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Carbapenem-resistant Gram-negative bacterial (CR-GNB) infection has become a fast-expanding global threat with limited antibiotic choice and significant mortality. This study aimed to highlight the carbapenem-resistance among clinical isolates of hospital admitted patients in Bihar, India. A cross-sectional study was conducted with 101 clinical isolates of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    , and\n                    <jats:italic>Pseudomonas aeruginosa.<\/jats:italic>\n                    All GNB isolates were tested for their antimicrobial susceptibility using Kirby-Bauer disc diffusion method. Double disc synergy test \/ modified Hodge test (DDST\/MHT) were used to detect carbapenemase production by these isolates. Subsequently, these isolates were evaluated for carbapenem-resistance genes using whole-genome sequencing method. The overall percentage of carbapenem-resistance among GNB was (17\/101) 16.8%. The genomic analysis of antimicrobial-resistance (AMR) demonstrates a significantly high prevalence of bla\n                    <jats:sub>CTX-M<\/jats:sub>\n                    followed by bla\n                    <jats:sub>SHV<\/jats:sub>\n                    , bla\n                    <jats:sub>TEM<\/jats:sub>\n                    , bla\n                    <jats:sub>OXA,<\/jats:sub>\n                    and bla\n                    <jats:sub>NDM<\/jats:sub>\n                    \u03b2-lactam or carbapenem resistance genes among clinical isolates of GNB. Co-occurrence of bla\n                    <jats:sub>NDM<\/jats:sub>\n                    with other beta-lactamase-encoding genes was found in 70.6% of carbapenemase-producing isolates. Our study highlights the mechanism of carbapenem-resistance to curb the overwhelming threat posed by the emergence of drug-resistance in India.\n                  <\/jats:p>","DOI":"10.1038\/s41598-022-12471-3","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T10:03:50Z","timestamp":1652954630000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Genome-wide identification of carbapenem-resistant Gram-negative bacterial (CR-GNB) isolates retrieved from hospitalized patients in Bihar, India"],"prefix":"10.1038","volume":"12","author":[{"given":"Namrata","family":"Kumari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukesh","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Katiyar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhay","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallavi","family":"Priya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bablu","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nihar Ranjan","family":"Biswas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Punit","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"12471_CR1","doi-asserted-by":"publisher","first-page":"8299","DOI":"10.1038\/s41598-020-64934-0","volume":"10","author":"A Katiyar","year":"2020","unstructured":"Katiyar, A. et al. 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