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Our group showed that articular cartilage can be quantitatively assessed in nuclear medicine imaging by our radiotracer <jats:sup>99m<\/jats:sup>Tc-NTP 15-5 targeting cartilage proteoglycans. In this work, surgically induced DMM mice were treated with sprifermin or saline. We investigated cartilage remodelling in the mice knees by <jats:sup>99m<\/jats:sup>Tc-NTP 15-5 SPECT-CT imaging over 24\u00a0weeks after surgery, as wells as proteoglycan biochemical assays. OA alterations were scored by histology according to OARSI guidelines. A specific accumulation of <jats:sup>99m<\/jats:sup>Tc-NTP 15-5 in cartilage joints was evidenced in vivo by SPECT-CT imaging as early as 30\u00a0min post-iv injection. In DMM, <jats:sup>99m<\/jats:sup>Tc-NTP 15-5 accumulation in cartilage within the operated joints, relative to contralateral ones, was observed to initially increase then decrease as pathology progressed. Under sprifermin, <jats:sup>99m<\/jats:sup>Tc-NTP 15-5 uptake in pathological knees was significantly increased compared to controls, at 7-, 12- and 24-weeks, and consistent with proteoglycan increase measured 5\u00a0weeks post-surgery, as a sign of cartilage matrix remodelling. Our work highlights the potential of <jats:sup>99m<\/jats:sup>Tc-NTP 15-5 as an imaging-based companion to monitor cartilage remodelling in OA and DMOAD response.<\/jats:p>","DOI":"10.1038\/s41598-022-11080-4","type":"journal-article","created":{"date-parts":[[2022,5,17]],"date-time":"2022-05-17T10:09:46Z","timestamp":1652782186000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["99mTc-NTP 15-5 is a companion radiotracer for assessing joint functional response to sprifermin (rhFGF-18) in a murine osteoarthritis model"],"prefix":"10.1038","volume":"12","author":[{"given":"Arnaud","family":"Briat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire","family":"Jacques","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e9lodie","family":"Malige","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laure","family":"Sudre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geoffroy","family":"Nourissat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Auzeloux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Guehring","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florent","family":"Cachin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francis","family":"Berenbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth","family":"Miot-Noirault","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2022,5,17]]},"reference":[{"key":"11080_CR1","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.joca.2020.01.002","volume":"28","author":"M Kloppenburg","year":"2020","unstructured":"Kloppenburg, M. & Berenbaum, F. 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