:italic> (<jats:italic>cox1<\/jats:italic>), <jats:italic>cytochrome c oxidase subunit 2<\/jats:italic> (<jats:italic>cox2<\/jats:italic>), and internal transcribed spacer 2 (<jats:italic>ITS2<\/jats:italic>) markers. We observed 5 distinct groups of anopheline mosquitoes: Barbirostris, Hyrcanus, Ludlowae, Funestus, and Jamesii groups. The Barbirostris group (<jats:italic>Anopheles wejchoochotei<\/jats:italic> or <jats:italic>Anopheles campestris<\/jats:italic>) and the Hyrcanus group (<jats:italic>Anopheles peditaeniatus<\/jats:italic>) were positive for <jats:italic>P. bubalis<\/jats:italic>. Thus, for the first time, our study implicated these anopheline mosquito species as probable vectors of <jats:italic>P. bubalis<\/jats:italic> in Thailand.<\/jats:p>","DOI":"10.1038\/s41598-022-09686-9","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T09:03:04Z","timestamp":1649235784000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Myzorhynchus series of Anopheles mosquitoes as potential vectors of Plasmodium bubalis in Thailand"],"prefix":"10.1038","volume":"12","author":[{"given":"Yudhi Ratna","family":"Nugraheni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Apinya","family":"Arnuphapprasert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trang Thuy","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duriyang","family":"Narapakdeesakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hoang Lan Anh","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juthathip","family":"Poofery","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahito","family":"Asada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morakot","family":"Kaewthamasorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"9686_CR1","doi-asserted-by":"publisher","first-page":"1501","DOI":"10.1017\/S0031182016001141","volume":"143","author":"TJ Templeton","year":"2016","unstructured":"Templeton, T. 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